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A thin-reflector microfluidic resonator for continuous-flow concentration of microorganisms: a new approach to water quality analysis using acoustofluidics

A thin-reflector microfluidic resonator for continuous-flow concentration of microorganisms: a new approach to water quality analysis using acoustofluidics
A thin-reflector microfluidic resonator for continuous-flow concentration of microorganisms: a new approach to water quality analysis using acoustofluidics
An acoustofluidic device has been developed for concentrating vegetative bacteria in a continuous-flow format. We show that it is possible to overcome the disruptive effects of acoustic streaming which typically dominate for small target particles{,} and demonstrate flow rates compatible with the testing of drinking water. The device consists of a thin-reflector multi-layered resonator{,} in which bacteria in suspension are levitated towards a glass surface under the action of acoustic radiation forces. In order to achieve robust device performance over long-term operation{,} functional tests have been carried out to (i) maintain device integrity over time and stabilise its resonance frequency{,} (ii) optimise the operational acoustic parameters{,} and (iii) minimise bacterial adhesion on the inner surfaces. Using the developed device{,} a significant increase in bacterial concentration has been achieved{,} up to a maximum of ~60-fold. The concentration performance of thin-reflector resonators was found to be superior to comparable half-wave resonators.
1473-0197
3830-3842
Carugo, Dario
0a4be6cd-e309-4ed8-a620-20256ce01179
Octon, Tobias
e929d162-ebc7-4361-9bc1-879e263164a5
Messaoudi, Walid
867be685-0f23-4b39-bd8e-dd7df8402375
Fisher, Adam
0cbfd7a8-a475-45eb-bf51-3f5377645c51
Carboni, Michele
e3ef8207-6a01-4f2f-a498-37e8ac46a6eb
Harris, Nick
237cfdbd-86e4-4025-869c-c85136f14dfd
Hill, Martyn
0cda65c8-a70f-476f-b126-d2c4460a253e
Glynne-Jones, Peter
6ca3fcbc-14db-4af9-83e2-cf7c8b91ef0d
Carugo, Dario
0a4be6cd-e309-4ed8-a620-20256ce01179
Octon, Tobias
e929d162-ebc7-4361-9bc1-879e263164a5
Messaoudi, Walid
867be685-0f23-4b39-bd8e-dd7df8402375
Fisher, Adam
0cbfd7a8-a475-45eb-bf51-3f5377645c51
Carboni, Michele
e3ef8207-6a01-4f2f-a498-37e8ac46a6eb
Harris, Nick
237cfdbd-86e4-4025-869c-c85136f14dfd
Hill, Martyn
0cda65c8-a70f-476f-b126-d2c4460a253e
Glynne-Jones, Peter
6ca3fcbc-14db-4af9-83e2-cf7c8b91ef0d

Carugo, Dario, Octon, Tobias, Messaoudi, Walid, Fisher, Adam, Carboni, Michele, Harris, Nick, Hill, Martyn and Glynne-Jones, Peter (2014) A thin-reflector microfluidic resonator for continuous-flow concentration of microorganisms: a new approach to water quality analysis using acoustofluidics. Lab on a Chip, (19), 3830-3842. (doi:10.1039/C4LC00577E).

Record type: Article

Abstract

An acoustofluidic device has been developed for concentrating vegetative bacteria in a continuous-flow format. We show that it is possible to overcome the disruptive effects of acoustic streaming which typically dominate for small target particles{,} and demonstrate flow rates compatible with the testing of drinking water. The device consists of a thin-reflector multi-layered resonator{,} in which bacteria in suspension are levitated towards a glass surface under the action of acoustic radiation forces. In order to achieve robust device performance over long-term operation{,} functional tests have been carried out to (i) maintain device integrity over time and stabilise its resonance frequency{,} (ii) optimise the operational acoustic parameters{,} and (iii) minimise bacterial adhesion on the inner surfaces. Using the developed device{,} a significant increase in bacterial concentration has been achieved{,} up to a maximum of ~60-fold. The concentration performance of thin-reflector resonators was found to be superior to comparable half-wave resonators.

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Accepted/In Press date: 5 August 2014
Published date: 5 August 2014
Organisations: EEE

Identifiers

Local EPrints ID: 367737
URI: http://eprints.soton.ac.uk/id/eprint/367737
ISSN: 1473-0197
PURE UUID: ec095a60-d3d1-474c-b2c5-77845240c423
ORCID for Nick Harris: ORCID iD orcid.org/0000-0003-4122-2219
ORCID for Martyn Hill: ORCID iD orcid.org/0000-0001-6448-9448
ORCID for Peter Glynne-Jones: ORCID iD orcid.org/0000-0001-5684-3953

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Date deposited: 06 Aug 2014 10:08
Last modified: 03 Dec 2019 02:05

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