Biomolecular layer thickness evaluation using White Light Reflectance Spectroscopy
Biomolecular layer thickness evaluation using White Light Reflectance Spectroscopy
A White Light Reflectance Spectroscopy (WLRS) methodology is applied in the evaluation of the effectiveness of biomolecules immobilization onto solid surfaces as well as their subsequent reaction with counterpart biomolecules via measuring the respective layers thickness. In particular we investigated the adsorption of rabbit and mouse gamma-globulins as well as their reaction with complementary antibodies. The results obtained with the proposed methodology were compared with those received by atomic force microscopy analysis of the same samples. It was found that the developed method provides a simple, very fast and accurate approach for thin biomolecular layers thickness determination.
reflectance spectroscopy, atomic force microscopy, biomolecular layer, gamma-globulins
802-805
Kitsara, M.
38c96699-e86e-406f-a37a-dae9637892a5
Petrou, P.
13296e4e-ceb6-4850-b0e7-1c7104e9ed37
Kontziampasis, D.
0bffea5c-c766-4f82-b153-e5c00ac3df33
Misiakos, K.
4b3244d9-15da-47f2-8861-5886dbe39f82
Makarona, E.
6b92d175-cd5e-41c9-ba38-d6daef2b905c
Raptis, I.
9a7d2854-7e9e-42f4-a6f2-bc7835d9aa72
Beltsios, K.
3f4b34f6-0a28-4c64-8cba-0aec8422a95d
May 2010
Kitsara, M.
38c96699-e86e-406f-a37a-dae9637892a5
Petrou, P.
13296e4e-ceb6-4850-b0e7-1c7104e9ed37
Kontziampasis, D.
0bffea5c-c766-4f82-b153-e5c00ac3df33
Misiakos, K.
4b3244d9-15da-47f2-8861-5886dbe39f82
Makarona, E.
6b92d175-cd5e-41c9-ba38-d6daef2b905c
Raptis, I.
9a7d2854-7e9e-42f4-a6f2-bc7835d9aa72
Beltsios, K.
3f4b34f6-0a28-4c64-8cba-0aec8422a95d
Kitsara, M., Petrou, P., Kontziampasis, D., Misiakos, K., Makarona, E., Raptis, I. and Beltsios, K.
(2010)
Biomolecular layer thickness evaluation using White Light Reflectance Spectroscopy.
[in special issue: The 35th International Conference on Micro- and Nano-Engineering (MNE)]
Microelectronic Engineering, 87 (5-8), .
(doi:10.1016/j.mee.2009.11.082).
Abstract
A White Light Reflectance Spectroscopy (WLRS) methodology is applied in the evaluation of the effectiveness of biomolecules immobilization onto solid surfaces as well as their subsequent reaction with counterpart biomolecules via measuring the respective layers thickness. In particular we investigated the adsorption of rabbit and mouse gamma-globulins as well as their reaction with complementary antibodies. The results obtained with the proposed methodology were compared with those received by atomic force microscopy analysis of the same samples. It was found that the developed method provides a simple, very fast and accurate approach for thin biomolecular layers thickness determination.
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e-pub ahead of print date: 22 November 2009
Published date: May 2010
Keywords:
reflectance spectroscopy, atomic force microscopy, biomolecular layer, gamma-globulins
Organisations:
Nanoelectronics and Nanotechnology
Identifiers
Local EPrints ID: 368025
URI: http://eprints.soton.ac.uk/id/eprint/368025
ISSN: 0167-9317
PURE UUID: 3f0c705f-ff8c-49c1-a7c9-d74ca33db195
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Date deposited: 10 Sep 2014 13:40
Last modified: 14 Mar 2024 17:40
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Contributors
Author:
M. Kitsara
Author:
P. Petrou
Author:
D. Kontziampasis
Author:
K. Misiakos
Author:
E. Makarona
Author:
I. Raptis
Author:
K. Beltsios
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