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Biomolecular layer thickness evaluation using White Light Reflectance Spectroscopy

Biomolecular layer thickness evaluation using White Light Reflectance Spectroscopy
Biomolecular layer thickness evaluation using White Light Reflectance Spectroscopy
A White Light Reflectance Spectroscopy (WLRS) methodology is applied in the evaluation of the effectiveness of biomolecules immobilization onto solid surfaces as well as their subsequent reaction with counterpart biomolecules via measuring the respective layers thickness. In particular we investigated the adsorption of rabbit and mouse gamma-globulins as well as their reaction with complementary antibodies. The results obtained with the proposed methodology were compared with those received by atomic force microscopy analysis of the same samples. It was found that the developed method provides a simple, very fast and accurate approach for thin biomolecular layers thickness determination.
reflectance spectroscopy, atomic force microscopy, biomolecular layer, gamma-globulins
0167-9317
802-805
Kitsara, M.
38c96699-e86e-406f-a37a-dae9637892a5
Petrou, P.
13296e4e-ceb6-4850-b0e7-1c7104e9ed37
Kontziampasis, D.
0bffea5c-c766-4f82-b153-e5c00ac3df33
Misiakos, K.
4b3244d9-15da-47f2-8861-5886dbe39f82
Makarona, E.
6b92d175-cd5e-41c9-ba38-d6daef2b905c
Raptis, I.
9a7d2854-7e9e-42f4-a6f2-bc7835d9aa72
Beltsios, K.
3f4b34f6-0a28-4c64-8cba-0aec8422a95d
Kitsara, M.
38c96699-e86e-406f-a37a-dae9637892a5
Petrou, P.
13296e4e-ceb6-4850-b0e7-1c7104e9ed37
Kontziampasis, D.
0bffea5c-c766-4f82-b153-e5c00ac3df33
Misiakos, K.
4b3244d9-15da-47f2-8861-5886dbe39f82
Makarona, E.
6b92d175-cd5e-41c9-ba38-d6daef2b905c
Raptis, I.
9a7d2854-7e9e-42f4-a6f2-bc7835d9aa72
Beltsios, K.
3f4b34f6-0a28-4c64-8cba-0aec8422a95d

Kitsara, M., Petrou, P., Kontziampasis, D., Misiakos, K., Makarona, E., Raptis, I. and Beltsios, K. (2010) Biomolecular layer thickness evaluation using White Light Reflectance Spectroscopy. [in special issue: The 35th International Conference on Micro- and Nano-Engineering (MNE)] Microelectronic Engineering, 87 (5-8), 802-805. (doi:10.1016/j.mee.2009.11.082).

Record type: Article

Abstract

A White Light Reflectance Spectroscopy (WLRS) methodology is applied in the evaluation of the effectiveness of biomolecules immobilization onto solid surfaces as well as their subsequent reaction with counterpart biomolecules via measuring the respective layers thickness. In particular we investigated the adsorption of rabbit and mouse gamma-globulins as well as their reaction with complementary antibodies. The results obtained with the proposed methodology were compared with those received by atomic force microscopy analysis of the same samples. It was found that the developed method provides a simple, very fast and accurate approach for thin biomolecular layers thickness determination.

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More information

e-pub ahead of print date: 22 November 2009
Published date: May 2010
Keywords: reflectance spectroscopy, atomic force microscopy, biomolecular layer, gamma-globulins
Organisations: Nanoelectronics and Nanotechnology

Identifiers

Local EPrints ID: 368025
URI: http://eprints.soton.ac.uk/id/eprint/368025
ISSN: 0167-9317
PURE UUID: 3f0c705f-ff8c-49c1-a7c9-d74ca33db195

Catalogue record

Date deposited: 10 Sep 2014 13:40
Last modified: 15 Jul 2019 21:47

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