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Approach to engineer tomato by expression of AtHMA4 to enhance Zn in the aerial parts

Approach to engineer tomato by expression of AtHMA4 to enhance Zn in the aerial parts
Approach to engineer tomato by expression of AtHMA4 to enhance Zn in the aerial parts
The aim of this work was to assess the potential for using AtHMA4 to engineer enhanced efficiency of Zn translocation to shoots, and to increase the Zn concentration in aerial tissues of tomato. AtHMA4, a P1B-ATPase, encodes a Zn export protein known to be involved in the control of Zn root-to-shoot translocation. In this work, 35S::AtHMA4 was expressed in tomato (Lycopersicon esculentum var. Beta). Wild-type and transgenic plants were tested for Zn and Cd tolerance; Zn, Fe and Cd accumulation patterns, and for the expression of endogenous Zn/Fe-homeostasis genes. At 10 ?M Zn exposure, a higher Zn concentration was observed in leaves of AtHMA4-expressing lines compared to wild-type, which is promising in terms of Zn biofortification. AtHMA4 also transports Cd and at 0.25 ?M Cd the transgenic plants showed similar levels of this element in leaves to wild-type but lower levels in roots, therefore indicating a reduction of Cd uptake due to AtHMA4 expression. Expression of this transgene AtHMA4 also resulted in distinct changes in Fe accumulation in Zn-exposed plants, and Fe/Zn-accumulation in Cd-exposed plants, even though Fe is not a substrate for AtHMA4. Analysis of the transcript abundance of key Zn/Fe-homeostasis genes showed that the pattern was distinct for transgenic and wild-type plants. The reduction of Fe accumulation observed in AtHMA4-transformants was accompanied by up-regulation of Fe-deficiency marker genes (LeFER, LeFRO1, LeIRT1), whereas down-regulation was detected in plants with the status of Fe-sufficiency. Furthermore, results strongly suggest the importance of the up-regulation of LeCHLN in the roots of AtHMA4-expressing plants for efficient translocation of Zn to the shoots. Thus, the modifications of Zn/Fe/Cd translocation to aerial plant parts due to AtHMA4 expression are closely related to the alteration of the endogenous Zn–Fe–Cd cross-homeostasis network of tomato.
cadmium, AtHMA4, biofortification, tomato
0176-1617
1413-1422
Kendziorek, Maria
dd1ce2ec-4b2f-4dae-b4c5-437901fc7c33
Barabasz, Anna
0e750db4-b7da-4456-8465-426452b89a2b
Rudzka, Justyna
1079d2b3-09cd-43e0-b84c-b0a802a312d5
Tracz, Katarzyna
50e41a50-70ad-4a78-9d63-767a2f32131f
Mills, Rebecca F.
dec4afc8-be3b-4517-9038-c3c3e8b71b35
Williams, Lorraine E.
79ee1856-3732-492b-8ac5-239749c85d9e
Antosiewicz, Danuta Maria
cbbcbcfe-2ce6-4737-9933-304d48a1590b
Kendziorek, Maria
dd1ce2ec-4b2f-4dae-b4c5-437901fc7c33
Barabasz, Anna
0e750db4-b7da-4456-8465-426452b89a2b
Rudzka, Justyna
1079d2b3-09cd-43e0-b84c-b0a802a312d5
Tracz, Katarzyna
50e41a50-70ad-4a78-9d63-767a2f32131f
Mills, Rebecca F.
dec4afc8-be3b-4517-9038-c3c3e8b71b35
Williams, Lorraine E.
79ee1856-3732-492b-8ac5-239749c85d9e
Antosiewicz, Danuta Maria
cbbcbcfe-2ce6-4737-9933-304d48a1590b

Kendziorek, Maria, Barabasz, Anna, Rudzka, Justyna, Tracz, Katarzyna, Mills, Rebecca F., Williams, Lorraine E. and Antosiewicz, Danuta Maria (2014) Approach to engineer tomato by expression of AtHMA4 to enhance Zn in the aerial parts. Journal of Plant Physiology, 171 (15), 1413-1422. (doi:10.1016/j.jplph.2014.04.017). (PMID:25046762)

Record type: Article

Abstract

The aim of this work was to assess the potential for using AtHMA4 to engineer enhanced efficiency of Zn translocation to shoots, and to increase the Zn concentration in aerial tissues of tomato. AtHMA4, a P1B-ATPase, encodes a Zn export protein known to be involved in the control of Zn root-to-shoot translocation. In this work, 35S::AtHMA4 was expressed in tomato (Lycopersicon esculentum var. Beta). Wild-type and transgenic plants were tested for Zn and Cd tolerance; Zn, Fe and Cd accumulation patterns, and for the expression of endogenous Zn/Fe-homeostasis genes. At 10 ?M Zn exposure, a higher Zn concentration was observed in leaves of AtHMA4-expressing lines compared to wild-type, which is promising in terms of Zn biofortification. AtHMA4 also transports Cd and at 0.25 ?M Cd the transgenic plants showed similar levels of this element in leaves to wild-type but lower levels in roots, therefore indicating a reduction of Cd uptake due to AtHMA4 expression. Expression of this transgene AtHMA4 also resulted in distinct changes in Fe accumulation in Zn-exposed plants, and Fe/Zn-accumulation in Cd-exposed plants, even though Fe is not a substrate for AtHMA4. Analysis of the transcript abundance of key Zn/Fe-homeostasis genes showed that the pattern was distinct for transgenic and wild-type plants. The reduction of Fe accumulation observed in AtHMA4-transformants was accompanied by up-regulation of Fe-deficiency marker genes (LeFER, LeFRO1, LeIRT1), whereas down-regulation was detected in plants with the status of Fe-sufficiency. Furthermore, results strongly suggest the importance of the up-regulation of LeCHLN in the roots of AtHMA4-expressing plants for efficient translocation of Zn to the shoots. Thus, the modifications of Zn/Fe/Cd translocation to aerial plant parts due to AtHMA4 expression are closely related to the alteration of the endogenous Zn–Fe–Cd cross-homeostasis network of tomato.

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More information

e-pub ahead of print date: 12 June 2014
Published date: 15 September 2014
Keywords: cadmium, AtHMA4, biofortification, tomato
Organisations: Centre for Biological Sciences

Identifiers

Local EPrints ID: 368040
URI: http://eprints.soton.ac.uk/id/eprint/368040
ISSN: 0176-1617
PURE UUID: 4655cfe1-5726-4c62-900c-97d962cd3cb6

Catalogue record

Date deposited: 14 Aug 2014 09:35
Last modified: 05 Oct 2018 12:09

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