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Sensitivity of housekeeping genes in the suprachiasmatic nucleus of the mouse brain to diet and the daily light–dark cycle

Sensitivity of housekeeping genes in the suprachiasmatic nucleus of the mouse brain to diet and the daily light–dark cycle
Sensitivity of housekeeping genes in the suprachiasmatic nucleus of the mouse brain to diet and the daily light–dark cycle
The endogenous timing system within the suprachiasmatic nuclei (SCN) of the hypothalamus drives the cyclic expression of the clock molecules across the 24 h day–night cycle controlling downstream molecular pathways and physiological processes. The developing fetal clock system is sensitive to the environment and physiology of the pregnant mother and as such disruption of this system could lead to altered physiology in the offspring. Characterizing the gene profiles of the endogenous molecular clock system by quantitative reverse transcription polymerase chain reaction is dependent on normalization by appropriate housekeeping genes (HKGs). However, many HKGs commonly used as internal controls, although stably expressed under control conditions, can vary significantly in their expression under certain experimental conditions. Here we analyzed the expression of 10 classic HKG across the 24 h light–dark cycle in the SCN of mouse offspring exposed to normal chow or a high fat diet during early development and in postnatal life. We found that the HKGs glyceraldehyde-3-phosphate dehydrogenase, beta actin and adenosine triphosphate synthase subunit to be the most stably expressed genes in the SCN regardless of diet or time within the 24 h light–dark cycle, and are therefore suitable to be used as internal controls. However SCN samples collected during the light and dark periods did show differences in expression and as such the timing of collection should be considered when carrying out gene expression studies
0006-8993
72-77
Cleal, Jane
18cfd2c1-bd86-4a13-b38f-c321af56da66
Shepherd, James N.
c9084ea0-50a7-4518-b418-97c7cd76f65d
Shearer, Jasmine L.
c9599d59-4fc0-4fe7-ada2-2a8acd88dae5
BRUCE, KIMBERLEY D
1decbcba-5842-4d38-ae16-b540b7972dc6
Cagampang, Felino R.
7cf57d52-4a65-4554-8306-ed65226bc50e
Cleal, Jane
18cfd2c1-bd86-4a13-b38f-c321af56da66
Shepherd, James N.
c9084ea0-50a7-4518-b418-97c7cd76f65d
Shearer, Jasmine L.
c9599d59-4fc0-4fe7-ada2-2a8acd88dae5
BRUCE, KIMBERLEY D
1decbcba-5842-4d38-ae16-b540b7972dc6
Cagampang, Felino R.
7cf57d52-4a65-4554-8306-ed65226bc50e

Cleal, Jane, Shepherd, James N., Shearer, Jasmine L., BRUCE, KIMBERLEY D and Cagampang, Felino R. (2014) Sensitivity of housekeeping genes in the suprachiasmatic nucleus of the mouse brain to diet and the daily light–dark cycle. Brain Research, 1575, 72-77. (doi:10.1016/j.brainres.2014.05.031).

Record type: Article

Abstract

The endogenous timing system within the suprachiasmatic nuclei (SCN) of the hypothalamus drives the cyclic expression of the clock molecules across the 24 h day–night cycle controlling downstream molecular pathways and physiological processes. The developing fetal clock system is sensitive to the environment and physiology of the pregnant mother and as such disruption of this system could lead to altered physiology in the offspring. Characterizing the gene profiles of the endogenous molecular clock system by quantitative reverse transcription polymerase chain reaction is dependent on normalization by appropriate housekeeping genes (HKGs). However, many HKGs commonly used as internal controls, although stably expressed under control conditions, can vary significantly in their expression under certain experimental conditions. Here we analyzed the expression of 10 classic HKG across the 24 h light–dark cycle in the SCN of mouse offspring exposed to normal chow or a high fat diet during early development and in postnatal life. We found that the HKGs glyceraldehyde-3-phosphate dehydrogenase, beta actin and adenosine triphosphate synthase subunit to be the most stably expressed genes in the SCN regardless of diet or time within the 24 h light–dark cycle, and are therefore suitable to be used as internal controls. However SCN samples collected during the light and dark periods did show differences in expression and as such the timing of collection should be considered when carrying out gene expression studies

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More information

e-pub ahead of print date: 1 June 2014
Published date: 5 August 2014
Organisations: Faculty of Medicine, Human Development & Health

Identifiers

Local EPrints ID: 368131
URI: http://eprints.soton.ac.uk/id/eprint/368131
ISSN: 0006-8993
PURE UUID: 6fdaa968-bdc7-49ab-9050-ab6fb4a03347
ORCID for Jane Cleal: ORCID iD orcid.org/0000-0001-7978-4327
ORCID for Felino R. Cagampang: ORCID iD orcid.org/0000-0003-4404-9853

Catalogue record

Date deposited: 18 Aug 2014 11:57
Last modified: 18 Feb 2021 16:59

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