A sheath-less combined optical and impedance micro-cytometer
A sheath-less combined optical and impedance micro-cytometer
We describe a sheath-less micro-cytometer that measures four different parameters, namely fluorescence, large angle side scatter and dual frequency electrical impedance (electrical volume and opacity). The cytometer was benchmarked using both size and fluorescent bead standards and demonstrates excellent size accuracy (CVs ? 2.1%), sensitivity and dynamic range (3.5 orders of magnitude) at sample flow rates of 80 ?L per minute. The cytometer was evaluated by analysing human blood, and a four part differential leukocyte assay for accurate CD4+ T-cell enumeration was demonstrated. The integration of impedance, fluorescence and side scatter into a single miniature cytometer platform provides the core information content of a classical cytometer in a highly compact, simple, portable and low cost format.
3064-3073
Spencer, Daniel
4affe9f6-353a-4507-8066-0180b8dc9eaf
Elliott, Gregor
68369911-4f4e-436e-9fdb-3434636eab5a
Morgan, Hywel
de00d59f-a5a2-48c4-a99a-1d5dd7854174
2014
Spencer, Daniel
4affe9f6-353a-4507-8066-0180b8dc9eaf
Elliott, Gregor
68369911-4f4e-436e-9fdb-3434636eab5a
Morgan, Hywel
de00d59f-a5a2-48c4-a99a-1d5dd7854174
Spencer, Daniel, Elliott, Gregor and Morgan, Hywel
(2014)
A sheath-less combined optical and impedance micro-cytometer.
Lab on a Chip, 14 (16), .
(doi:10.1039/c4lc00224e).
Abstract
We describe a sheath-less micro-cytometer that measures four different parameters, namely fluorescence, large angle side scatter and dual frequency electrical impedance (electrical volume and opacity). The cytometer was benchmarked using both size and fluorescent bead standards and demonstrates excellent size accuracy (CVs ? 2.1%), sensitivity and dynamic range (3.5 orders of magnitude) at sample flow rates of 80 ?L per minute. The cytometer was evaluated by analysing human blood, and a four part differential leukocyte assay for accurate CD4+ T-cell enumeration was demonstrated. The integration of impedance, fluorescence and side scatter into a single miniature cytometer platform provides the core information content of a classical cytometer in a highly compact, simple, portable and low cost format.
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Accepted/In Press date: 5 June 2014
e-pub ahead of print date: 5 June 2014
Published date: 2014
Organisations:
Electronics & Computer Science
Identifiers
Local EPrints ID: 368167
URI: http://eprints.soton.ac.uk/id/eprint/368167
ISSN: 1473-0197
PURE UUID: 3a5f4742-b337-48a9-b566-d6493e01ec45
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Date deposited: 19 Aug 2014 12:33
Last modified: 15 Mar 2024 03:18
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Contributors
Author:
Daniel Spencer
Author:
Gregor Elliott
Author:
Hywel Morgan
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