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A sheath-less combined optical and impedance micro-cytometer

Spencer, Daniel, Elliott, Gregor and Morgan, Hywel (2014) A sheath-less combined optical and impedance micro-cytometer Lab on a Chip, 14, (16), pp. 3064-3073. (doi:10.1039/c4lc00224e).

Record type: Article

Abstract

We describe a sheath-less micro-cytometer that measures four different parameters, namely fluorescence, large angle side scatter and dual frequency electrical impedance (electrical volume and opacity). The cytometer was benchmarked using both size and fluorescent bead standards and demonstrates excellent size accuracy (CVs ? 2.1%), sensitivity and dynamic range (3.5 orders of magnitude) at sample flow rates of 80 ?L per minute. The cytometer was evaluated by analysing human blood, and a four part differential leukocyte assay for accurate CD4+ T-cell enumeration was demonstrated. The integration of impedance, fluorescence and side scatter into a single miniature cytometer platform provides the core information content of a classical cytometer in a highly compact, simple, portable and low cost format.

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More information

Accepted/In Press date: 5 June 2014
Published date: 2014
Organisations: Electronics & Computer Science

Identifiers

Local EPrints ID: 368167
URI: http://eprints.soton.ac.uk/id/eprint/368167
ISSN: 1473-0197
PURE UUID: 3a5f4742-b337-48a9-b566-d6493e01ec45
ORCID for Hywel Morgan: ORCID iD orcid.org/0000-0003-4850-5676

Catalogue record

Date deposited: 19 Aug 2014 12:33
Last modified: 18 Jul 2017 01:50

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Contributors

Author: Daniel Spencer
Author: Gregor Elliott
Author: Hywel Morgan ORCID iD

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