A sheath-less combined optical and impedance micro-cytometer

Spencer, Daniel, Elliott, Gregor and Morgan, Hywel (2014) A sheath-less combined optical and impedance micro-cytometer Lab on a Chip, 14, (16), pp. 3064-3073. (doi:10.1039/c4lc00224e).


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We describe a sheath-less micro-cytometer that measures four different parameters, namely fluorescence, large angle side scatter and dual frequency electrical impedance (electrical volume and opacity). The cytometer was benchmarked using both size and fluorescent bead standards and demonstrates excellent size accuracy (CVs ? 2.1%), sensitivity and dynamic range (3.5 orders of magnitude) at sample flow rates of 80 ?L per minute. The cytometer was evaluated by analysing human blood, and a four part differential leukocyte assay for accurate CD4+ T-cell enumeration was demonstrated. The integration of impedance, fluorescence and side scatter into a single miniature cytometer platform provides the core information content of a classical cytometer in a highly compact, simple, portable and low cost format.

Item Type: Article
Digital Object Identifier (DOI): doi:10.1039/c4lc00224e
ISSNs: 1473-0197 (print)
Subjects: Q Science > QC Physics
Organisations: Electronics & Computer Science
ePrint ID: 368167
Date :
Date Event
5 June 2014Accepted/In Press
5 June 2014e-pub ahead of print
Date Deposited: 19 Aug 2014 12:33
Last Modified: 19 Jun 2017 16:32
Further Information:Google Scholar
URI: http://eprints.soton.ac.uk/id/eprint/368167

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