The University of Southampton
University of Southampton Institutional Repository

Sertoli cells control peritubular myoid cell fate and support adult Leydig cell development in the prepubertal testis

Sertoli cells control peritubular myoid cell fate and support adult Leydig cell development in the prepubertal testis
Sertoli cells control peritubular myoid cell fate and support adult Leydig cell development in the prepubertal testis
Sertoli cells (SCs) regulate testicular fate in the differentiating gonad and are the main regulators of spermatogenesis in the adult testis; however, their role during the intervening period of testis development, in particular during adult Leydig cell (ALC) differentiation and function, remains largely unknown. To examine SC function during fetal and prepubertal development we generated two transgenic mouse models that permit controlled, cell-specific ablation of SCs in pre- and postnatal life. Results show that SCs are required: (1) to maintain the differentiated phenotype of peritubular myoid cells (PTMCs) in prepubertal life; (2) to maintain the ALC progenitor population in the postnatal testis; and (3) for development of normal ALC numbers. Furthermore, our data show that fetal LCs function independently from SC, germ cell or PTMC support in the prepubertal testis. Together, these findings reveal that SCs remain essential regulators of testis development long after the period of sex determination. These findings have significant implications for our understanding of male reproductive disorders and wider androgen-related conditions affecting male health.
1477-9129
Rebourcet, Diane
743169a1-5ff6-42cf-abf5-e5ac7e88cbf1
O’Shaughnessy, Peter J.
95d85839-9855-48bd-bf14-e3285a6f0019
Pitetti, Jean-Luc
948bcddd-5a71-4c86-ac63-c373d073e5a3
Monteiro, Ana
018e5ec0-23b6-4c8d-b445-2e7a2852ed54
O’Hara, Laura
def2e6de-0565-42c0-8507-14818d405a00
Milne, Laura
562d4fd4-4873-4adb-a130-c5ca3632a8a2
Tsai, Ji Ting
11b8ed74-326f-4807-a5a2-15843ee32c2d
Cruickshanks, Lindsey
479ead9e-88e4-439b-8c56-7b910179d53e
Riethmacher, Dieter
1a0a0c2e-e94d-4d0a-a890-90107a2545bc
Guillou, Florian
81ac4282-1b5b-4ef0-a893-a882190a7c1e
Mitchell, Rod T.
e2420e43-5cee-491e-8a74-45e924230fb1
van ’t Hof, Rob
6365d3cd-9423-4915-b4b8-04049e6d794a
Freeman, Tom C.
e500260d-7ac7-48df-bf05-00b89a60fdf0
Nef, Serge
5cabb7cf-7965-4895-a0d1-02bd2dc3196a
Smith, Lee B.
8b6811ae-a03c-447b-b6c8-5ac060a88404
Rebourcet, Diane
743169a1-5ff6-42cf-abf5-e5ac7e88cbf1
O’Shaughnessy, Peter J.
95d85839-9855-48bd-bf14-e3285a6f0019
Pitetti, Jean-Luc
948bcddd-5a71-4c86-ac63-c373d073e5a3
Monteiro, Ana
018e5ec0-23b6-4c8d-b445-2e7a2852ed54
O’Hara, Laura
def2e6de-0565-42c0-8507-14818d405a00
Milne, Laura
562d4fd4-4873-4adb-a130-c5ca3632a8a2
Tsai, Ji Ting
11b8ed74-326f-4807-a5a2-15843ee32c2d
Cruickshanks, Lindsey
479ead9e-88e4-439b-8c56-7b910179d53e
Riethmacher, Dieter
1a0a0c2e-e94d-4d0a-a890-90107a2545bc
Guillou, Florian
81ac4282-1b5b-4ef0-a893-a882190a7c1e
Mitchell, Rod T.
e2420e43-5cee-491e-8a74-45e924230fb1
van ’t Hof, Rob
6365d3cd-9423-4915-b4b8-04049e6d794a
Freeman, Tom C.
e500260d-7ac7-48df-bf05-00b89a60fdf0
Nef, Serge
5cabb7cf-7965-4895-a0d1-02bd2dc3196a
Smith, Lee B.
8b6811ae-a03c-447b-b6c8-5ac060a88404

Rebourcet, Diane, O’Shaughnessy, Peter J., Pitetti, Jean-Luc, Monteiro, Ana, O’Hara, Laura, Milne, Laura, Tsai, Ji Ting, Cruickshanks, Lindsey, Riethmacher, Dieter, Guillou, Florian, Mitchell, Rod T., van ’t Hof, Rob, Freeman, Tom C., Nef, Serge and Smith, Lee B. (2014) Sertoli cells control peritubular myoid cell fate and support adult Leydig cell development in the prepubertal testis. Development, 141 (10). (doi:10.1242/dev.107029). (PMID:24803659)

Record type: Article

Abstract

Sertoli cells (SCs) regulate testicular fate in the differentiating gonad and are the main regulators of spermatogenesis in the adult testis; however, their role during the intervening period of testis development, in particular during adult Leydig cell (ALC) differentiation and function, remains largely unknown. To examine SC function during fetal and prepubertal development we generated two transgenic mouse models that permit controlled, cell-specific ablation of SCs in pre- and postnatal life. Results show that SCs are required: (1) to maintain the differentiated phenotype of peritubular myoid cells (PTMCs) in prepubertal life; (2) to maintain the ALC progenitor population in the postnatal testis; and (3) for development of normal ALC numbers. Furthermore, our data show that fetal LCs function independently from SC, germ cell or PTMC support in the prepubertal testis. Together, these findings reveal that SCs remain essential regulators of testis development long after the period of sex determination. These findings have significant implications for our understanding of male reproductive disorders and wider androgen-related conditions affecting male health.

Text
Development-2014-Rebourcet-2139-49.pdf - Version of Record
Restricted to Registered users only
Download (7MB)
Request a copy

More information

Submitted date: December 2013
Accepted/In Press date: 24 March 2014
e-pub ahead of print date: 6 May 2014
Published date: May 2014
Organisations: Faculty of Medicine

Identifiers

Local EPrints ID: 368183
URI: http://eprints.soton.ac.uk/id/eprint/368183
ISSN: 1477-9129
PURE UUID: 3fe4ad22-6715-497c-8f01-ece4739ccc16
ORCID for Dieter Riethmacher: ORCID iD orcid.org/0000-0002-4206-5529

Catalogue record

Date deposited: 20 Aug 2014 11:35
Last modified: 17 Dec 2019 01:43

Export record

Altmetrics

Contributors

Author: Diane Rebourcet
Author: Peter J. O’Shaughnessy
Author: Jean-Luc Pitetti
Author: Ana Monteiro
Author: Laura O’Hara
Author: Laura Milne
Author: Ji Ting Tsai
Author: Lindsey Cruickshanks
Author: Dieter Riethmacher ORCID iD
Author: Florian Guillou
Author: Rod T. Mitchell
Author: Rob van ’t Hof
Author: Tom C. Freeman
Author: Serge Nef
Author: Lee B. Smith

University divisions

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×