Free-standing nanostructured gold films with milled nanopores for DNA analysis
Free-standing nanostructured gold films with milled nanopores for DNA analysis
Nanoporous thin films are of much interest for emerging DNA sequencing applications [1]. Klarite® pyramidal nanostructured substrates and gold microcavities have demonstrated strong field enhancement within their structure [2,3]. Our strategy has been to develop these three-dimensional structures in gold film form. As displayed in figure 1, free-standing thin films (100 nm thick) of arrays of nano-sized gold pyramids and microcavities have been successfully fabricated. Precisely engineered nanopores in the three-dimensional nanostructured free-standing gold film are milled at specific locations using a helium ion microscope (HIM). These films are suspended over micron-sized apertures for optical interrogation within platforms already proven for DNA translocation and sequencing. The strong field enhancement at the base of structures is currently being investigated for enhancement of fluorescently labelled DNA strands passing through the nanopores.
We acknowledge the BBSRC (Ref: BB/I023720/1) and the CARIPLO Foundation, Italy for funding JAG-J and FC respectively and Renishaw Diagnostics, Glasgow for providing us with some of the samples used in fabricating these structures.
Grant-Jacob, J.
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Carpignano, F.
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Brocklesby, W.S.
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Melvin, T.
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September 2012
Grant-Jacob, J.
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Carpignano, F.
e794e71c-1adf-407d-aa80-5b302bc39864
Brocklesby, W.S.
c53ca2f6-db65-4e19-ad00-eebeb2e6de67
Melvin, T.
fd87f5eb-2bb9-48fa-b7be-7100ace9c50f
Grant-Jacob, J., Carpignano, F., Brocklesby, W.S. and Melvin, T.
(2012)
Free-standing nanostructured gold films with milled nanopores for DNA analysis.
IoP - Physics Meets Biology, Oxford, United Kingdom.
03 - 05 Sep 2012.
1 pp
.
Record type:
Conference or Workshop Item
(Paper)
Abstract
Nanoporous thin films are of much interest for emerging DNA sequencing applications [1]. Klarite® pyramidal nanostructured substrates and gold microcavities have demonstrated strong field enhancement within their structure [2,3]. Our strategy has been to develop these three-dimensional structures in gold film form. As displayed in figure 1, free-standing thin films (100 nm thick) of arrays of nano-sized gold pyramids and microcavities have been successfully fabricated. Precisely engineered nanopores in the three-dimensional nanostructured free-standing gold film are milled at specific locations using a helium ion microscope (HIM). These films are suspended over micron-sized apertures for optical interrogation within platforms already proven for DNA translocation and sequencing. The strong field enhancement at the base of structures is currently being investigated for enhancement of fluorescently labelled DNA strands passing through the nanopores.
We acknowledge the BBSRC (Ref: BB/I023720/1) and the CARIPLO Foundation, Italy for funding JAG-J and FC respectively and Renishaw Diagnostics, Glasgow for providing us with some of the samples used in fabricating these structures.
More information
Published date: September 2012
Venue - Dates:
IoP - Physics Meets Biology, Oxford, United Kingdom, 2012-09-03 - 2012-09-05
Organisations:
Optoelectronics Research Centre
Identifiers
Local EPrints ID: 368320
URI: http://eprints.soton.ac.uk/id/eprint/368320
PURE UUID: fa995844-5441-4d06-b694-2c10ce71914b
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Date deposited: 17 Sep 2014 13:59
Last modified: 15 Mar 2024 03:37
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Contributors
Author:
F. Carpignano
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