The University of Southampton
University of Southampton Institutional Repository

Lesion and stimulation of the ventral tegmental area increases cholinergic activity in the rat brain

Lesion and stimulation of the ventral tegmental area increases cholinergic activity in the rat brain
Lesion and stimulation of the ventral tegmental area increases cholinergic activity in the rat brain
Our previous study indicated that unilateral lesion of the ventral tegmental area (VTA) facilitates contralateral VTA stimulation-induced feeding or exploration. The present study was aimed to determine the possible role of the central cholinergic systems in this effect. Immunohistochemistry for choline acetyltransferase (ChAT) was used to measure the number of active cholinergic neurons in their major groups (Ch1-Ch6) and in striatal regions in rats subjected to unilateral electrocoagulation and contralateral VTA electrical stimulation (L/S group) in comparison to the unilaterally stimulated (S), unilaterally lesioned (L) and sham (Sh) groups. The study showed that unilateral VTA lesion increased (as compared to Sh group) the number of ChAT+ neurons in the Ch1-Ch3 and unilateral VTA stimulation increased the number in the Ch1 and the ventral pallidum only. The most sensitive to these changes in the mesolimbic system were cholinergic structures providing hippocampal afferentation. Surprisingly, there was no significant increase in the number of ChAT+ neurons in the L/S group. The obtained results did not confirm any evident influence of the cholinergic systems on the VTA lesion-induced facilitation of the behavioral response evoked by contralateral VTA stimulation.
cholinergic system, ventral tegmental area, immunohistochemistry, choline acetyltransferase, stimulation, lesion
0065-1400
28-39
Majkutewicz, Irena
71dde6b6-c65e-41ea-b30e-3ef004cefa95
Cecot, Tomasz
eb39253c-283b-4000-af47-5f898749079d
Jerzemowska, Grazyna
c7a9d18c-0a9c-49a6-96f9-0528bbb888d9
Trojniar, Weronika
02fe6d4b-0157-437f-9e51-ea34f8658457
Jaskulski, Michał
a2367cb9-53dd-4b21-8376-f1335f414dd6
Wrona, Danuta
e89efa49-a597-43e0-83ca-4bc621dcd03a
Majkutewicz, Irena
71dde6b6-c65e-41ea-b30e-3ef004cefa95
Cecot, Tomasz
eb39253c-283b-4000-af47-5f898749079d
Jerzemowska, Grazyna
c7a9d18c-0a9c-49a6-96f9-0528bbb888d9
Trojniar, Weronika
02fe6d4b-0157-437f-9e51-ea34f8658457
Jaskulski, Michał
a2367cb9-53dd-4b21-8376-f1335f414dd6
Wrona, Danuta
e89efa49-a597-43e0-83ca-4bc621dcd03a

Majkutewicz, Irena, Cecot, Tomasz, Jerzemowska, Grazyna, Trojniar, Weronika, Jaskulski, Michał and Wrona, Danuta (2010) Lesion and stimulation of the ventral tegmental area increases cholinergic activity in the rat brain. Acta Neurobiologiae Experimentalis, 70 (1), 28-39. (PMID:20407484)

Record type: Article

Abstract

Our previous study indicated that unilateral lesion of the ventral tegmental area (VTA) facilitates contralateral VTA stimulation-induced feeding or exploration. The present study was aimed to determine the possible role of the central cholinergic systems in this effect. Immunohistochemistry for choline acetyltransferase (ChAT) was used to measure the number of active cholinergic neurons in their major groups (Ch1-Ch6) and in striatal regions in rats subjected to unilateral electrocoagulation and contralateral VTA electrical stimulation (L/S group) in comparison to the unilaterally stimulated (S), unilaterally lesioned (L) and sham (Sh) groups. The study showed that unilateral VTA lesion increased (as compared to Sh group) the number of ChAT+ neurons in the Ch1-Ch3 and unilateral VTA stimulation increased the number in the Ch1 and the ventral pallidum only. The most sensitive to these changes in the mesolimbic system were cholinergic structures providing hippocampal afferentation. Surprisingly, there was no significant increase in the number of ChAT+ neurons in the L/S group. The obtained results did not confirm any evident influence of the cholinergic systems on the VTA lesion-induced facilitation of the behavioral response evoked by contralateral VTA stimulation.

Full text not available from this repository.

More information

Published date: 2010
Keywords: cholinergic system, ventral tegmental area, immunohistochemistry, choline acetyltransferase, stimulation, lesion
Organisations: Faculty of Medicine

Identifiers

Local EPrints ID: 368552
URI: http://eprints.soton.ac.uk/id/eprint/368552
ISSN: 0065-1400
PURE UUID: c38ce734-a4e1-4de4-9637-8e67295acdf4
ORCID for Tomasz Cecot: ORCID iD orcid.org/0000-0002-3944-654X

Catalogue record

Date deposited: 17 Sep 2014 15:20
Last modified: 15 Jul 2019 21:46

Export record

Contributors

Author: Irena Majkutewicz
Author: Tomasz Cecot ORCID iD
Author: Grazyna Jerzemowska
Author: Weronika Trojniar
Author: Michał Jaskulski
Author: Danuta Wrona

University divisions

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×