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Changes in NGF/c-Fos double staining in the structures of the limbic system in juvenile and aged rats exposed to forced swim test

Changes in NGF/c-Fos double staining in the structures of the limbic system in juvenile and aged rats exposed to forced swim test
Changes in NGF/c-Fos double staining in the structures of the limbic system in juvenile and aged rats exposed to forced swim test
This study aimed to investigate the influence of acute (a single 15 min) and chronic (15 min daily for 21 days) exposure to forced swim (FS) test on nerve growth factor (NGF)/c-Fos cells in hypothalamic paraventricular (PV) and supraoptic (SO) nuclei, the central (CeA) and medial (MeA) amygdaloid nuclei and CA3-hippocampus in juvenile (P28) and aged (P360) rats. The double-immunofluorescence (-ir) method was used to detect NGF-ir and c-Fos-ir cells. The amount of NGF/c-Fosir cells in relation to all NGF-ir cells is shown as a percentage. In the acute FS test an increase in NGF/c-Fos-ir cells (P<.05) was observed in all studied structures of juvenile rats and in the PV and SO of the aged individuals. After chronic FS stress, the NGF/c-Fos-ir ratio remained unaltered (except in the SO) in P28, but it increased (P<.05) in all investigated regions in P360 compared with the controls. The findings may reflect the state of molecular plasticity within the limbic hypothalamicpituitary- adrenocortical (HPA) axis in both age groups, yet the phenomenon of habituation in NGF/c-Fos-ir after chronic FS exposure was observed only in juvenile animals.
0065-1400
448-458
Badowska-Szalewska, Ewa
9348a276-5395-4744-a3fb-175ff13559bf
Klejbor, Ilona
0458d1c4-b819-4ced-ac73-3111bfd96304
Cecot, Tomasz
eb39253c-283b-4000-af47-5f898749079d
Spodnik, Jan H.
58048c6b-5065-494e-a14c-83b0fb427d7a
Moryś, Janusz
da472c35-18d4-465a-b167-bf5e2d15ac3a
Badowska-Szalewska, Ewa
9348a276-5395-4744-a3fb-175ff13559bf
Klejbor, Ilona
0458d1c4-b819-4ced-ac73-3111bfd96304
Cecot, Tomasz
eb39253c-283b-4000-af47-5f898749079d
Spodnik, Jan H.
58048c6b-5065-494e-a14c-83b0fb427d7a
Moryś, Janusz
da472c35-18d4-465a-b167-bf5e2d15ac3a

Badowska-Szalewska, Ewa, Klejbor, Ilona, Cecot, Tomasz, Spodnik, Jan H. and Moryś, Janusz (2009) Changes in NGF/c-Fos double staining in the structures of the limbic system in juvenile and aged rats exposed to forced swim test. Acta Neurobiologiae Experimentalis, 69 (4), 448-458. (PMID:20048762)

Record type: Article

Abstract

This study aimed to investigate the influence of acute (a single 15 min) and chronic (15 min daily for 21 days) exposure to forced swim (FS) test on nerve growth factor (NGF)/c-Fos cells in hypothalamic paraventricular (PV) and supraoptic (SO) nuclei, the central (CeA) and medial (MeA) amygdaloid nuclei and CA3-hippocampus in juvenile (P28) and aged (P360) rats. The double-immunofluorescence (-ir) method was used to detect NGF-ir and c-Fos-ir cells. The amount of NGF/c-Fosir cells in relation to all NGF-ir cells is shown as a percentage. In the acute FS test an increase in NGF/c-Fos-ir cells (P<.05) was observed in all studied structures of juvenile rats and in the PV and SO of the aged individuals. After chronic FS stress, the NGF/c-Fos-ir ratio remained unaltered (except in the SO) in P28, but it increased (P<.05) in all investigated regions in P360 compared with the controls. The findings may reflect the state of molecular plasticity within the limbic hypothalamicpituitary- adrenocortical (HPA) axis in both age groups, yet the phenomenon of habituation in NGF/c-Fos-ir after chronic FS exposure was observed only in juvenile animals.

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linkout.php_pii=6933 - Accepted Manuscript
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Published date: 2009
Organisations: Faculty of Medicine

Identifiers

Local EPrints ID: 368556
URI: https://eprints.soton.ac.uk/id/eprint/368556
ISSN: 0065-1400
PURE UUID: 84be1e0f-97d2-4e49-b691-e9daf689e5f2
ORCID for Tomasz Cecot: ORCID iD orcid.org/0000-0002-3944-654X

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Date deposited: 22 Sep 2014 12:11
Last modified: 18 Jul 2017 01:45

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Contributors

Author: Ewa Badowska-Szalewska
Author: Ilona Klejbor
Author: Tomasz Cecot ORCID iD
Author: Jan H. Spodnik
Author: Janusz Moryś

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