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Antibody repertoire against the A60 antigen complex during the course of pulmonary tuberculosis

Antibody repertoire against the A60 antigen complex during the course of pulmonary tuberculosis
Antibody repertoire against the A60 antigen complex during the course of pulmonary tuberculosis
The A60 antigen complex is a Mycobacterium bovis (BCG) highly immunodominant antigen containing both B and T-cell epitopes. Clinical-serological studies show that elevated anti-A60 titres are present during tuberculosis. We wished to analyze in detail antibody responses against A60 components during the course of tuberculosis. A mixed longitudinal study was designed including individuals at the onset of tuberculosis, during treatment and after resolution of the disease. The anti-A60 repertoire was analyzed using a western blot assay with A60 as the antigen. While PPD- normals recognized only the 65 kDa heat shock protein (HSP), PPD+ normal individuals displayed low levels of anti-A60 antibodies against dominant antigens. There were immunoglobulin M (IgM) and immunoglobulin G (IgG) consistent with response to a latent infection. Onset tuberculosis was characterized by IgM and IgG antibodies against 52 to 28 kDa antigens; IgM response being limited to earlier phases of the disease. In contrast, IgM antibodies against 25 to 14 kDa antigens appeared only 2-6 months after disease onset. The antibody repertoire of chemotherapy-treated, resolved tuberculosis was exclusively IgG in isotype, as for a memory-type response. Thus, western blot analysis with A60 identifies typical antibody patterns associated with different clinical phases of tuberculosis infection. Such approach may help in identifying new single antigens for serologic diagnosis of active tuberculosis.
A60 antigen, antibody repertoire, tuberculosis
0903-1936
816-822
Amicosante, M.
deeb6cc8-e796-4c25-8e92-6b79000d737c
Paone, G.
c98dba89-5edb-40e2-8efa-fd5246050f35
Ameglio, F.
fe671f70-3e6c-46eb-9dc3-4b78d55f6af6
Bianchi, E. L.
995a34ac-87c6-409f-9343-30c2db388862
Piccolella, E.
801a33c8-4bb5-4b3f-9e89-85eadbcb39ef
Richeldi, L.
47177d9c-731a-49a1-9cc6-4ac8f6bbbf26
Bisetti, A.
4fe0ef63-f59f-4a11-ac4f-24598087a3b2
Luisetti, M.
b0adf46a-ad71-4678-8ab8-3fb97050982a
Saltini, C.
511217a8-2901-4ca3-bbdf-b54611e4acc2
Amicosante, M.
deeb6cc8-e796-4c25-8e92-6b79000d737c
Paone, G.
c98dba89-5edb-40e2-8efa-fd5246050f35
Ameglio, F.
fe671f70-3e6c-46eb-9dc3-4b78d55f6af6
Bianchi, E. L.
995a34ac-87c6-409f-9343-30c2db388862
Piccolella, E.
801a33c8-4bb5-4b3f-9e89-85eadbcb39ef
Richeldi, L.
47177d9c-731a-49a1-9cc6-4ac8f6bbbf26
Bisetti, A.
4fe0ef63-f59f-4a11-ac4f-24598087a3b2
Luisetti, M.
b0adf46a-ad71-4678-8ab8-3fb97050982a
Saltini, C.
511217a8-2901-4ca3-bbdf-b54611e4acc2

Amicosante, M., Paone, G., Ameglio, F., Bianchi, E. L., Piccolella, E., Richeldi, L., Bisetti, A., Luisetti, M. and Saltini, C. (1993) Antibody repertoire against the A60 antigen complex during the course of pulmonary tuberculosis. European Respiratory Journal, 6 (6), 816-822. (PMID:8339800)

Record type: Article

Abstract

The A60 antigen complex is a Mycobacterium bovis (BCG) highly immunodominant antigen containing both B and T-cell epitopes. Clinical-serological studies show that elevated anti-A60 titres are present during tuberculosis. We wished to analyze in detail antibody responses against A60 components during the course of tuberculosis. A mixed longitudinal study was designed including individuals at the onset of tuberculosis, during treatment and after resolution of the disease. The anti-A60 repertoire was analyzed using a western blot assay with A60 as the antigen. While PPD- normals recognized only the 65 kDa heat shock protein (HSP), PPD+ normal individuals displayed low levels of anti-A60 antibodies against dominant antigens. There were immunoglobulin M (IgM) and immunoglobulin G (IgG) consistent with response to a latent infection. Onset tuberculosis was characterized by IgM and IgG antibodies against 52 to 28 kDa antigens; IgM response being limited to earlier phases of the disease. In contrast, IgM antibodies against 25 to 14 kDa antigens appeared only 2-6 months after disease onset. The antibody repertoire of chemotherapy-treated, resolved tuberculosis was exclusively IgG in isotype, as for a memory-type response. Thus, western blot analysis with A60 identifies typical antibody patterns associated with different clinical phases of tuberculosis infection. Such approach may help in identifying new single antigens for serologic diagnosis of active tuberculosis.

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More information

Published date: 1 June 1993
Keywords: A60 antigen, antibody repertoire, tuberculosis
Organisations: Clinical & Experimental Sciences

Identifiers

Local EPrints ID: 368932
URI: http://eprints.soton.ac.uk/id/eprint/368932
ISSN: 0903-1936
PURE UUID: 8e723edc-e2fe-4c87-867a-f46008ed6f7b

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Date deposited: 18 Sep 2014 14:04
Last modified: 18 Jul 2017 01:41

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Contributors

Author: M. Amicosante
Author: G. Paone
Author: F. Ameglio
Author: E. L. Bianchi
Author: E. Piccolella
Author: L. Richeldi
Author: A. Bisetti
Author: M. Luisetti
Author: C. Saltini

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