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Inactivation of polymerase inhibitors for Mycobacterium tuberculosis DNA amplification in sputum by using capture resin

Inactivation of polymerase inhibitors for Mycobacterium tuberculosis DNA amplification in sputum by using capture resin
Inactivation of polymerase inhibitors for Mycobacterium tuberculosis DNA amplification in sputum by using capture resin
Endogenous PCR inhibitors hamper DNA amplification of Mycobacterium tuberculosis DNA in sputum samples. Use of an anion binding resin (GeneReleaser [GR]) improved PCR sensitivity from 77% (27 of 35 culture-positive samples) with phenol-chloroform extraction (P-C) alone to 91% (GR without P-C; P > 0.05) and 100% (P-C and GR combined; P < 0.05).
0095-1137
629-630
Amicosante, M.
deeb6cc8-e796-4c25-8e92-6b79000d737c
Richeldi, L.
47177d9c-731a-49a1-9cc6-4ac8f6bbbf26
Trenti, G.
8df4c793-d297-47ef-81aa-8476c5e2bedb
Paone, G.
c98dba89-5edb-40e2-8efa-fd5246050f35
Campa, M.
6d9b223a-abbf-4edb-bf89-31a731f8b00e
Bisetti, A.
4fe0ef63-f59f-4a11-ac4f-24598087a3b2
Saltini, C.
511217a8-2901-4ca3-bbdf-b54611e4acc2
Amicosante, M.
deeb6cc8-e796-4c25-8e92-6b79000d737c
Richeldi, L.
47177d9c-731a-49a1-9cc6-4ac8f6bbbf26
Trenti, G.
8df4c793-d297-47ef-81aa-8476c5e2bedb
Paone, G.
c98dba89-5edb-40e2-8efa-fd5246050f35
Campa, M.
6d9b223a-abbf-4edb-bf89-31a731f8b00e
Bisetti, A.
4fe0ef63-f59f-4a11-ac4f-24598087a3b2
Saltini, C.
511217a8-2901-4ca3-bbdf-b54611e4acc2

Amicosante, M., Richeldi, L., Trenti, G., Paone, G., Campa, M., Bisetti, A. and Saltini, C. (1995) Inactivation of polymerase inhibitors for Mycobacterium tuberculosis DNA amplification in sputum by using capture resin. Journal of Clinical Microbiology, 33 (3), 629-630. (PMID:7751368)

Record type: Article

Abstract

Endogenous PCR inhibitors hamper DNA amplification of Mycobacterium tuberculosis DNA in sputum samples. Use of an anion binding resin (GeneReleaser [GR]) improved PCR sensitivity from 77% (27 of 35 culture-positive samples) with phenol-chloroform extraction (P-C) alone to 91% (GR without P-C; P > 0.05) and 100% (P-C and GR combined; P < 0.05).

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More information

Published date: March 1995
Organisations: Clinical & Experimental Sciences

Identifiers

Local EPrints ID: 368934
URI: http://eprints.soton.ac.uk/id/eprint/368934
ISSN: 0095-1137
PURE UUID: 2c91e6fb-2b05-4ec7-a211-8aa6bcdbf3ee

Catalogue record

Date deposited: 23 Sep 2014 11:28
Last modified: 18 Jul 2017 01:41

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Contributors

Author: M. Amicosante
Author: L. Richeldi
Author: G. Trenti
Author: G. Paone
Author: M. Campa
Author: A. Bisetti
Author: C. Saltini

University divisions

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