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Use of a T-cell interferon-gamma release assay for the diagnosis of tuberculous pleurisy

Use of a T-cell interferon-gamma release assay for the diagnosis of tuberculous pleurisy
Use of a T-cell interferon-gamma release assay for the diagnosis of tuberculous pleurisy
The diagnosis of pleural tuberculosis (plTB) by the analysis of pleural effusions (PEs) with standard diagnostic tools is difficult. In routine clinical practice, the present authors evaluated the performance of a commercially available Mycobacterium tuberculosis (MTB)-specific enzyme-linked immunospot assay on peripheral blood mononuclear cells (PBMCs) and pleural effusion mononuclear cells (PEMCs) in patients with suspect plTB.

The T-SPOT.TB test (Oxford Immunotec Ltd, Abingdon, UK) was performed on PBMCs and PEMCs in 20 patients with a clinical and radiological suspect of plTB and in 21 control subjects with a diagnosis of PE of nontuberculous origin at four centres participating in the European Tuberculosis Network.

In total, 18 (90%) out of 20 patients with plTB tested T-SPOT.TB-positive on PBMCs and 19 (95%) out of 20 on PEMCs. Among controls, T-SPOT.TB was positive in seven out of 21 (33%) patients when performed on PBMCs (these patients were assumed to be latently infected with MTB) and five (23%) out of 21 when performed on PEMCs. Sensitivity and specificity of T-SPOT.TB for the diagnosis of active plTB when performed on PEMCs were 95 and 76%, respectively.

Enumerating Mycobacterium tuberculosis-specific T-cells in pleural effusion mononuclear cells by ELISPOT is feasible in routine clinical practice and may be useful for a rapid and accurate diagnosis of pleural tuberculosis.
culture filtrate protein-10, early secretory antigenic target-6, pleurisy, T-cell interferon-? release assay, tuberculosis
0903-1936
1173-1179
Losi, M.
5d48476e-5760-47eb-bd74-573132186a06
Bossink, A.
1fabf294-9ed2-4478-aa47-2df7d4c3aaa5
Codecasa, L.
800411a2-98df-4380-b0d9-d6e99da600a1
Jafari, C.
7a4838c1-4596-4a57-b83a-feee6119ab75
Ernst, M.
577e5b64-aa72-47d1-8093-7b1f9a27dc76
Thijsen, S.
bb1fa824-5134-47a9-818e-641c7d69abe8
Cirillo, D.
392fb08e-4d14-4065-9e7a-492e898e4422
Ferrarese, M.
52697bfe-2094-45d2-9af0-89121dc21198
Greinert, U.
81a87c32-3405-4ff3-9f40-d6017f57e6a1
Fabbri, L.M.
bcd21e7a-25ed-458d-8cbf-500e45b79a1f
Richeldi, L.
47177d9c-731a-49a1-9cc6-4ac8f6bbbf26
Lange, C.
487625db-b62c-426d-a515-7d56202ee73d
Losi, M.
5d48476e-5760-47eb-bd74-573132186a06
Bossink, A.
1fabf294-9ed2-4478-aa47-2df7d4c3aaa5
Codecasa, L.
800411a2-98df-4380-b0d9-d6e99da600a1
Jafari, C.
7a4838c1-4596-4a57-b83a-feee6119ab75
Ernst, M.
577e5b64-aa72-47d1-8093-7b1f9a27dc76
Thijsen, S.
bb1fa824-5134-47a9-818e-641c7d69abe8
Cirillo, D.
392fb08e-4d14-4065-9e7a-492e898e4422
Ferrarese, M.
52697bfe-2094-45d2-9af0-89121dc21198
Greinert, U.
81a87c32-3405-4ff3-9f40-d6017f57e6a1
Fabbri, L.M.
bcd21e7a-25ed-458d-8cbf-500e45b79a1f
Richeldi, L.
47177d9c-731a-49a1-9cc6-4ac8f6bbbf26
Lange, C.
487625db-b62c-426d-a515-7d56202ee73d

Losi, M., Bossink, A., Codecasa, L., Jafari, C., Ernst, M., Thijsen, S., Cirillo, D., Ferrarese, M., Greinert, U., Fabbri, L.M., Richeldi, L. and Lange, C. (2007) Use of a T-cell interferon-gamma release assay for the diagnosis of tuberculous pleurisy. European Respiratory Journal, 30 (6), 1173-1179. (doi:10.1183/09031936.00067307). (PMID:17715165)

Record type: Article

Abstract

The diagnosis of pleural tuberculosis (plTB) by the analysis of pleural effusions (PEs) with standard diagnostic tools is difficult. In routine clinical practice, the present authors evaluated the performance of a commercially available Mycobacterium tuberculosis (MTB)-specific enzyme-linked immunospot assay on peripheral blood mononuclear cells (PBMCs) and pleural effusion mononuclear cells (PEMCs) in patients with suspect plTB.

The T-SPOT.TB test (Oxford Immunotec Ltd, Abingdon, UK) was performed on PBMCs and PEMCs in 20 patients with a clinical and radiological suspect of plTB and in 21 control subjects with a diagnosis of PE of nontuberculous origin at four centres participating in the European Tuberculosis Network.

In total, 18 (90%) out of 20 patients with plTB tested T-SPOT.TB-positive on PBMCs and 19 (95%) out of 20 on PEMCs. Among controls, T-SPOT.TB was positive in seven out of 21 (33%) patients when performed on PBMCs (these patients were assumed to be latently infected with MTB) and five (23%) out of 21 when performed on PEMCs. Sensitivity and specificity of T-SPOT.TB for the diagnosis of active plTB when performed on PEMCs were 95 and 76%, respectively.

Enumerating Mycobacterium tuberculosis-specific T-cells in pleural effusion mononuclear cells by ELISPOT is feasible in routine clinical practice and may be useful for a rapid and accurate diagnosis of pleural tuberculosis.

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More information

e-pub ahead of print date: 22 August 2007
Published date: 1 December 2007
Keywords: culture filtrate protein-10, early secretory antigenic target-6, pleurisy, T-cell interferon-? release assay, tuberculosis
Organisations: Clinical & Experimental Sciences

Identifiers

Local EPrints ID: 368971
URI: https://eprints.soton.ac.uk/id/eprint/368971
ISSN: 0903-1936
PURE UUID: e2fc403a-f6c7-4f40-850f-8aa3accbe7f3

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Date deposited: 10 Oct 2014 14:01
Last modified: 18 Jul 2017 01:41

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Contributors

Author: M. Losi
Author: A. Bossink
Author: L. Codecasa
Author: C. Jafari
Author: M. Ernst
Author: S. Thijsen
Author: D. Cirillo
Author: M. Ferrarese
Author: U. Greinert
Author: L.M. Fabbri
Author: L. Richeldi
Author: C. Lange

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