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Interferon-gamma-release assays detect recent tuberculosis re-infection in elderly contacts

Interferon-gamma-release assays detect recent tuberculosis re-infection in elderly contacts
Interferon-gamma-release assays detect recent tuberculosis re-infection in elderly contacts
The tuberculin skin test (TST) does not discriminate between recent and remote latent tuberculosis infection (LTBI). This study was carried out to test two interferon-gamma-based blood assays in recent contacts with high prevalence of remote LTBI. We performed a contact tracing investigation in a nursing home for the elderly, where elderly patients were exposed to a case of pulmonary tuberculosis. TST, QuantiFERON-TB Gold (QFT-G) and T-SPOT.TB (TS.TB) were performed 8 weeks after the end of potential exposure. IFN-gamma measurements were recorded and correlation with exposure was evaluated. Twenty-seven (37.5%), 32 (44.4%) and 16 (22.2%) subjects were TST, TS.TB and QFT-G positive, respectively; agreement between TS.TB and QFT-G was good among exposed subjects only (K=0.915, 0.218 in unexposed, p<0.001). When amounts of IFN-gamma were corrected for the number of producing T cells, specific IFN-gamma production was significantly different between exposed and unexposed individuals (16.75+/-5.40 vs 2.33+/-0.71 IFN-gamma IU/1000 SFC, p=0.0001). QFT-G and TS.TB provided discordant results among elderly contacts. Unlike TST, the specific IFN-gamma response might discriminate between recent and long-lasting tuberculosis infection.
0394-6320
669-677
Ferrara, G.
a37c56e2-3b94-443b-ae64-15f9548fb340
Losi, M.
5d48476e-5760-47eb-bd74-573132186a06
D'Amico, R.
088b30d2-395e-4613-8766-e728de5eeb23
Cagarelli, R.
d7d3a62c-246d-450b-afca-13fc2d732999
Pezzi, A.M.
a0d51751-1116-4ded-af58-39353bedd8d4
Meacci, M.
ee7c7cab-30bf-4588-9fe6-eccbd9db7934
Meccugni, B.
e36eca41-547d-44e9-8bf1-e9ec889ff40f
Marchetti Dori, I.
e01a9183-88d1-452e-a9fe-b695c312717d
Rumpianesi, F.
ff04419f-0660-4845-bd4c-bf81fc66d760
Roversi, P.
5645a826-2059-4add-b0f4-c1f761fe2e11
Casali, L.
a65e0782-6a9b-4680-86d2-ee0215a0b20c
Fabbri, L.M.
bcd21e7a-25ed-458d-8cbf-500e45b79a1f
Richeldi, L.
47177d9c-731a-49a1-9cc6-4ac8f6bbbf26
Ferrara, G.
a37c56e2-3b94-443b-ae64-15f9548fb340
Losi, M.
5d48476e-5760-47eb-bd74-573132186a06
D'Amico, R.
088b30d2-395e-4613-8766-e728de5eeb23
Cagarelli, R.
d7d3a62c-246d-450b-afca-13fc2d732999
Pezzi, A.M.
a0d51751-1116-4ded-af58-39353bedd8d4
Meacci, M.
ee7c7cab-30bf-4588-9fe6-eccbd9db7934
Meccugni, B.
e36eca41-547d-44e9-8bf1-e9ec889ff40f
Marchetti Dori, I.
e01a9183-88d1-452e-a9fe-b695c312717d
Rumpianesi, F.
ff04419f-0660-4845-bd4c-bf81fc66d760
Roversi, P.
5645a826-2059-4add-b0f4-c1f761fe2e11
Casali, L.
a65e0782-6a9b-4680-86d2-ee0215a0b20c
Fabbri, L.M.
bcd21e7a-25ed-458d-8cbf-500e45b79a1f
Richeldi, L.
47177d9c-731a-49a1-9cc6-4ac8f6bbbf26

Ferrara, G., Losi, M., D'Amico, R., Cagarelli, R., Pezzi, A.M., Meacci, M., Meccugni, B., Marchetti Dori, I., Rumpianesi, F., Roversi, P., Casali, L., Fabbri, L.M. and Richeldi, L. (2009) Interferon-gamma-release assays detect recent tuberculosis re-infection in elderly contacts. International Journal of Immunopathology and Pharmacology, 22 (3), 669-677. (PMID:19822083)

Record type: Article

Abstract

The tuberculin skin test (TST) does not discriminate between recent and remote latent tuberculosis infection (LTBI). This study was carried out to test two interferon-gamma-based blood assays in recent contacts with high prevalence of remote LTBI. We performed a contact tracing investigation in a nursing home for the elderly, where elderly patients were exposed to a case of pulmonary tuberculosis. TST, QuantiFERON-TB Gold (QFT-G) and T-SPOT.TB (TS.TB) were performed 8 weeks after the end of potential exposure. IFN-gamma measurements were recorded and correlation with exposure was evaluated. Twenty-seven (37.5%), 32 (44.4%) and 16 (22.2%) subjects were TST, TS.TB and QFT-G positive, respectively; agreement between TS.TB and QFT-G was good among exposed subjects only (K=0.915, 0.218 in unexposed, p<0.001). When amounts of IFN-gamma were corrected for the number of producing T cells, specific IFN-gamma production was significantly different between exposed and unexposed individuals (16.75+/-5.40 vs 2.33+/-0.71 IFN-gamma IU/1000 SFC, p=0.0001). QFT-G and TS.TB provided discordant results among elderly contacts. Unlike TST, the specific IFN-gamma response might discriminate between recent and long-lasting tuberculosis infection.

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More information

Published date: July 2009
Organisations: Clinical & Experimental Sciences

Identifiers

Local EPrints ID: 369023
URI: http://eprints.soton.ac.uk/id/eprint/369023
ISSN: 0394-6320
PURE UUID: 7f4d72b2-1319-4a42-a593-eec947bde055

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Date deposited: 23 Sep 2014 13:14
Last modified: 22 Jul 2022 19:08

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Contributors

Author: G. Ferrara
Author: M. Losi
Author: R. D'Amico
Author: R. Cagarelli
Author: A.M. Pezzi
Author: M. Meacci
Author: B. Meccugni
Author: I. Marchetti Dori
Author: F. Rumpianesi
Author: P. Roversi
Author: L. Casali
Author: L.M. Fabbri
Author: L. Richeldi

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