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[Mycobacterium tuberculosis. From the gene to the diagnosis]

[Mycobacterium tuberculosis. From the gene to the diagnosis]
[Mycobacterium tuberculosis. From the gene to the diagnosis]
Pulmonary tuberculosis still in on the list of the world major health problems. Tuberculosis has not been eradicated yet, from developing countries. Furthermore, its incidence is increasing in the industrialized world, due to the human immunodeficiency virus (HIV) epidemic. In this regard, atypical clinical presentation of tuberculosis in individuals who have a deficient immune system, such as those at risk of tuberculosis because of HIV infection, makes the diagnostic process more difficult. Tuberculosis cases are often diagnosed later in HIV individuals compared to non-HIV individuals. The ensuing greater risk of contagion thus requires rapid and sensitive diagnostic protocols. In this context, several biotechnological tools have been developed that can be applied to the diagnosis of tuberculosis. M. tuberculosis genes have been cloned, monoclonal antibodies against pure proteins have been produced, thus enabling researchers to generate molecular and biochemical probes. As a consequence, DNA hybridization and DNA amplification techniques have been applied to the detection of mycobacteria, and ELISA kits of high sensitivity are been already made available. In regard to the latter, it is likely that monospecific and highly sensitive immunoassays will be developed that are directed against "active disease" immunodominant antigens. It may thus be expected that future new technologies will supplement the traditional tools for the diagnosis of tuberculosis and rapid diagnosis protocols will be available to chest clinicians in a foreseeable future.
0034-1193
38-45
Saltini, C.
511217a8-2901-4ca3-bbdf-b54611e4acc2
Amicosante, M.
deeb6cc8-e796-4c25-8e92-6b79000d737c
Li Bianchi, E.
38e2cea4-f41b-4658-b32f-833509cf510f
Paone, G.
c98dba89-5edb-40e2-8efa-fd5246050f35
Richeldi, L.
47177d9c-731a-49a1-9cc6-4ac8f6bbbf26
Guerritore, D.
e884627e-7fea-45da-ad46-0326a7350e5a
Pallotta, G.
d102d1b4-73cb-423b-9d63-032a6a164161
Bisetti, A.
4fe0ef63-f59f-4a11-ac4f-24598087a3b2
Saltini, C.
511217a8-2901-4ca3-bbdf-b54611e4acc2
Amicosante, M.
deeb6cc8-e796-4c25-8e92-6b79000d737c
Li Bianchi, E.
38e2cea4-f41b-4658-b32f-833509cf510f
Paone, G.
c98dba89-5edb-40e2-8efa-fd5246050f35
Richeldi, L.
47177d9c-731a-49a1-9cc6-4ac8f6bbbf26
Guerritore, D.
e884627e-7fea-45da-ad46-0326a7350e5a
Pallotta, G.
d102d1b4-73cb-423b-9d63-032a6a164161
Bisetti, A.
4fe0ef63-f59f-4a11-ac4f-24598087a3b2

Saltini, C., Amicosante, M., Li Bianchi, E., Paone, G., Richeldi, L., Guerritore, D., Pallotta, G. and Bisetti, A. (1992) [Mycobacterium tuberculosis. From the gene to the diagnosis]. Recenti Progressi in Medicina, 83 (1), 38-45. (PMID:1561482)

Record type: Article

Abstract

Pulmonary tuberculosis still in on the list of the world major health problems. Tuberculosis has not been eradicated yet, from developing countries. Furthermore, its incidence is increasing in the industrialized world, due to the human immunodeficiency virus (HIV) epidemic. In this regard, atypical clinical presentation of tuberculosis in individuals who have a deficient immune system, such as those at risk of tuberculosis because of HIV infection, makes the diagnostic process more difficult. Tuberculosis cases are often diagnosed later in HIV individuals compared to non-HIV individuals. The ensuing greater risk of contagion thus requires rapid and sensitive diagnostic protocols. In this context, several biotechnological tools have been developed that can be applied to the diagnosis of tuberculosis. M. tuberculosis genes have been cloned, monoclonal antibodies against pure proteins have been produced, thus enabling researchers to generate molecular and biochemical probes. As a consequence, DNA hybridization and DNA amplification techniques have been applied to the detection of mycobacteria, and ELISA kits of high sensitivity are been already made available. In regard to the latter, it is likely that monospecific and highly sensitive immunoassays will be developed that are directed against "active disease" immunodominant antigens. It may thus be expected that future new technologies will supplement the traditional tools for the diagnosis of tuberculosis and rapid diagnosis protocols will be available to chest clinicians in a foreseeable future.

Full text not available from this repository.

More information

Published date: January 1992
Additional Information: Article in Italian
Organisations: Clinical & Experimental Sciences

Identifiers

Local EPrints ID: 369054
URI: https://eprints.soton.ac.uk/id/eprint/369054
ISSN: 0034-1193
PURE UUID: 0da3a026-e75f-4bb9-a7e7-92de34807aea

Catalogue record

Date deposited: 10 Oct 2014 14:09
Last modified: 18 Jul 2017 01:40

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