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A relationship between Pseudomonal growth behaviour and cystic fibrosis patient lung function identified in a metabolomic investigation

A relationship between Pseudomonal growth behaviour and cystic fibrosis patient lung function identified in a metabolomic investigation
A relationship between Pseudomonal growth behaviour and cystic fibrosis patient lung function identified in a metabolomic investigation
Chronic polymicrobial lung infections in adult cystic fibrosis patients are typically dominated by high levels of Pseudomonas aeruginosa. Determining the impact of P. aeruginosa growth on airway secretion composition is fundamental to understanding both the behaviour of this pathogen in vivo, and its relationship with other potential colonising species. We hypothesised that the marked differences in the phenotypes of clinical isolates would be reflected in the metabolite composition of spent culture media. 1H NMR spectroscopy was used to characterise the impact of P. aeruginosa growth on a synthetic medium as part of an in vitro CF lower airways model system. Comparisons of 15 CF clinical isolates were made and four distinct metabolomic clusters identified. Highly significant relationships between P. aeruginosa isolate cluster membership and both patient lung function (FEV1) and spent culture pH were identified. This link between clinical isolate growth behaviour and FEV1 indicates characterisation of P. aeruginosa growth may find application in predicting patient lung function while the significant divergence in metabolite production and consumption observed between CF clinical isolates suggests dominant isolate characteristics have the potential to play both a selective role in microbiota composition and influence pseudomonal behaviour in vivo.
NMR, cystic fibrosis, pseudomonal, lung function
1573-3882
1262-1273
Kozlowska, Justyna
31a30c59-0e5e-4b6c-b8e7-3d9abd73242c
Rivett, Damian W.
dd44e46c-42c9-49a1-8ae9-9e1cee01b957
Vermeer, Louic S.
631f1335-8312-424c-a747-cc159cb47bd7
Carroll, Mary P.
f7e407a5-f7fa-4efd-a7f1-8e6140b86f50
Bruce, Kenneth D.
09fa4b3f-2183-4e6e-933d-70a3c1b20b5d
James Mason, A.
9025ee94-d6c3-48de-a021-7a0c497c9161
Rogers, Geraint B.
bd90d82a-4150-4e01-a755-b81559586da2
Kozlowska, Justyna
31a30c59-0e5e-4b6c-b8e7-3d9abd73242c
Rivett, Damian W.
dd44e46c-42c9-49a1-8ae9-9e1cee01b957
Vermeer, Louic S.
631f1335-8312-424c-a747-cc159cb47bd7
Carroll, Mary P.
f7e407a5-f7fa-4efd-a7f1-8e6140b86f50
Bruce, Kenneth D.
09fa4b3f-2183-4e6e-933d-70a3c1b20b5d
James Mason, A.
9025ee94-d6c3-48de-a021-7a0c497c9161
Rogers, Geraint B.
bd90d82a-4150-4e01-a755-b81559586da2

Kozlowska, Justyna, Rivett, Damian W., Vermeer, Louic S., Carroll, Mary P., Bruce, Kenneth D., James Mason, A. and Rogers, Geraint B. (2013) A relationship between Pseudomonal growth behaviour and cystic fibrosis patient lung function identified in a metabolomic investigation. Metabolomics, 9 (6), 1262-1273. (doi:10.1007/s11306-013-0538-5).

Record type: Article

Abstract

Chronic polymicrobial lung infections in adult cystic fibrosis patients are typically dominated by high levels of Pseudomonas aeruginosa. Determining the impact of P. aeruginosa growth on airway secretion composition is fundamental to understanding both the behaviour of this pathogen in vivo, and its relationship with other potential colonising species. We hypothesised that the marked differences in the phenotypes of clinical isolates would be reflected in the metabolite composition of spent culture media. 1H NMR spectroscopy was used to characterise the impact of P. aeruginosa growth on a synthetic medium as part of an in vitro CF lower airways model system. Comparisons of 15 CF clinical isolates were made and four distinct metabolomic clusters identified. Highly significant relationships between P. aeruginosa isolate cluster membership and both patient lung function (FEV1) and spent culture pH were identified. This link between clinical isolate growth behaviour and FEV1 indicates characterisation of P. aeruginosa growth may find application in predicting patient lung function while the significant divergence in metabolite production and consumption observed between CF clinical isolates suggests dominant isolate characteristics have the potential to play both a selective role in microbiota composition and influence pseudomonal behaviour in vivo.

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Published date: 2013
Keywords: NMR, cystic fibrosis, pseudomonal, lung function
Organisations: Faculty of Medicine

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Local EPrints ID: 369360
URI: http://eprints.soton.ac.uk/id/eprint/369360
ISSN: 1573-3882
PURE UUID: e4ce74db-c023-4520-89ff-fe816e90220c

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Date deposited: 24 Sep 2014 13:17
Last modified: 02 Dec 2019 20:41

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Contributors

Author: Justyna Kozlowska
Author: Damian W. Rivett
Author: Louic S. Vermeer
Author: Mary P. Carroll
Author: Kenneth D. Bruce
Author: A. James Mason
Author: Geraint B. Rogers

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