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Combined ion conductance and fluorescence confocal microscopy for biological cell membrane transport studies

Combined ion conductance and fluorescence confocal microscopy for biological cell membrane transport studies
Combined ion conductance and fluorescence confocal microscopy for biological cell membrane transport studies
Optical visualization of nanoscale morphological changes taking place in living biological cells during such important processes as endo- and exocytosis is challenging due to the low refractive index of lipid membranes. In this paper we summarize and discuss advances in the powerful combination of two complementary live imaging techniques, ion conductance and fluorescence confocal microscopy, that allows cell membrane topography to be related with molecular-specific fluorescence at high spatial and temporal resolution. We demonstrate the feasibility of the use of ion conductance microscopy to image apical plasma membrane of mouse embryo trophoblast outgrowth cells at a resolution sufficient to depict single endocytic pits. This opens the possibility to study individual endocytic events in embryo trophoblast outgrowth cells where endocytosis plays a crucial role during early stages of embryo development.
2040-8986
1-11
Shevchuk, Andriy
8388aa54-775a-47cf-b18b-a8ecf75ae996
Novak, P.
f7fc4dca-6596-4293-a6c5-39e7f2e3dcaf
Velazquez, M.A.
3b67a037-eed3-4268-92f8-7a4b58473472
Fleming, T.P.
2abf761a-e5a1-4fa7-a2c8-12e32d5d4c03
Korchev, Y.E.
17e874d7-e6e8-463d-854a-e89421c0df90
Shevchuk, Andriy
8388aa54-775a-47cf-b18b-a8ecf75ae996
Novak, P.
f7fc4dca-6596-4293-a6c5-39e7f2e3dcaf
Velazquez, M.A.
3b67a037-eed3-4268-92f8-7a4b58473472
Fleming, T.P.
2abf761a-e5a1-4fa7-a2c8-12e32d5d4c03
Korchev, Y.E.
17e874d7-e6e8-463d-854a-e89421c0df90

Shevchuk, Andriy, Novak, P., Velazquez, M.A., Fleming, T.P. and Korchev, Y.E. (2013) Combined ion conductance and fluorescence confocal microscopy for biological cell membrane transport studies. Journal of Optics, 15 (9), 1-11. (doi:10.1088/2040-8978/15/9/094005).

Record type: Article

Abstract

Optical visualization of nanoscale morphological changes taking place in living biological cells during such important processes as endo- and exocytosis is challenging due to the low refractive index of lipid membranes. In this paper we summarize and discuss advances in the powerful combination of two complementary live imaging techniques, ion conductance and fluorescence confocal microscopy, that allows cell membrane topography to be related with molecular-specific fluorescence at high spatial and temporal resolution. We demonstrate the feasibility of the use of ion conductance microscopy to image apical plasma membrane of mouse embryo trophoblast outgrowth cells at a resolution sufficient to depict single endocytic pits. This opens the possibility to study individual endocytic events in embryo trophoblast outgrowth cells where endocytosis plays a crucial role during early stages of embryo development.

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More information

Published date: 10 September 2013
Organisations: Optoelectronics Research Centre, Centre for Biological Sciences

Identifiers

Local EPrints ID: 369442
URI: https://eprints.soton.ac.uk/id/eprint/369442
ISSN: 2040-8986
PURE UUID: c8e3c0e3-df15-4be0-93d0-041d4d6945f5

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Date deposited: 01 Oct 2014 16:43
Last modified: 01 Mar 2019 17:31

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Contributors

Author: Andriy Shevchuk
Author: P. Novak
Author: M.A. Velazquez
Author: T.P. Fleming
Author: Y.E. Korchev

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