The University of Southampton
University of Southampton Institutional Repository

In vivo oocyte developmental competence is reduced in lean but not in obese superovulated dairy cows after intraovarian administration of IGF1

In vivo oocyte developmental competence is reduced in lean but not in obese superovulated dairy cows after intraovarian administration of IGF1
In vivo oocyte developmental competence is reduced in lean but not in obese superovulated dairy cows after intraovarian administration of IGF1
The present study investigated the role of IGF1 in lactating lean and non-lactating obese dairy cows by injecting 1??g IGF1 into the ovaries prior to superovulation. This amount of IGF1 has been linked with pregnancy loss in women with the polycystic ovary syndrome (PCOS) and was associated with impaired bovine oocyte competence in vitro. Transcript abundance and protein expression of selected genes involved in apoptosis, glucose metabolism, and the IGF system were analyzed. Plasma concentrations of IGF1 and leptin, and IGF1 in uterine luminal fluid (ULF), were also measured. IGF1 treatment decreased embryo viability in lean cows to the levels observed in obese cows. Obese cows were not affected by IGF1 treatment and showed elevated levels of IGF1 (in both plasma and ULF) and leptin. Blastocysts from lean cows treated with IGF1 showed a higher abundance of SLC2A1 and IGFBP3 transcripts. IGF1 treatment reduced protein expression of tumor protein 53 in blastocysts of lean cows, whereas the opposite was observed in obese cows. IGF1 in plasma and ULF was correlated only in the control groups. Blastocyst transcript abundance of IGF1 receptor and IGFBP3 correlated positively with IGF1 concentrations in both plasma and ULF in lean cows. The detrimental microenvironment created by IGF1 injection in lean cows and the lack of effect in obese cows resemble to a certain extent the situation observed in PCOS patients, where IGF1 bioavailability is increased in normal-weight women but reduced in obese women, suggesting that this bovine model could be useful for studying IGF1 involvement in PCOS.
0022-4251
41-52
Velazquez, Miguel A
9be900d6-3b01-4d61-98c2-251b76032e7d
Hadeler, Klaus-Gerd
cfbeb5e3-6d69-41ed-9f60-d7550ed3af3a
Herrmann, Doris
56620162-0024-409a-aeef-51541d3fb2f9
Kues, Wilfried A.
d80a0433-83b5-4587-a72e-bf9fe8d0a03e
Ulbrich, Susanne E.
9b75b12e-76da-40a4-baf6-b20561cccc68
Ulbrich, Susanne
0514a3a2-83dd-40a5-bd7f-3ad6e14249a3
Meyer, Heinrich H.D.
3727c498-bee8-40fa-a03a-c8f8ccf25fad
Rémy, Benoît
617310d9-1c11-4a65-814e-cb2ddf39d550
Beckers, Jean-François
dfe00b34-e989-4222-b973-7610cebaa2ad
Sauerwein, Helga
fc243dfe-0242-4f73-a850-ebb1a38469c1
Niemann, Heiner
47ee95f1-0530-4499-92a3-235a8ddffb5c
Velazquez, Miguel A
9be900d6-3b01-4d61-98c2-251b76032e7d
Hadeler, Klaus-Gerd
cfbeb5e3-6d69-41ed-9f60-d7550ed3af3a
Herrmann, Doris
56620162-0024-409a-aeef-51541d3fb2f9
Kues, Wilfried A.
d80a0433-83b5-4587-a72e-bf9fe8d0a03e
Ulbrich, Susanne E.
9b75b12e-76da-40a4-baf6-b20561cccc68
Ulbrich, Susanne
0514a3a2-83dd-40a5-bd7f-3ad6e14249a3
Meyer, Heinrich H.D.
3727c498-bee8-40fa-a03a-c8f8ccf25fad
Rémy, Benoît
617310d9-1c11-4a65-814e-cb2ddf39d550
Beckers, Jean-François
dfe00b34-e989-4222-b973-7610cebaa2ad
Sauerwein, Helga
fc243dfe-0242-4f73-a850-ebb1a38469c1
Niemann, Heiner
47ee95f1-0530-4499-92a3-235a8ddffb5c

Velazquez, Miguel A, Hadeler, Klaus-Gerd, Herrmann, Doris, Kues, Wilfried A., Ulbrich, Susanne E., Ulbrich, Susanne, Meyer, Heinrich H.D., Rémy, Benoît, Beckers, Jean-François, Sauerwein, Helga and Niemann, Heiner (2011) In vivo oocyte developmental competence is reduced in lean but not in obese superovulated dairy cows after intraovarian administration of IGF1. Reproduction, 142 (1), 41-52. (doi:10.1530/REP-10-0512). (PMID:21543511)

Record type: Article

Abstract

The present study investigated the role of IGF1 in lactating lean and non-lactating obese dairy cows by injecting 1??g IGF1 into the ovaries prior to superovulation. This amount of IGF1 has been linked with pregnancy loss in women with the polycystic ovary syndrome (PCOS) and was associated with impaired bovine oocyte competence in vitro. Transcript abundance and protein expression of selected genes involved in apoptosis, glucose metabolism, and the IGF system were analyzed. Plasma concentrations of IGF1 and leptin, and IGF1 in uterine luminal fluid (ULF), were also measured. IGF1 treatment decreased embryo viability in lean cows to the levels observed in obese cows. Obese cows were not affected by IGF1 treatment and showed elevated levels of IGF1 (in both plasma and ULF) and leptin. Blastocysts from lean cows treated with IGF1 showed a higher abundance of SLC2A1 and IGFBP3 transcripts. IGF1 treatment reduced protein expression of tumor protein 53 in blastocysts of lean cows, whereas the opposite was observed in obese cows. IGF1 in plasma and ULF was correlated only in the control groups. Blastocyst transcript abundance of IGF1 receptor and IGFBP3 correlated positively with IGF1 concentrations in both plasma and ULF in lean cows. The detrimental microenvironment created by IGF1 injection in lean cows and the lack of effect in obese cows resemble to a certain extent the situation observed in PCOS patients, where IGF1 bioavailability is increased in normal-weight women but reduced in obese women, suggesting that this bovine model could be useful for studying IGF1 involvement in PCOS.

Full text not available from this repository.

More information

Published date: July 2011
Organisations: Centre for Biological Sciences

Identifiers

Local EPrints ID: 369452
URI: http://eprints.soton.ac.uk/id/eprint/369452
ISSN: 0022-4251
PURE UUID: e1ea55b3-482c-43d7-accc-783e25a125c1

Catalogue record

Date deposited: 01 Oct 2014 16:23
Last modified: 15 Jul 2019 21:43

Export record

Altmetrics

Contributors

Author: Miguel A Velazquez
Author: Klaus-Gerd Hadeler
Author: Doris Herrmann
Author: Wilfried A. Kues
Author: Susanne E. Ulbrich
Author: Susanne Ulbrich
Author: Heinrich H.D. Meyer
Author: Benoît Rémy
Author: Jean-François Beckers
Author: Helga Sauerwein
Author: Heiner Niemann

University divisions

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×