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Efficiency of two timed artificial insemination protocols in Murrah buffaloes managed under a semi-intensive system in the tropics

Efficiency of two timed artificial insemination protocols in Murrah buffaloes managed under a semi-intensive system in the tropics
Efficiency of two timed artificial insemination protocols in Murrah buffaloes managed under a semi-intensive system in the tropics
The objective of the study was to determine the efficiency of ovsynch (OV) versus presynch-ovsynch (P-OV) protocol for synchronization of ovulation and timed artificial insemination (TAI) in female buffaloes. The OV group (n = 40) received gonadotrophin-releasing hormone (GnRH) on day 0 (random day of the estrous cycle), prostaglandin PGF?? on day 7 and a second GnRH administration on day 9 followed by a single artificial insemination (AI) 16-20 h later. The P-OV group (n = 40) received two PGF?? injections 14 days apart, with the second injection administered 14 days before starting the OV protocol. Progesterone (P(4)) was measured at the time of PGF?? administration (within the OV protocol) and AI. Neither ovulation rate ((24 h after TAI) OV 90%-36/40 vs. P-OV 85%-34/40) nor pregnancy rates ((day 60 after TAI) OV 35%-14/40 vs. P-OV 45%-18/40) differed between the two protocols. Pregnant buffaloes had lower concentrations of P(4) at AI compared with non-pregnant animals in the OV group (0.7 +/- 0.1 vs. 1.1 +/- 0.1 ng/ml); but in the P-OV group, differences did not reach statistical significance (0.8 +/- 0.1 vs. 1.0 +/- 0.1 ng/ml). This apparent trend reached statistical significance when the analysis was carried out in animals from both protocols (0.7 +/- 0.1 (pregnant) vs. 1.1 +/- 0.1 (non-pregnant) ng/ml). In conclusion, both protocols synchronize ovulation effectively with no significant differences in conception rates. High concentrations of P(4) at AI seem to be detrimental for the establishment of pregnancy in lactating buffalo cows.
ovsynch, presynch-ovsynch, lactating buffaloes, tropics
0049-4747
1149-1154
Oropeza, Armando José
0167fad4-0d68-4f4b-b69c-af332ddbc04a
Rojas, Angel F.
d22a3dcb-c0ee-4e1a-ab07-6b73ce4550cb
Velazquez, Miguel A.
0cb33ae4-eff7-445a-97a5-62a6daf71154
Muro, Juan D.
7d19185e-17bc-4b04-861f-11841a23bbfa
Márquez, Ysabel C.
ba5905f1-9985-4584-8750-699c11e48362
Vilanova, Lourdes T.
e8cdc87c-66f2-4d37-aa7d-1ea0dc9dc24c
Oropeza, Armando José
0167fad4-0d68-4f4b-b69c-af332ddbc04a
Rojas, Angel F.
d22a3dcb-c0ee-4e1a-ab07-6b73ce4550cb
Velazquez, Miguel A.
0cb33ae4-eff7-445a-97a5-62a6daf71154
Muro, Juan D.
7d19185e-17bc-4b04-861f-11841a23bbfa
Márquez, Ysabel C.
ba5905f1-9985-4584-8750-699c11e48362
Vilanova, Lourdes T.
e8cdc87c-66f2-4d37-aa7d-1ea0dc9dc24c

Oropeza, Armando José, Rojas, Angel F., Velazquez, Miguel A., Muro, Juan D., Márquez, Ysabel C. and Vilanova, Lourdes T. (2010) Efficiency of two timed artificial insemination protocols in Murrah buffaloes managed under a semi-intensive system in the tropics. Tropical Animal Health and Production, 42 (6), 1149-1154. (doi:10.1007/s11250-010-9539-9). (PMID:20358403)

Record type: Article

Abstract

The objective of the study was to determine the efficiency of ovsynch (OV) versus presynch-ovsynch (P-OV) protocol for synchronization of ovulation and timed artificial insemination (TAI) in female buffaloes. The OV group (n = 40) received gonadotrophin-releasing hormone (GnRH) on day 0 (random day of the estrous cycle), prostaglandin PGF?? on day 7 and a second GnRH administration on day 9 followed by a single artificial insemination (AI) 16-20 h later. The P-OV group (n = 40) received two PGF?? injections 14 days apart, with the second injection administered 14 days before starting the OV protocol. Progesterone (P(4)) was measured at the time of PGF?? administration (within the OV protocol) and AI. Neither ovulation rate ((24 h after TAI) OV 90%-36/40 vs. P-OV 85%-34/40) nor pregnancy rates ((day 60 after TAI) OV 35%-14/40 vs. P-OV 45%-18/40) differed between the two protocols. Pregnant buffaloes had lower concentrations of P(4) at AI compared with non-pregnant animals in the OV group (0.7 +/- 0.1 vs. 1.1 +/- 0.1 ng/ml); but in the P-OV group, differences did not reach statistical significance (0.8 +/- 0.1 vs. 1.0 +/- 0.1 ng/ml). This apparent trend reached statistical significance when the analysis was carried out in animals from both protocols (0.7 +/- 0.1 (pregnant) vs. 1.1 +/- 0.1 (non-pregnant) ng/ml). In conclusion, both protocols synchronize ovulation effectively with no significant differences in conception rates. High concentrations of P(4) at AI seem to be detrimental for the establishment of pregnancy in lactating buffalo cows.

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More information

Published date: August 2010
Keywords: ovsynch, presynch-ovsynch, lactating buffaloes, tropics
Organisations: Centre for Biological Sciences

Identifiers

Local EPrints ID: 369454
URI: http://eprints.soton.ac.uk/id/eprint/369454
ISSN: 0049-4747
PURE UUID: 96a94f3b-0330-4acc-8d64-e24ea1cdba00

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Date deposited: 01 Oct 2014 15:44
Last modified: 15 Jul 2019 21:43

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