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Developmental competence and mRNA expression of preimplantation in vitro-produced embryos from prepubertal and postpubertal cattle and their relationship with apoptosis after intraovarian administration of IGF-1.

Developmental competence and mRNA expression of preimplantation in vitro-produced embryos from prepubertal and postpubertal cattle and their relationship with apoptosis after intraovarian administration of IGF-1.
Developmental competence and mRNA expression of preimplantation in vitro-produced embryos from prepubertal and postpubertal cattle and their relationship with apoptosis after intraovarian administration of IGF-1.
Recombinant human Insulin-like growth factor-I (hIGF-1) was administered to one ovary of prepubertal and postpubertal cattle to determine its effects on (1) oocyte developmental competence, (2) the expression pattern of six developmentally important genes (GLUT3, GLUT8, AKT1, BCL-XL, BAD, and BAX), and (3) its relationship with apoptosis (female Holstein-Friesian). Oocytes were retrieved from 7- to 10-mo-old prepubertal dairy calves (preP), 11- to 18-mo-old postpubertal heifers (postP), and cows via ultrasound-guided follicular aspiration. Immature oocytes were matured in vitro then fertilized and cultured up to the blastocyst stage. Apoptosis was determined by terminal deoxynucleotidyl transferase nick-end labeling (TUNEL) in 8-d blastocysts. Similar low blastocyst yields were observed in the IGF-1-treated preP group (11.2+/-2.4%), the control preP group (10.4+/-3.0%), and in the IGF-1 postP group (10.9+/-2.3%). These were lower (P<or=0.01) compared with the control postP group (21.2+/-3.8%) and with cows (23+/-3.7%). The expression profile of the six genes was partly affected by age and IGF-1 treatment. Apoptosis was correlated with the age of the oocyte donors and was increased in blastocysts derived from prepubertal heifers. Results show that apoptosis is a critical feature of the acquisition of developmental competence of oocytes from prepubertal cattle and that IGF-1 did not beneficially affect oocyte developmental competence.
apoptosis, bovine embryos, IGF-1, in vitro, prepubertal donors
0093-691X
75-89
Zaraza, J.
fdaeb455-cbba-402c-b368-f3ae718aa799
Oropeza, A.
9f99d085-c8a9-430c-a8a5-24ea21b6d15c
Velazquez, M.A.
706e7de5-8cdb-4f2b-b47d-128166bdbea4
Korsawe, K.
e76cda1b-f268-4893-9697-290b30c282ee
Herrmann, D.
c8398914-03ee-4e83-a5ae-f6384f1ac103
Carnwath, J.W.
d86b6eec-2d37-4b67-ada1-a147f279fda4
Niemann, H.
1fcd6d64-751c-4bd6-bbf7-901368d89478
Zaraza, J.
fdaeb455-cbba-402c-b368-f3ae718aa799
Oropeza, A.
9f99d085-c8a9-430c-a8a5-24ea21b6d15c
Velazquez, M.A.
706e7de5-8cdb-4f2b-b47d-128166bdbea4
Korsawe, K.
e76cda1b-f268-4893-9697-290b30c282ee
Herrmann, D.
c8398914-03ee-4e83-a5ae-f6384f1ac103
Carnwath, J.W.
d86b6eec-2d37-4b67-ada1-a147f279fda4
Niemann, H.
1fcd6d64-751c-4bd6-bbf7-901368d89478

Zaraza, J., Oropeza, A., Velazquez, M.A., Korsawe, K., Herrmann, D., Carnwath, J.W. and Niemann, H. (2010) Developmental competence and mRNA expression of preimplantation in vitro-produced embryos from prepubertal and postpubertal cattle and their relationship with apoptosis after intraovarian administration of IGF-1. Theriogenology, 74 (1), 75-89. (doi:10.1016/j.theriogenology.2009.11.033). (PMID:20138354)

Record type: Article

Abstract

Recombinant human Insulin-like growth factor-I (hIGF-1) was administered to one ovary of prepubertal and postpubertal cattle to determine its effects on (1) oocyte developmental competence, (2) the expression pattern of six developmentally important genes (GLUT3, GLUT8, AKT1, BCL-XL, BAD, and BAX), and (3) its relationship with apoptosis (female Holstein-Friesian). Oocytes were retrieved from 7- to 10-mo-old prepubertal dairy calves (preP), 11- to 18-mo-old postpubertal heifers (postP), and cows via ultrasound-guided follicular aspiration. Immature oocytes were matured in vitro then fertilized and cultured up to the blastocyst stage. Apoptosis was determined by terminal deoxynucleotidyl transferase nick-end labeling (TUNEL) in 8-d blastocysts. Similar low blastocyst yields were observed in the IGF-1-treated preP group (11.2+/-2.4%), the control preP group (10.4+/-3.0%), and in the IGF-1 postP group (10.9+/-2.3%). These were lower (P<or=0.01) compared with the control postP group (21.2+/-3.8%) and with cows (23+/-3.7%). The expression profile of the six genes was partly affected by age and IGF-1 treatment. Apoptosis was correlated with the age of the oocyte donors and was increased in blastocysts derived from prepubertal heifers. Results show that apoptosis is a critical feature of the acquisition of developmental competence of oocytes from prepubertal cattle and that IGF-1 did not beneficially affect oocyte developmental competence.

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More information

Published date: 1 July 2010
Keywords: apoptosis, bovine embryos, IGF-1, in vitro, prepubertal donors
Organisations: Centre for Biological Sciences

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Local EPrints ID: 369455
URI: http://eprints.soton.ac.uk/id/eprint/369455
ISSN: 0093-691X
PURE UUID: 450d2520-eb6a-4039-9810-30cea0a1efc9

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Date deposited: 01 Oct 2014 15:39
Last modified: 15 Jul 2019 21:42

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