The University of Southampton
University of Southampton Institutional Repository

The usefulness of a single measurement of insulin-like growth factor-1 as a predictor of embryo yield and pregnancy rates in a bovine MOET program

The usefulness of a single measurement of insulin-like growth factor-1 as a predictor of embryo yield and pregnancy rates in a bovine MOET program
The usefulness of a single measurement of insulin-like growth factor-1 as a predictor of embryo yield and pregnancy rates in a bovine MOET program
The objective was to determine if a single measurement of plasma insulin-like growth factor-1 (IGF-1) could predict the number of viable embryos obtained from donors and the likelihood of pregnancy in recipients in multiple ovulation and embryo transfer (MOET) programs in cattle. The embryo yields from 101 embryo recoveries were examined in maiden Holstein heifers (n=75) and multiparous Holstein cows (lactating cows n=20, dry cows n=6). Donors were super stimulated with FSH and embryo recovery was done non-surgically 7 days after artificial insemination. Embryos were classified according to the IETS criteria. Pregnancy rates in 100 maiden Holstein heifer recipients were analysed. Recipients were on day 7+/-1 of the estrous cycle at transfer. Pregnancy diagnosis was carried out at day 30 (PD 30) and rechecked at day 60 (PD 60) after transfer. Blood samples from coccygeal vessels taken at the time of embryo recovery (donors) and transfer (recipients) were analysed for IGF-1, insulin, beta-hydroxybutyrate (beta-OHB), non-esterified fatty acids (NEFA), urea and cholesterol. There was a negative correlation between the number of viable embryos and insulin (r=-0.33, P=0.025) in donor heifers. In donor cows, the number of viable embryos was correlated with IGF-1 (r=0.43, P=0.028) and cholesterol (r=-0.43, P=0.027). In recipients, PD30 and PD 60 were not affected by any of the circulating parameters analysed. Insulin, IGF-1 and cholesterol only explained 8.9, 13.9 and 15.8% of the variation in the production of viable embryos, respectively. Several factors affect MOET programs and under the circumstances of the present study the usefulness of hormonal and metabolic profiles as predictors of the outcome of this biotechnology was limited.
IGF-1, superovulation, embryo transfer, cattle
0093-691X
1977-1994
Velazquez, M.A.
706e7de5-8cdb-4f2b-b47d-128166bdbea4
Newman, M.
18862192-d5f1-4765-913c-61e63e3e3d05
Christie, M.F.
ba37d129-ee12-471c-a413-0ac34eee3025
Cripps, P.J.
8d5e1c08-dddd-4eaa-b7b3-8ea931700db2
Crowe, M.A.
cee7380b-4f9b-4252-80f5-99fdbd616689
Smith, R.F.
2a029410-5955-4137-a1f1-1ed933b449cc
Dobson, H.
0ee400dd-c4ba-4cac-af1d-825bddaf47ca
Velazquez, M.A.
706e7de5-8cdb-4f2b-b47d-128166bdbea4
Newman, M.
18862192-d5f1-4765-913c-61e63e3e3d05
Christie, M.F.
ba37d129-ee12-471c-a413-0ac34eee3025
Cripps, P.J.
8d5e1c08-dddd-4eaa-b7b3-8ea931700db2
Crowe, M.A.
cee7380b-4f9b-4252-80f5-99fdbd616689
Smith, R.F.
2a029410-5955-4137-a1f1-1ed933b449cc
Dobson, H.
0ee400dd-c4ba-4cac-af1d-825bddaf47ca

Velazquez, M.A., Newman, M., Christie, M.F., Cripps, P.J., Crowe, M.A., Smith, R.F. and Dobson, H. (2005) The usefulness of a single measurement of insulin-like growth factor-1 as a predictor of embryo yield and pregnancy rates in a bovine MOET program. Theriogenology, 64 (9), 1977-1994. (doi:10.1016/j.theriogenology.2005.05.001). (PMID:15936811)

Record type: Article

Abstract

The objective was to determine if a single measurement of plasma insulin-like growth factor-1 (IGF-1) could predict the number of viable embryos obtained from donors and the likelihood of pregnancy in recipients in multiple ovulation and embryo transfer (MOET) programs in cattle. The embryo yields from 101 embryo recoveries were examined in maiden Holstein heifers (n=75) and multiparous Holstein cows (lactating cows n=20, dry cows n=6). Donors were super stimulated with FSH and embryo recovery was done non-surgically 7 days after artificial insemination. Embryos were classified according to the IETS criteria. Pregnancy rates in 100 maiden Holstein heifer recipients were analysed. Recipients were on day 7+/-1 of the estrous cycle at transfer. Pregnancy diagnosis was carried out at day 30 (PD 30) and rechecked at day 60 (PD 60) after transfer. Blood samples from coccygeal vessels taken at the time of embryo recovery (donors) and transfer (recipients) were analysed for IGF-1, insulin, beta-hydroxybutyrate (beta-OHB), non-esterified fatty acids (NEFA), urea and cholesterol. There was a negative correlation between the number of viable embryos and insulin (r=-0.33, P=0.025) in donor heifers. In donor cows, the number of viable embryos was correlated with IGF-1 (r=0.43, P=0.028) and cholesterol (r=-0.43, P=0.027). In recipients, PD30 and PD 60 were not affected by any of the circulating parameters analysed. Insulin, IGF-1 and cholesterol only explained 8.9, 13.9 and 15.8% of the variation in the production of viable embryos, respectively. Several factors affect MOET programs and under the circumstances of the present study the usefulness of hormonal and metabolic profiles as predictors of the outcome of this biotechnology was limited.

Full text not available from this repository.

More information

e-pub ahead of print date: 4 June 2005
Published date: December 2005
Keywords: IGF-1, superovulation, embryo transfer, cattle
Organisations: Centre for Biological Sciences

Identifiers

Local EPrints ID: 369460
URI: http://eprints.soton.ac.uk/id/eprint/369460
ISSN: 0093-691X
PURE UUID: dc53e823-74cc-4795-9a81-708ad7175737

Catalogue record

Date deposited: 15 Oct 2014 14:17
Last modified: 15 Jul 2019 21:42

Export record

Altmetrics

Contributors

Author: M.A. Velazquez
Author: M. Newman
Author: M.F. Christie
Author: P.J. Cripps
Author: M.A. Crowe
Author: R.F. Smith
Author: H. Dobson

University divisions

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×