Alternative mRNA splicing creates transcripts encoding soluble proteins from most LILR genes
Alternative mRNA splicing creates transcripts encoding soluble proteins from most LILR genes
Leucocyte Ig-like receptors (LILR) are a family of innate immune receptors expressed on myeloid and lymphoid cells that influence adaptive immune responses. We identified a common mechanism of alternative mRNA splicing, which generates transcripts that encode soluble protein isoforms of the majority of human LILR. These alternative splice variants lack transmembrane and cytoplasmic encoding regions, due to the transcription of a cryptic stop codon present in an intron 5' of the transmembrane encoding exon. The alternative LILR transcripts were detected in cell types that express their membrane-associated isoforms. Expression of the alternative LILRB1 transcript in transfected cells resulted in the release of a soluble approximately 65 Kd LILRB1 protein into culture supernatants. Soluble LILRB1 protein was also detected in the culture supernatants of monocyte-derived DC. In vitro assays suggested that soluble LILRB1 could block the interaction between membrane-associated LILRB1 and HLA-class I. Soluble LILRB1 may act as a dominant negative regulator of HLA-class I-mediated LILRB1 inhibition. Soluble isoforms of the other LILR may function in a comparable way.
alternative splicing, leucocyte Ig-like receptor, Ig-like transcript, secreted, soluble
3195-206
Jones, Des C.
f82edca9-6a9a-43c6-9378-7d435c833bc5
Roghanian, Ali
e2b032c2-60a0-4522-a3d8-56a768792f36
Brown, Damien P.
e92bfa7c-6283-4f9a-a96d-a29983aa0dcd
Chang, Chiwen
6282af1a-1b22-404a-83d2-e7a24fc29c69
Allen, Rachel L.
c8a8bf59-714c-4d05-8dd1-63b349e3f3ce
Trowsdale, John
5bbacc4a-45f4-4376-8357-a7c79fc4fe12
Young, Neil T.
420c5bc3-494e-4840-9af2-dc1b081291b1
November 2009
Jones, Des C.
f82edca9-6a9a-43c6-9378-7d435c833bc5
Roghanian, Ali
e2b032c2-60a0-4522-a3d8-56a768792f36
Brown, Damien P.
e92bfa7c-6283-4f9a-a96d-a29983aa0dcd
Chang, Chiwen
6282af1a-1b22-404a-83d2-e7a24fc29c69
Allen, Rachel L.
c8a8bf59-714c-4d05-8dd1-63b349e3f3ce
Trowsdale, John
5bbacc4a-45f4-4376-8357-a7c79fc4fe12
Young, Neil T.
420c5bc3-494e-4840-9af2-dc1b081291b1
Jones, Des C., Roghanian, Ali, Brown, Damien P., Chang, Chiwen, Allen, Rachel L., Trowsdale, John and Young, Neil T.
(2009)
Alternative mRNA splicing creates transcripts encoding soluble proteins from most LILR genes.
European Journal of Immunology, 39 (11), .
(doi:10.1002/eji.200839080).
(PMID:19658091)
Abstract
Leucocyte Ig-like receptors (LILR) are a family of innate immune receptors expressed on myeloid and lymphoid cells that influence adaptive immune responses. We identified a common mechanism of alternative mRNA splicing, which generates transcripts that encode soluble protein isoforms of the majority of human LILR. These alternative splice variants lack transmembrane and cytoplasmic encoding regions, due to the transcription of a cryptic stop codon present in an intron 5' of the transmembrane encoding exon. The alternative LILR transcripts were detected in cell types that express their membrane-associated isoforms. Expression of the alternative LILRB1 transcript in transfected cells resulted in the release of a soluble approximately 65 Kd LILRB1 protein into culture supernatants. Soluble LILRB1 protein was also detected in the culture supernatants of monocyte-derived DC. In vitro assays suggested that soluble LILRB1 could block the interaction between membrane-associated LILRB1 and HLA-class I. Soluble LILRB1 may act as a dominant negative regulator of HLA-class I-mediated LILRB1 inhibition. Soluble isoforms of the other LILR may function in a comparable way.
This record has no associated files available for download.
More information
e-pub ahead of print date: 5 August 2009
Published date: November 2009
Keywords:
alternative splicing, leucocyte Ig-like receptor, Ig-like transcript, secreted, soluble
Organisations:
Cancer Sciences
Identifiers
Local EPrints ID: 369610
URI: http://eprints.soton.ac.uk/id/eprint/369610
ISSN: 0014-2980
PURE UUID: 8fd5a934-3e2e-4c6a-9091-9bfcda6fdbf7
Catalogue record
Date deposited: 02 Oct 2014 10:36
Last modified: 15 Mar 2024 03:34
Export record
Altmetrics
Contributors
Author:
Des C. Jones
Author:
Damien P. Brown
Author:
Chiwen Chang
Author:
Rachel L. Allen
Author:
John Trowsdale
Author:
Neil T. Young
Download statistics
Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.
View more statistics