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EPR based distance measurement in Cu-porphyrin–DNA

EPR based distance measurement in Cu-porphyrin–DNA
EPR based distance measurement in Cu-porphyrin–DNA
EPR spectroscopy was used to investigate both single and double stranded DNA modified with a variable number of copper(II) porphyrins. The spectra of the porphyrin–DNA complexes resemble those of the Cu(II) porphyrin building blocks, but with appreciable differences in the values for the g? and A? parameters. In addition, a significant half-field signal is observed, which is interpreted as resulting from copper–copper interactions in both the double strand (dsDNA) and the single strand DNA (ssDNA). Analysis of the EPR spectra gives evidence for cluster formation of three or more DNA strands. From the intensity ratio of the half-field and main transition, the average Cu–Cu distance is estimated to be 6.5–8.9 Å. The association of copper centres is consistent with hydrophobic porphyrin stacking, both intra- and intermolecularly, as has previously been observed with other DNA complexes using UV-vis and CD spectroscopy.
1144-0546
1-6
Nguyen, ThaoNguyen
dfe06677-7b72-4616-a409-4b8ada09c5f4
Håkansson, Pär
814e08b5-ae95-408f-a669-bec9c0ef4235
Edge, Ruth
4020c23f-6101-48da-ab31-c87b8b777605
Collison, David
414a567a-1af9-4780-a6de-726f58a42349
Goodman, Bernard A.
b978cf02-04ca-469c-b00f-c85939d5c185
Burns, Jonathan R.
648a50f5-bcca-4989-8de3-60cc582b7ed9
Stulz, Eugen
9a6c04cf-32ca-442b-9281-bbf3d23c622d
Nguyen, ThaoNguyen
dfe06677-7b72-4616-a409-4b8ada09c5f4
Håkansson, Pär
814e08b5-ae95-408f-a669-bec9c0ef4235
Edge, Ruth
4020c23f-6101-48da-ab31-c87b8b777605
Collison, David
414a567a-1af9-4780-a6de-726f58a42349
Goodman, Bernard A.
b978cf02-04ca-469c-b00f-c85939d5c185
Burns, Jonathan R.
648a50f5-bcca-4989-8de3-60cc582b7ed9
Stulz, Eugen
9a6c04cf-32ca-442b-9281-bbf3d23c622d

Nguyen, ThaoNguyen, Håkansson, Pär, Edge, Ruth, Collison, David, Goodman, Bernard A., Burns, Jonathan R. and Stulz, Eugen (2014) EPR based distance measurement in Cu-porphyrin–DNA. New Journal of Chemistry, 1-6. (doi:10.1039/C4NJ00673A).

Record type: Article

Abstract

EPR spectroscopy was used to investigate both single and double stranded DNA modified with a variable number of copper(II) porphyrins. The spectra of the porphyrin–DNA complexes resemble those of the Cu(II) porphyrin building blocks, but with appreciable differences in the values for the g? and A? parameters. In addition, a significant half-field signal is observed, which is interpreted as resulting from copper–copper interactions in both the double strand (dsDNA) and the single strand DNA (ssDNA). Analysis of the EPR spectra gives evidence for cluster formation of three or more DNA strands. From the intensity ratio of the half-field and main transition, the average Cu–Cu distance is estimated to be 6.5–8.9 Å. The association of copper centres is consistent with hydrophobic porphyrin stacking, both intra- and intermolecularly, as has previously been observed with other DNA complexes using UV-vis and CD spectroscopy.

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e-pub ahead of print date: 19 August 2014
Organisations: Chemistry

Identifiers

Local EPrints ID: 369855
URI: http://eprints.soton.ac.uk/id/eprint/369855
ISSN: 1144-0546
PURE UUID: 67f443cd-629c-4e63-a498-2b809c4ee4c7
ORCID for Eugen Stulz: ORCID iD orcid.org/0000-0002-5302-2276

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Date deposited: 08 Oct 2014 14:53
Last modified: 20 Jul 2019 00:54

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