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The application of optical coherence tomography to image subsurface tissue structure of Antarctic krill Euphausia superba

The application of optical coherence tomography to image subsurface tissue structure of Antarctic krill Euphausia superba
The application of optical coherence tomography to image subsurface tissue structure of Antarctic krill Euphausia superba
Many small open ocean animals, such as Antarctic krill, are an important part of marine ecosystems. To discover what will happen to animals such as krill in a changing ocean, experiments are run in aquaria where conditions can be controlled to simulate water characteristics predicted to occur in the future. The response of individual animals to changing water conditions can be hard to observe, and with current observation techniques it is very difficult to follow the progress of an individual animal through its life. Optical coherence tomography (OCT) is an optical imaging technique that allows images at high resolution to be obtained from depths up to a few millimeters inside biological specimens. It is compatible with in vivo imaging and can be used repeatedly on the same specimens. In this work, we show how OCT may be applied to post mortem krill samples and how important physiological data such as shell thickness and estimates of organ volume can be obtained. Using OCT we find an average value for the thickness of krill exoskeleton to be (30±4) µm along a 1 cm length of the animal body. We also show that the technique may be used to provide detailed imagery of the internal structure of a pleopod joint and provide an estimate for the heart volume of (0.73±0.03) mm3.
1932-6203
1-7
Bellini, Nicola
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Cox, Martin J.
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Harper, Danielle J.
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Stott, Sebastian R.
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Ashok, Praveen C.
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Dholakia, Kishan
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Kawaguchi, So
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King, Robert
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Horton, Tammy
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Brown, Christian T.A.
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Bellini, Nicola
9ac9664d-804e-492f-8410-619411ef9d0d
Cox, Martin J.
dae8a5f5-f2c8-4ebf-b8dc-b26913c67f87
Harper, Danielle J.
4690587d-d144-4cfc-b466-8777863bd670
Stott, Sebastian R.
fa4d9247-b427-48e6-b5b1-332b46574cf4
Ashok, Praveen C.
7981e565-555a-447e-9a87-7509ce253a0d
Dholakia, Kishan
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Kawaguchi, So
802fedb6-f8e4-4bf9-aeb1-c04da22e3d6b
King, Robert
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Horton, Tammy
c4b41665-f0bc-4f0f-a7af-b2b9afc02e34
Brown, Christian T.A.
b51e6c14-b365-484a-a23f-82858e0a9eb6

Bellini, Nicola, Cox, Martin J., Harper, Danielle J., Stott, Sebastian R., Ashok, Praveen C., Dholakia, Kishan, Kawaguchi, So, King, Robert, Horton, Tammy and Brown, Christian T.A. (2014) The application of optical coherence tomography to image subsurface tissue structure of Antarctic krill Euphausia superba. PLoS ONE, 9 (10), 1-7. (doi:10.1371/journal.pone.0110367).

Record type: Article

Abstract

Many small open ocean animals, such as Antarctic krill, are an important part of marine ecosystems. To discover what will happen to animals such as krill in a changing ocean, experiments are run in aquaria where conditions can be controlled to simulate water characteristics predicted to occur in the future. The response of individual animals to changing water conditions can be hard to observe, and with current observation techniques it is very difficult to follow the progress of an individual animal through its life. Optical coherence tomography (OCT) is an optical imaging technique that allows images at high resolution to be obtained from depths up to a few millimeters inside biological specimens. It is compatible with in vivo imaging and can be used repeatedly on the same specimens. In this work, we show how OCT may be applied to post mortem krill samples and how important physiological data such as shell thickness and estimates of organ volume can be obtained. Using OCT we find an average value for the thickness of krill exoskeleton to be (30±4) µm along a 1 cm length of the animal body. We also show that the technique may be used to provide detailed imagery of the internal structure of a pleopod joint and provide an estimate for the heart volume of (0.73±0.03) mm3.

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Published date: 13 October 2014
Organisations: Marine Biogeochemistry

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Local EPrints ID: 370108
URI: http://eprints.soton.ac.uk/id/eprint/370108
ISSN: 1932-6203
PURE UUID: 350ef8b8-52a4-4bd3-a262-5cc4b4906b90

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Date deposited: 15 Oct 2014 09:06
Last modified: 14 Mar 2024 18:12

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Contributors

Author: Nicola Bellini
Author: Martin J. Cox
Author: Danielle J. Harper
Author: Sebastian R. Stott
Author: Praveen C. Ashok
Author: Kishan Dholakia
Author: So Kawaguchi
Author: Robert King
Author: Tammy Horton
Author: Christian T.A. Brown

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