Interactions of skin with gold nanoparticles of different surface charge, shape, and functionality
Interactions of skin with gold nanoparticles of different surface charge, shape, and functionality
The interactions between skin and colloidal gold nanoparticles of different physicochemical characteristics are investigated. By systematically varying the charge, shape, and functionality of gold nanoparticles, the nanoparticle penetration through the different skin layers is assessed. The penetration is evaluated both qualitatively and quantitatively using a variety of complementary techniques. Inductively coupled plasma optical emission spectrometry (ICP-OES) is used to quantify the total number of particles which penetrate the skin structure. Transmission electron microscopy (TEM) and two photon photoluminescence microscopy (TPPL) on skin cross sections provide a direct visualization of nanoparticle migration within the different skin substructures. These studies reveal that gold nanoparticles functionalized with cell penetrating peptides (CPPs) TAT and R7 are found in the skin in larger quantities than polyethylene glycol-functionalized nanoparticles, and are able to enter deep into the skin structure. The systematic studies presented in this work may be of strong interest for developments in transdermal administration of drugs and therapy
713-721
Fernandes, Rute
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Smyth, Neil R.
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Muskens, Otto L.
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Nitti, Simone
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Heuer-Jungemann, Amelie
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Ardern-Jones, Michael R.
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Kanaras, Antonios G.
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11 February 2015
Fernandes, Rute
848317d8-1eb0-48ad-aefc-012ab19d9f52
Smyth, Neil R.
0eba2a40-3b43-4d40-bb64-621bd7e9d505
Muskens, Otto L.
2284101a-f9ef-4d79-8951-a6cda5bfc7f9
Nitti, Simone
aee69803-b6d1-4bf9-9f7f-84955333cd8d
Heuer-Jungemann, Amelie
19d5a272-0f65-4679-b62b-263fa1806230
Ardern-Jones, Michael R.
7ac43c24-94ab-4d19-ba69-afaa546bec90
Kanaras, Antonios G.
667ecfdc-7647-4bd8-be03-a47bf32504c7
Fernandes, Rute, Smyth, Neil R., Muskens, Otto L., Nitti, Simone, Heuer-Jungemann, Amelie, Ardern-Jones, Michael R. and Kanaras, Antonios G.
(2015)
Interactions of skin with gold nanoparticles of different surface charge, shape, and functionality.
Small, 11 (6), .
(doi:10.1002/smll.201401913).
(PMID:25288531)
Abstract
The interactions between skin and colloidal gold nanoparticles of different physicochemical characteristics are investigated. By systematically varying the charge, shape, and functionality of gold nanoparticles, the nanoparticle penetration through the different skin layers is assessed. The penetration is evaluated both qualitatively and quantitatively using a variety of complementary techniques. Inductively coupled plasma optical emission spectrometry (ICP-OES) is used to quantify the total number of particles which penetrate the skin structure. Transmission electron microscopy (TEM) and two photon photoluminescence microscopy (TPPL) on skin cross sections provide a direct visualization of nanoparticle migration within the different skin substructures. These studies reveal that gold nanoparticles functionalized with cell penetrating peptides (CPPs) TAT and R7 are found in the skin in larger quantities than polyethylene glycol-functionalized nanoparticles, and are able to enter deep into the skin structure. The systematic studies presented in this work may be of strong interest for developments in transdermal administration of drugs and therapy
Text
Revised_Small _Kanaras manuscript.doc
- Accepted Manuscript
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e-pub ahead of print date: 7 October 2014
Published date: 11 February 2015
Organisations:
Chemistry, Physics & Astronomy, Centre for Biological Sciences
Identifiers
Local EPrints ID: 370231
URI: http://eprints.soton.ac.uk/id/eprint/370231
ISSN: 1613-6810
PURE UUID: 20b220cd-869d-48c9-88fe-04f67de77817
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Date deposited: 24 Oct 2014 15:24
Last modified: 15 Mar 2024 03:34
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Author:
Rute Fernandes
Author:
Simone Nitti
Author:
Amelie Heuer-Jungemann
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