The University of Southampton
University of Southampton Institutional Repository

Microbial diversity and biogenic methane potential of a thermogenic-gas coal mine

Microbial diversity and biogenic methane potential of a thermogenic-gas coal mine
Microbial diversity and biogenic methane potential of a thermogenic-gas coal mine
The microbial communities and biogenic methane potential of a gas coal mine were investigated by cultivation-independent and cultivation-dependent approaches. Stable carbon isotopic analysis indicated that in situ methane in the coal mine was dominantly of a thermogenic origin. However, a high level of diversity of bacteria and methanogens that were present in the coal mine was revealed by 454 pyrosequencing, and included various fermentative bacteria in the phyla of Actinobacteria, Bacteroidetes, Firmicutes, and Proteobacteria, and acetotrophic, hydrogenotrophic, and methylotrophic methanogens. Methane was produced in enrichments of mine water samples supplemented with acetate under laboratory conditions. The microbial flora obtained from the enrichments could stimulate methane formation from coal samples. 16S rRNA gene clone library analysis indicated that the microbial community from coal cultivation samples supplemented with the enriched microbial consortium was dominated by the anaerobic fermentative Clostridiales and facultative acetoclastic Methanosarcina. This study suggests that the biogenic methane potential in the thermogenic-gas coal mine could be stimulated by the indigenous microorganisms.
biogenic methane, acetotrophic methanogen, 454 pyrosequencing, real-time PCR
0166-5162
96-107
Wei, Min
28a811b4-8861-44fd-84ae-7b9e38dd6086
Yu, Zhisheng
1a0ec40b-125b-4917-a77f-c8c4c3e00dda
Jiang, Zheng
bcf19e78-f5c3-48e6-802b-fe77bd12deab
Zhang, Hongxun
7005c6e2-91b8-43b7-a5d2-2378d3a08445
Wei, Min
28a811b4-8861-44fd-84ae-7b9e38dd6086
Yu, Zhisheng
1a0ec40b-125b-4917-a77f-c8c4c3e00dda
Jiang, Zheng
bcf19e78-f5c3-48e6-802b-fe77bd12deab
Zhang, Hongxun
7005c6e2-91b8-43b7-a5d2-2378d3a08445

Wei, Min, Yu, Zhisheng, Jiang, Zheng and Zhang, Hongxun (2014) Microbial diversity and biogenic methane potential of a thermogenic-gas coal mine. International Journal of Coal Geology, 134-135, 96-107. (doi:10.1016/j.coal.2014.09.008).

Record type: Article

Abstract

The microbial communities and biogenic methane potential of a gas coal mine were investigated by cultivation-independent and cultivation-dependent approaches. Stable carbon isotopic analysis indicated that in situ methane in the coal mine was dominantly of a thermogenic origin. However, a high level of diversity of bacteria and methanogens that were present in the coal mine was revealed by 454 pyrosequencing, and included various fermentative bacteria in the phyla of Actinobacteria, Bacteroidetes, Firmicutes, and Proteobacteria, and acetotrophic, hydrogenotrophic, and methylotrophic methanogens. Methane was produced in enrichments of mine water samples supplemented with acetate under laboratory conditions. The microbial flora obtained from the enrichments could stimulate methane formation from coal samples. 16S rRNA gene clone library analysis indicated that the microbial community from coal cultivation samples supplemented with the enriched microbial consortium was dominated by the anaerobic fermentative Clostridiales and facultative acetoclastic Methanosarcina. This study suggests that the biogenic methane potential in the thermogenic-gas coal mine could be stimulated by the indigenous microorganisms.

Text
__soton.ac.uk_ude_personalfiles_users_zj1m12_mydesktop_1-s2.0-S0166516214001943-main.pdf - Accepted Manuscript
Download (1MB)

More information

e-pub ahead of print date: 21 September 2014
Published date: 15 November 2014
Keywords: biogenic methane, acetotrophic methanogen, 454 pyrosequencing, real-time PCR
Organisations: Faculty of Engineering and the Environment

Identifiers

Local EPrints ID: 370271
URI: http://eprints.soton.ac.uk/id/eprint/370271
ISSN: 0166-5162
PURE UUID: 234014a5-a2d9-494e-b2a9-dd99f449d507
ORCID for Zheng Jiang: ORCID iD orcid.org/0000-0002-7972-6175

Catalogue record

Date deposited: 28 Oct 2014 11:37
Last modified: 26 Nov 2019 01:35

Export record

Altmetrics

Contributors

Author: Min Wei
Author: Zhisheng Yu
Author: Zheng Jiang ORCID iD
Author: Hongxun Zhang

University divisions

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×