Microbial diversity and biogenic methane potential of a thermogenic-gas coal mine
Microbial diversity and biogenic methane potential of a thermogenic-gas coal mine
The microbial communities and biogenic methane potential of a gas coal mine were investigated by cultivation-independent and cultivation-dependent approaches. Stable carbon isotopic analysis indicated that in situ methane in the coal mine was dominantly of a thermogenic origin. However, a high level of diversity of bacteria and methanogens that were present in the coal mine was revealed by 454 pyrosequencing, and included various fermentative bacteria in the phyla of Actinobacteria, Bacteroidetes, Firmicutes, and Proteobacteria, and acetotrophic, hydrogenotrophic, and methylotrophic methanogens. Methane was produced in enrichments of mine water samples supplemented with acetate under laboratory conditions. The microbial flora obtained from the enrichments could stimulate methane formation from coal samples. 16S rRNA gene clone library analysis indicated that the microbial community from coal cultivation samples supplemented with the enriched microbial consortium was dominated by the anaerobic fermentative Clostridiales and facultative acetoclastic Methanosarcina. This study suggests that the biogenic methane potential in the thermogenic-gas coal mine could be stimulated by the indigenous microorganisms.
biogenic methane, acetotrophic methanogen, 454 pyrosequencing, real-time PCR
96-107
Wei, Min
28a811b4-8861-44fd-84ae-7b9e38dd6086
Yu, Zhisheng
1a0ec40b-125b-4917-a77f-c8c4c3e00dda
Jiang, Zheng
bcf19e78-f5c3-48e6-802b-fe77bd12deab
Zhang, Hongxun
7005c6e2-91b8-43b7-a5d2-2378d3a08445
15 November 2014
Wei, Min
28a811b4-8861-44fd-84ae-7b9e38dd6086
Yu, Zhisheng
1a0ec40b-125b-4917-a77f-c8c4c3e00dda
Jiang, Zheng
bcf19e78-f5c3-48e6-802b-fe77bd12deab
Zhang, Hongxun
7005c6e2-91b8-43b7-a5d2-2378d3a08445
Wei, Min, Yu, Zhisheng, Jiang, Zheng and Zhang, Hongxun
(2014)
Microbial diversity and biogenic methane potential of a thermogenic-gas coal mine.
International Journal of Coal Geology, 134-135, .
(doi:10.1016/j.coal.2014.09.008).
Abstract
The microbial communities and biogenic methane potential of a gas coal mine were investigated by cultivation-independent and cultivation-dependent approaches. Stable carbon isotopic analysis indicated that in situ methane in the coal mine was dominantly of a thermogenic origin. However, a high level of diversity of bacteria and methanogens that were present in the coal mine was revealed by 454 pyrosequencing, and included various fermentative bacteria in the phyla of Actinobacteria, Bacteroidetes, Firmicutes, and Proteobacteria, and acetotrophic, hydrogenotrophic, and methylotrophic methanogens. Methane was produced in enrichments of mine water samples supplemented with acetate under laboratory conditions. The microbial flora obtained from the enrichments could stimulate methane formation from coal samples. 16S rRNA gene clone library analysis indicated that the microbial community from coal cultivation samples supplemented with the enriched microbial consortium was dominated by the anaerobic fermentative Clostridiales and facultative acetoclastic Methanosarcina. This study suggests that the biogenic methane potential in the thermogenic-gas coal mine could be stimulated by the indigenous microorganisms.
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More information
e-pub ahead of print date: 21 September 2014
Published date: 15 November 2014
Keywords:
biogenic methane, acetotrophic methanogen, 454 pyrosequencing, real-time PCR
Organisations:
Faculty of Engineering and the Environment
Identifiers
Local EPrints ID: 370271
URI: http://eprints.soton.ac.uk/id/eprint/370271
ISSN: 0166-5162
PURE UUID: 234014a5-a2d9-494e-b2a9-dd99f449d507
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Date deposited: 28 Oct 2014 11:37
Last modified: 15 Mar 2024 03:47
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Contributors
Author:
Min Wei
Author:
Zhisheng Yu
Author:
Hongxun Zhang
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