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Enhancer RNA facilitates NELF release from immediate early genes

Enhancer RNA facilitates NELF release from immediate early genes
Enhancer RNA facilitates NELF release from immediate early genes
Enhancer RNAs (eRNAs) are a class of long noncoding RNAs (lncRNA) expressed from active enhancers, whose function and action mechanism are yet to be firmly established. Here we show that eRNAs facilitate the transition of paused RNA polymerase II (RNAPII) into productive elongation by acting as a decoy for the negative elongation factor (NELF) complex upon induction of immediate early genes (IEGs) in neurons. eRNAs are synthesized prior to the culmination of target gene transcription and interact with the NELF complex. Knockdown of eRNAs expressed at neuronal enhancers impairs transient release of NELF from the specific target promoters during transcriptional activation, coinciding with a decrease in target mRNA induction. The enhancer-promoter interaction was unaffected by eRNA knockdown. Instead, chromatin looping might enable eRNAs to act locally at a specific promoter. Our findings highlight the spatiotemporally regulated action mechanism of eRNAs during early transcriptional elongation.
1097-2765
29-42
Schaukowitch, Katie
a5480138-c207-4c43-bcd0-a24d7e2359ea
Joo, Jae-Yeol
94f88ddc-9ac2-4364-b8dd-a0e8ee8795cd
Liu, Xihui
cf748cd2-1a7b-4b0b-8d6d-49e4f57926cf
Watts, Jonathan K.
c4de85ee-aaa3-4e7d-99b3-147a4de4f01c
Martinez, Carlos
26b5384e-3a0a-49da-b8d2-919e755f876b
Kim, Tae-Kyung
3c226a07-f9c0-4e2d-9f8f-83532c6176ef
Schaukowitch, Katie
a5480138-c207-4c43-bcd0-a24d7e2359ea
Joo, Jae-Yeol
94f88ddc-9ac2-4364-b8dd-a0e8ee8795cd
Liu, Xihui
cf748cd2-1a7b-4b0b-8d6d-49e4f57926cf
Watts, Jonathan K.
c4de85ee-aaa3-4e7d-99b3-147a4de4f01c
Martinez, Carlos
26b5384e-3a0a-49da-b8d2-919e755f876b
Kim, Tae-Kyung
3c226a07-f9c0-4e2d-9f8f-83532c6176ef

Schaukowitch, Katie, Joo, Jae-Yeol, Liu, Xihui, Watts, Jonathan K., Martinez, Carlos and Kim, Tae-Kyung (2014) Enhancer RNA facilitates NELF release from immediate early genes. Molecular Cell, 56 (1), 29-42. (doi:10.1016/j.molcel.2014.08.023).

Record type: Article

Abstract

Enhancer RNAs (eRNAs) are a class of long noncoding RNAs (lncRNA) expressed from active enhancers, whose function and action mechanism are yet to be firmly established. Here we show that eRNAs facilitate the transition of paused RNA polymerase II (RNAPII) into productive elongation by acting as a decoy for the negative elongation factor (NELF) complex upon induction of immediate early genes (IEGs) in neurons. eRNAs are synthesized prior to the culmination of target gene transcription and interact with the NELF complex. Knockdown of eRNAs expressed at neuronal enhancers impairs transient release of NELF from the specific target promoters during transcriptional activation, coinciding with a decrease in target mRNA induction. The enhancer-promoter interaction was unaffected by eRNA knockdown. Instead, chromatin looping might enable eRNAs to act locally at a specific promoter. Our findings highlight the spatiotemporally regulated action mechanism of eRNAs during early transcriptional elongation.

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More information

Accepted/In Press date: 19 August 2014
e-pub ahead of print date: 25 September 2014
Published date: 2 October 2014

Identifiers

Local EPrints ID: 371667
URI: http://eprints.soton.ac.uk/id/eprint/371667
ISSN: 1097-2765
PURE UUID: c2f8a494-6a83-4b36-95b8-30f44796057f

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Date deposited: 12 Nov 2014 11:40
Last modified: 14 Mar 2024 18:24

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Contributors

Author: Katie Schaukowitch
Author: Jae-Yeol Joo
Author: Xihui Liu
Author: Jonathan K. Watts
Author: Carlos Martinez
Author: Tae-Kyung Kim

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