Nucleoside-tailored molecularly imprinted polymeric nanoparticles (MIP NPs)
Nucleoside-tailored molecularly imprinted polymeric nanoparticles (MIP NPs)
MIP NPs represent one of the current most suitable alternatives to antibodies for molecular recognition and diagnostic applications. Here we present the synthesis of MIP NPs imprinted for 2'-deoxyadenosine (dA) prepared using for the first time a modified polymerizable 2'-deoxyuridine complementary residue. We demonstrate that the introduction of this modified monomer results in an increase of the affinity of the produced MIP NPs, without altering their physical properties such as size, shape, or dispersibility. The imprinted NPs have shown the ability to specifically recognize dA, whereas no imprinting effect was observed for 2'-deoxyguanosine (dG) or deoxycytidine (dC). The results suggest that such monomers (and their phosphoramidites) could be used in the synthesis of oligomer or longer DNA sequences for potentially producing hybrid MIP–DNA materials with improved recognition performance.
6322-6330
Poma, Alessandro
9c796d9f-b5c2-417c-914f-6aecc51acd66
Brahmbhatt, Heli
9a1bc7b7-46f2-4d60-ad99-a80392bb4a70
Watts, Jonathan K.
c4de85ee-aaa3-4e7d-99b3-147a4de4f01c
Turner, Nicholas W.
3a137029-97b1-4142-be13-8444cfb04c9e
12 September 2014
Poma, Alessandro
9c796d9f-b5c2-417c-914f-6aecc51acd66
Brahmbhatt, Heli
9a1bc7b7-46f2-4d60-ad99-a80392bb4a70
Watts, Jonathan K.
c4de85ee-aaa3-4e7d-99b3-147a4de4f01c
Turner, Nicholas W.
3a137029-97b1-4142-be13-8444cfb04c9e
Poma, Alessandro, Brahmbhatt, Heli, Watts, Jonathan K. and Turner, Nicholas W.
(2014)
Nucleoside-tailored molecularly imprinted polymeric nanoparticles (MIP NPs).
Macromolecules, 47 (18), .
(doi:10.1021/ma501530c).
Abstract
MIP NPs represent one of the current most suitable alternatives to antibodies for molecular recognition and diagnostic applications. Here we present the synthesis of MIP NPs imprinted for 2'-deoxyadenosine (dA) prepared using for the first time a modified polymerizable 2'-deoxyuridine complementary residue. We demonstrate that the introduction of this modified monomer results in an increase of the affinity of the produced MIP NPs, without altering their physical properties such as size, shape, or dispersibility. The imprinted NPs have shown the ability to specifically recognize dA, whereas no imprinting effect was observed for 2'-deoxyguanosine (dG) or deoxycytidine (dC). The results suggest that such monomers (and their phosphoramidites) could be used in the synthesis of oligomer or longer DNA sequences for potentially producing hybrid MIP–DNA materials with improved recognition performance.
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e-pub ahead of print date: 12 September 2014
Published date: 12 September 2014
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Local EPrints ID: 371669
URI: http://eprints.soton.ac.uk/id/eprint/371669
PURE UUID: c325baf5-e99c-4928-a345-21b96a8c200d
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Date deposited: 12 Nov 2014 11:42
Last modified: 14 Mar 2024 18:24
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Author:
Alessandro Poma
Author:
Heli Brahmbhatt
Author:
Jonathan K. Watts
Author:
Nicholas W. Turner
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