Microstructural characterization of CPPD and hydroxyapatite crystal depositions on human menisci
Microstructural characterization of CPPD and hydroxyapatite crystal depositions on human menisci
Meniscus is a fibrocartilaginous tissue composed mainly of water and a dense elaborate collagen network with a predominantly circumferential alignment. Crystal formation and accumulation on meniscal tissue is frequently observed especially in elderly. In this study, we used X-ray diffraction (XRD), FTIR and FT-Raman for the structural identification of the depositions and Optical microscopy, Scanning Electron microscopy (SEM/EDX) and Atomic Force microscopy (AFM), in order to investigate the structural relationship between the crystal deposits and the collagen fibers of human meniscal tissues. We are reporting on the formation of intercalary “colonies” of Calcium Pyrophosphate Dihydrate (CPPD) crystals with two distinct morphologies corresponding to the monoclinic and the triclinic phase, as well as the formation of micro-aggregations composed of nano-crystalline HAP aggregations which are developed along the longitudinal axis of collagen fibers without extensively disturbing the collagens arrangement.
meniscal calcification, pathological mineralization, hydroxyapatite, calcium pyrophosphate dihydrate
1201-1209
Katsamenis, Orestis L.
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Karoutsos, Vagelis
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Kontostanos, Konstantinos
ab055ef9-965d-496b-8a51-56ac0e98fec8
Panagiotopoulos, Elias C.
91f90d97-2047-4fd2-9e29-2346da1982e8
Papadaki, Helen
6b41e1c8-bff2-45cd-a316-a3110248ab28
Bouropoulos, Nikolaos
fadadea1-2aa2-4cd9-a7e5-33ba790cd45f
November 2012
Katsamenis, Orestis L.
8553e7c3-d860-4b7a-a883-abf6c0c4b438
Karoutsos, Vagelis
64e29fc1-d119-4454-a4de-0f0572cde8bf
Kontostanos, Konstantinos
ab055ef9-965d-496b-8a51-56ac0e98fec8
Panagiotopoulos, Elias C.
91f90d97-2047-4fd2-9e29-2346da1982e8
Papadaki, Helen
6b41e1c8-bff2-45cd-a316-a3110248ab28
Bouropoulos, Nikolaos
fadadea1-2aa2-4cd9-a7e5-33ba790cd45f
Katsamenis, Orestis L., Karoutsos, Vagelis, Kontostanos, Konstantinos, Panagiotopoulos, Elias C., Papadaki, Helen and Bouropoulos, Nikolaos
(2012)
Microstructural characterization of CPPD and hydroxyapatite crystal depositions on human menisci.
Crystal Research and Technology, 47 (11), .
(doi:10.1002/crat.201200346).
Abstract
Meniscus is a fibrocartilaginous tissue composed mainly of water and a dense elaborate collagen network with a predominantly circumferential alignment. Crystal formation and accumulation on meniscal tissue is frequently observed especially in elderly. In this study, we used X-ray diffraction (XRD), FTIR and FT-Raman for the structural identification of the depositions and Optical microscopy, Scanning Electron microscopy (SEM/EDX) and Atomic Force microscopy (AFM), in order to investigate the structural relationship between the crystal deposits and the collagen fibers of human meniscal tissues. We are reporting on the formation of intercalary “colonies” of Calcium Pyrophosphate Dihydrate (CPPD) crystals with two distinct morphologies corresponding to the monoclinic and the triclinic phase, as well as the formation of micro-aggregations composed of nano-crystalline HAP aggregations which are developed along the longitudinal axis of collagen fibers without extensively disturbing the collagens arrangement.
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More information
Accepted/In Press date: 27 July 2012
e-pub ahead of print date: 23 August 2012
Published date: November 2012
Keywords:
meniscal calcification, pathological mineralization, hydroxyapatite, calcium pyrophosphate dihydrate
Organisations:
Engineering Mats & Surface Engineerg Gp
Identifiers
Local EPrints ID: 372303
URI: http://eprints.soton.ac.uk/id/eprint/372303
ISSN: 0232-1300
PURE UUID: 2d6bef0f-7d55-4965-9b83-db404d6fc75c
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Date deposited: 27 Jul 2015 10:30
Last modified: 15 Mar 2024 03:38
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Contributors
Author:
Vagelis Karoutsos
Author:
Konstantinos Kontostanos
Author:
Elias C. Panagiotopoulos
Author:
Helen Papadaki
Author:
Nikolaos Bouropoulos
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