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Nanomechanical assessment of human and murine collagen fibrils via atomic force microscopy cantilever-based nanoindentation

Nanomechanical assessment of human and murine collagen fibrils via atomic force microscopy cantilever-based nanoindentation
Nanomechanical assessment of human and murine collagen fibrils via atomic force microscopy cantilever-based nanoindentation
The nanomechanical assessment of collagen fibrils via atomic force microscopy (AFM) is of increasing interest within the biomedical research community. In contrast to conventional nanoindentation there exists no common standard for conducting experiments and analysis of data. Currently used analysis approaches vary between studies and validation of quantitative results is usually not performed, which makes comparison of data from different studies difficult. Also there are no recommendations with regards to the maximum indentation depth that should not be exceeded to avoid substrate effects. Here we present a methodology and analysis approach for AFM cantilever-based nanoindentation experiments that allows efficient use of captured data and relying on a reference sample for determination of tip shape. Further we show experimental evidence that maximum indentation depth on collagen fibrils should be lower than 10-15% of the height of the fibril to avoid substrate effects and we show comparisons between our and other approaches used in previous works. While our analysis approach yields similar values for indentation modulus compared to the Oliver-Pharr method we found that Hertzian analysis yielded significantly lower values. Applying our approach we successfully and efficiently indented collagen fibrils from human bronchi, which were about 30. nm in size, considerably smaller compared to collagen fibrils obtained from murine tail-tendon. In addition, derived mechanical parameters of collagen fibrils are in agreement with data previously published. To establish a quantitative validation we compared indentation results from conventional and AFM cantilever-based nanoindentation on polymeric samples with known mechanical properties. Importantly we can show that our approach yields similar results when compared to conventional nanoindentation on polymer samples. Introducing an approach that is reliable, efficient and taking into account the AFM tip shape, we anticipate that the present work may act as a guideline for conducting AFM cantilever-based nanoindentation of collagen fibrils. This may aid understanding of collagen-related diseases such as asthma, lung fibrosis or bone disease with potential alterations of collagen fibril mechanics. ?? 2014.
Airways, Atomic force microscopy, Bronchi, Collagen, Indentation, Mechanics, Mouse, Rat
1751-6161
9-26
Andriotis, Orestis G.
714f98eb-2fa3-4a97-99f9-f7e7765ec128
Manuyakorn, Wiparat
ad62b7a2-bb60-4a33-b37d-c1be767915af
Zekonyte, Jurgita
51de4316-3f4b-4a32-864f-fcfe1b510b17
Katsamenis, Orestis
8553e7c3-d860-4b7a-a883-abf6c0c4b438
Fabri, Sebastien
7af7faf9-d5c2-4e1b-864e-a0b633cf1e0f
Howarth, Peter H.
e96c434a-e6f0-4ff9-83e3-6727ce5b2490
Davies, Donna E.
7de8fdc7-3640-4e3a-aa91-d0e03f990c38
Thurner, Philipp J.
bafae261-c99f-4cee-aa19-3b333ef95af1
Andriotis, Orestis G.
714f98eb-2fa3-4a97-99f9-f7e7765ec128
Manuyakorn, Wiparat
ad62b7a2-bb60-4a33-b37d-c1be767915af
Zekonyte, Jurgita
51de4316-3f4b-4a32-864f-fcfe1b510b17
Katsamenis, Orestis
8553e7c3-d860-4b7a-a883-abf6c0c4b438
Fabri, Sebastien
7af7faf9-d5c2-4e1b-864e-a0b633cf1e0f
Howarth, Peter H.
e96c434a-e6f0-4ff9-83e3-6727ce5b2490
Davies, Donna E.
7de8fdc7-3640-4e3a-aa91-d0e03f990c38
Thurner, Philipp J.
bafae261-c99f-4cee-aa19-3b333ef95af1

Andriotis, Orestis G., Manuyakorn, Wiparat, Zekonyte, Jurgita, Katsamenis, Orestis, Fabri, Sebastien, Howarth, Peter H., Davies, Donna E. and Thurner, Philipp J. (2014) Nanomechanical assessment of human and murine collagen fibrils via atomic force microscopy cantilever-based nanoindentation. Journal of the Mechanical Behavior of Biomedical Materials, 39, 9-26. (doi:10.1016/j.jmbbm.2014.06.015).

Record type: Article

Abstract

The nanomechanical assessment of collagen fibrils via atomic force microscopy (AFM) is of increasing interest within the biomedical research community. In contrast to conventional nanoindentation there exists no common standard for conducting experiments and analysis of data. Currently used analysis approaches vary between studies and validation of quantitative results is usually not performed, which makes comparison of data from different studies difficult. Also there are no recommendations with regards to the maximum indentation depth that should not be exceeded to avoid substrate effects. Here we present a methodology and analysis approach for AFM cantilever-based nanoindentation experiments that allows efficient use of captured data and relying on a reference sample for determination of tip shape. Further we show experimental evidence that maximum indentation depth on collagen fibrils should be lower than 10-15% of the height of the fibril to avoid substrate effects and we show comparisons between our and other approaches used in previous works. While our analysis approach yields similar values for indentation modulus compared to the Oliver-Pharr method we found that Hertzian analysis yielded significantly lower values. Applying our approach we successfully and efficiently indented collagen fibrils from human bronchi, which were about 30. nm in size, considerably smaller compared to collagen fibrils obtained from murine tail-tendon. In addition, derived mechanical parameters of collagen fibrils are in agreement with data previously published. To establish a quantitative validation we compared indentation results from conventional and AFM cantilever-based nanoindentation on polymeric samples with known mechanical properties. Importantly we can show that our approach yields similar results when compared to conventional nanoindentation on polymer samples. Introducing an approach that is reliable, efficient and taking into account the AFM tip shape, we anticipate that the present work may act as a guideline for conducting AFM cantilever-based nanoindentation of collagen fibrils. This may aid understanding of collagen-related diseases such as asthma, lung fibrosis or bone disease with potential alterations of collagen fibril mechanics. ?? 2014.

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More information

Published date: 2014
Keywords: Airways, Atomic force microscopy, Bronchi, Collagen, Indentation, Mechanics, Mouse, Rat
Organisations: Bioengineering Group

Identifiers

Local EPrints ID: 372317
URI: http://eprints.soton.ac.uk/id/eprint/372317
ISSN: 1751-6161
PURE UUID: 679168f3-9e24-4597-a843-f19805332361
ORCID for Orestis Katsamenis: ORCID iD orcid.org/0000-0003-4367-4147
ORCID for Donna E. Davies: ORCID iD orcid.org/0000-0002-5117-2991

Catalogue record

Date deposited: 22 Jan 2015 09:12
Last modified: 18 Feb 2021 17:16

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