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Potentiation of tumor necrosis factor -induced secreted phospholipase A2 (sPLA2)-IIA expression in mesangial cells by an autocrine loop involving sPLA2 and peroxisome Proliferator-activated Receptor Activation

Potentiation of tumor necrosis factor -induced secreted phospholipase A2 (sPLA2)-IIA expression in mesangial cells by an autocrine loop involving sPLA2 and peroxisome Proliferator-activated Receptor Activation
Potentiation of tumor necrosis factor -induced secreted phospholipase A2 (sPLA2)-IIA expression in mesangial cells by an autocrine loop involving sPLA2 and peroxisome Proliferator-activated Receptor Activation
In rat mesangial cells, exogenously added secreted phospholipases A2 (sPLA2s) potentiate the expression of pro-inflammatory sPLA2-IIA first induced by cytokines like tumor necrosis factor-? (TNF?) and interleukin-1?. The transcriptional pathway mediating this effect is, however, unknown. Because products of PLA2 activity are endogenous activators of peroxisome proliferator-activated receptor ? (PPAR?, we postulated that sPLA2s mediate their effects on sPLA2-IIA expression via sPLA2 activity and subsequent PPAR? activation. This study shows that various sPLA2s, including venom enzymes, human sPLA2-IIA, and wild-type and catalytically inactive H48Q mutant of porcine pancreatic sPLA2-IB, enhance the TNF?-induced sPLA2-IIA expression at the mRNA and protein levels. In cells transfected with luciferase sPLA2-IIA promoter constructs, sPLA2s are active only when the promoter contains a functional PPRE-1 site. The effect of exogenous sPLA2s is also blocked by the PPAR? inhibitor MK886. Interestingly, the expression of sPLA2-IIA induced by TNF? alone is also attenuated by MK886, by the sPLA2-IIA inhibitor LY311727, by heparinase, which prevents the binding of sPLA2-IIA to heparan sulfate proteoglycans, and by the specific cPLA2-? inhibitor pyrrolidine-1. Together, these data indicate that sPLA2-IIA released from mesangial cells by TNF? stimulates its own expression via an autocrine loop involving cPLA2 and PPAR?. This signaling pathway is also used by exogenously added sPLA2s including pancreatic sPLA2-IB and is distinct from that used by TNF?.
0021-9258
29799-29812
Beck, S.
67ec6ea3-2aed-415b-894b-55eefb959479
Lambeau, G.
5e99c084-e370-4270-a133-8d771ee57334
Scholz-Pedretti, K.
51aee60f-4642-44f9-84e3-90273d7d3372
Gelb, M.H.
be15b679-9672-486f-8d02-3eb65da4dad2
Janssen, M.J.W.
f74418dc-a155-4399-8747-56f85571424d
Edwards, S.H.
bc84297b-f5fa-4456-9b10-5c363cb4d057
Wilton, D.C.
4b995f66-ad6c-4d96-9179-c64f3b54466a
Pfeilschifter, J.
39a39944-567b-47d5-8973-630dcd32b2cc
Kaszkin, M.
69887853-3976-448e-bf7f-4d53ee3184ff
Beck, S.
67ec6ea3-2aed-415b-894b-55eefb959479
Lambeau, G.
5e99c084-e370-4270-a133-8d771ee57334
Scholz-Pedretti, K.
51aee60f-4642-44f9-84e3-90273d7d3372
Gelb, M.H.
be15b679-9672-486f-8d02-3eb65da4dad2
Janssen, M.J.W.
f74418dc-a155-4399-8747-56f85571424d
Edwards, S.H.
bc84297b-f5fa-4456-9b10-5c363cb4d057
Wilton, D.C.
4b995f66-ad6c-4d96-9179-c64f3b54466a
Pfeilschifter, J.
39a39944-567b-47d5-8973-630dcd32b2cc
Kaszkin, M.
69887853-3976-448e-bf7f-4d53ee3184ff

Beck, S., Lambeau, G., Scholz-Pedretti, K., Gelb, M.H., Janssen, M.J.W., Edwards, S.H., Wilton, D.C., Pfeilschifter, J. and Kaszkin, M. (2003) Potentiation of tumor necrosis factor -induced secreted phospholipase A2 (sPLA2)-IIA expression in mesangial cells by an autocrine loop involving sPLA2 and peroxisome Proliferator-activated Receptor Activation. The Journal of Biological Chemistry, 278 (32), 29799-29812. (doi:10.1074/jbc.M211763200).

Record type: Article

Abstract

In rat mesangial cells, exogenously added secreted phospholipases A2 (sPLA2s) potentiate the expression of pro-inflammatory sPLA2-IIA first induced by cytokines like tumor necrosis factor-? (TNF?) and interleukin-1?. The transcriptional pathway mediating this effect is, however, unknown. Because products of PLA2 activity are endogenous activators of peroxisome proliferator-activated receptor ? (PPAR?, we postulated that sPLA2s mediate their effects on sPLA2-IIA expression via sPLA2 activity and subsequent PPAR? activation. This study shows that various sPLA2s, including venom enzymes, human sPLA2-IIA, and wild-type and catalytically inactive H48Q mutant of porcine pancreatic sPLA2-IB, enhance the TNF?-induced sPLA2-IIA expression at the mRNA and protein levels. In cells transfected with luciferase sPLA2-IIA promoter constructs, sPLA2s are active only when the promoter contains a functional PPRE-1 site. The effect of exogenous sPLA2s is also blocked by the PPAR? inhibitor MK886. Interestingly, the expression of sPLA2-IIA induced by TNF? alone is also attenuated by MK886, by the sPLA2-IIA inhibitor LY311727, by heparinase, which prevents the binding of sPLA2-IIA to heparan sulfate proteoglycans, and by the specific cPLA2-? inhibitor pyrrolidine-1. Together, these data indicate that sPLA2-IIA released from mesangial cells by TNF? stimulates its own expression via an autocrine loop involving cPLA2 and PPAR?. This signaling pathway is also used by exogenously added sPLA2s including pancreatic sPLA2-IB and is distinct from that used by TNF?.

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Published date: August 2003
Organisations: Centre for Biological Sciences

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Local EPrints ID: 372326
URI: http://eprints.soton.ac.uk/id/eprint/372326
ISSN: 0021-9258
PURE UUID: 21e5486e-d131-45ed-805a-fb2771f49153

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Date deposited: 01 Dec 2014 11:38
Last modified: 15 Jul 2019 21:37

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Contributors

Author: S. Beck
Author: G. Lambeau
Author: K. Scholz-Pedretti
Author: M.H. Gelb
Author: M.J.W. Janssen
Author: S.H. Edwards
Author: D.C. Wilton
Author: J. Pfeilschifter
Author: M. Kaszkin

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