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Dynamics of microvascular blood flow and oxygenation measured simultaneously in human skin

Dynamics of microvascular blood flow and oxygenation measured simultaneously in human skin
Dynamics of microvascular blood flow and oxygenation measured simultaneously in human skin
Objective: to evaluate the dynamics of skin microvascular blood flow (BF) and tissue oxygenation parameters (OXY) measured simultaneously at the same site using a combined non-invasive BF+OXY+temperature probe.

Methods: skin BF, oxygenated (oxyHb) and deoxygenated (deoxyHb) haemoglobin and mean oxygen saturation (SO2) were measured in 50 healthy volunteers at rest and during perturbation of local blood flow by post-occlusive reactive hyperaemia, sympathetic nervous system-mediated vasoconstriction (deep inspiratory breath-hold) and local skin warming. Signals were analysed in time and frequency domains.

Results: the relationship between BF and SO2 over the range of flows investigated was described by a non-linear equation with an asymptote for SO2 of 84% at BF >50 PU. SO2 was independently associated with BF, skin temperature, BMI and age, which together identified 59% of the variance in SO2 (p<0.0001). Fourier analysis revealed periodic low frequency fluctuations in both BF and SO2, attributable to endothelial (~0.01 Hz), neurogenic (~0.04 Hz) and myogenic (~0.1Hz) flow motion activity. The frequency coherence between the BF and SO2 signals was greatest in the endothelial and neurogenic frequency bands.

Conclusions: the simultaneous evaluation of microvascular blood flow and oxygenation kinetics in healthy skin provides a platform from which to investigate microvascular impairment in the skin and more generally the pathogenesis of microvascular disease
1073-9688
562-573
Kuliga, Katarzyna Z.
654cffbb-2d3c-4b15-9c99-2e89bda1bca3
McDonald, Erin F.
f36faec7-72c4-49ee-8ca0-426e48901f5c
Gush, Rodney
cfdfe7f0-88c8-4f76-ae3a-e37a73eb04c5
Michel, Charles
a857bae8-f25d-43e9-96af-9c36209302fc
Chipperfield, A.J.
524269cd-5f30-4356-92d4-891c14c09340
Clough, G.F.
9f19639e-a929-4976-ac35-259f9011c494
Kuliga, Katarzyna Z.
654cffbb-2d3c-4b15-9c99-2e89bda1bca3
McDonald, Erin F.
f36faec7-72c4-49ee-8ca0-426e48901f5c
Gush, Rodney
cfdfe7f0-88c8-4f76-ae3a-e37a73eb04c5
Michel, Charles
a857bae8-f25d-43e9-96af-9c36209302fc
Chipperfield, A.J.
524269cd-5f30-4356-92d4-891c14c09340
Clough, G.F.
9f19639e-a929-4976-ac35-259f9011c494

Kuliga, Katarzyna Z., McDonald, Erin F., Gush, Rodney, Michel, Charles, Chipperfield, A.J. and Clough, G.F. (2014) Dynamics of microvascular blood flow and oxygenation measured simultaneously in human skin. Microcirculation, 21 (6), 562-573. (doi:10.1111/micc.12136). (PMID:24689754)

Record type: Article

Abstract

Objective: to evaluate the dynamics of skin microvascular blood flow (BF) and tissue oxygenation parameters (OXY) measured simultaneously at the same site using a combined non-invasive BF+OXY+temperature probe.

Methods: skin BF, oxygenated (oxyHb) and deoxygenated (deoxyHb) haemoglobin and mean oxygen saturation (SO2) were measured in 50 healthy volunteers at rest and during perturbation of local blood flow by post-occlusive reactive hyperaemia, sympathetic nervous system-mediated vasoconstriction (deep inspiratory breath-hold) and local skin warming. Signals were analysed in time and frequency domains.

Results: the relationship between BF and SO2 over the range of flows investigated was described by a non-linear equation with an asymptote for SO2 of 84% at BF >50 PU. SO2 was independently associated with BF, skin temperature, BMI and age, which together identified 59% of the variance in SO2 (p<0.0001). Fourier analysis revealed periodic low frequency fluctuations in both BF and SO2, attributable to endothelial (~0.01 Hz), neurogenic (~0.04 Hz) and myogenic (~0.1Hz) flow motion activity. The frequency coherence between the BF and SO2 signals was greatest in the endothelial and neurogenic frequency bands.

Conclusions: the simultaneous evaluation of microvascular blood flow and oxygenation kinetics in healthy skin provides a platform from which to investigate microvascular impairment in the skin and more generally the pathogenesis of microvascular disease

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More information

e-pub ahead of print date: 6 August 2014
Published date: 6 August 2014
Organisations: Bioengineering Group, Human Development & Health

Identifiers

Local EPrints ID: 372360
URI: http://eprints.soton.ac.uk/id/eprint/372360
ISSN: 1073-9688
PURE UUID: 8e75a9d1-026b-40a4-85fc-7057b219e79e
ORCID for A.J. Chipperfield: ORCID iD orcid.org/0000-0002-3026-9890
ORCID for G.F. Clough: ORCID iD orcid.org/0000-0002-6226-8964

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Date deposited: 08 Dec 2014 14:37
Last modified: 29 Oct 2019 02:03

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Contributors

Author: Katarzyna Z. Kuliga
Author: Erin F. McDonald
Author: Rodney Gush
Author: Charles Michel
Author: G.F. Clough ORCID iD

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