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Manipulation and trapping of sub-micron bioparticles using dielectrophoresis

Manipulation and trapping of sub-micron bioparticles using dielectrophoresis
Manipulation and trapping of sub-micron bioparticles using dielectrophoresis
A non-uniform alternating electric field induces motion in polarisable particles called dielectrophoresis. The effect is governed by the relative magnitudes of the dielectric properties of the medium and the particles. The technology has been used to manipulate particles for biotechnological applications, including purification, fractionation and concentration of cells and micro-organisms. However, the lower size limit for the dielectrophoretic manipulation of particles was believed to be about 1 ?m, but recent work has proved otherwise. The dielectrophoretic movement and properties of latex beads and a simple rod-shaped virus, tobacco mosaic virus (TMV), have been measured using microfabricated electrode structures. Measurements have been made over a range of suspending medium conductivities, applied frequencies and electric field strengths. It is shown that under appropriate conditions both latex beads and tobacco mosaic virus particles can be selectively attracted to regions of high electric field strength located at the tips of microfabricated electrode structures. The ability to selectively trap and separate bio-particles has many potential applications in the area of biotechnology.
0165-022X
89-102
Green, Nicolas G
d9b47269-c426-41fd-a41d-5f4579faa581
Morgan, Hywel
de00d59f-a5a2-48c4-a99a-1d5dd7854174
Milner, J J
a3aa835e-f63b-4b00-aa21-3ac630a4f270
Green, Nicolas G
d9b47269-c426-41fd-a41d-5f4579faa581
Morgan, Hywel
de00d59f-a5a2-48c4-a99a-1d5dd7854174
Milner, J J
a3aa835e-f63b-4b00-aa21-3ac630a4f270

Green, Nicolas G, Morgan, Hywel and Milner, J J (1997) Manipulation and trapping of sub-micron bioparticles using dielectrophoresis. Journal of Biochemical and Biophysical Methods, 35 (2), 89-102. (doi:10.1016/S0165-022X(97)00033-X).

Record type: Article

Abstract

A non-uniform alternating electric field induces motion in polarisable particles called dielectrophoresis. The effect is governed by the relative magnitudes of the dielectric properties of the medium and the particles. The technology has been used to manipulate particles for biotechnological applications, including purification, fractionation and concentration of cells and micro-organisms. However, the lower size limit for the dielectrophoretic manipulation of particles was believed to be about 1 ?m, but recent work has proved otherwise. The dielectrophoretic movement and properties of latex beads and a simple rod-shaped virus, tobacco mosaic virus (TMV), have been measured using microfabricated electrode structures. Measurements have been made over a range of suspending medium conductivities, applied frequencies and electric field strengths. It is shown that under appropriate conditions both latex beads and tobacco mosaic virus particles can be selectively attracted to regions of high electric field strength located at the tips of microfabricated electrode structures. The ability to selectively trap and separate bio-particles has many potential applications in the area of biotechnology.

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J4_Green_Morgan_Milner_J_Biochemical_Biophysical_Methods_1997.pdf - Other
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Published date: 25 September 1997
Organisations: Electronics & Computer Science

Identifiers

Local EPrints ID: 372365
URI: https://eprints.soton.ac.uk/id/eprint/372365
ISSN: 0165-022X
PURE UUID: b71b0d3b-93b1-404e-974b-27051aca87f9
ORCID for Nicolas G Green: ORCID iD orcid.org/0000-0001-9230-4455
ORCID for Hywel Morgan: ORCID iD orcid.org/0000-0003-4850-5676

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Date deposited: 01 Dec 2014 16:37
Last modified: 07 Aug 2019 00:43

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Contributors

Author: Nicolas G Green ORCID iD
Author: Hywel Morgan ORCID iD
Author: J J Milner

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