The University of Southampton
University of Southampton Institutional Repository

Separation of Submicron Bioparticles by Dielectrophoresis

Separation of Submicron Bioparticles by Dielectrophoresis
Separation of Submicron Bioparticles by Dielectrophoresis
Submicron particles such as latex spheres and viruses can be manipulated and characterized using dielectrophoresis. By the use of appropriate microelectrode arrays, particles can be trapped or moved between regions of high or low electric fields. The magnitude and direction of the dielectrophoretic force on the particle depends on its dielectric properties, so that a heterogeneous mixture of particles can be separated to produce a more homogeneous population. In this paper the controlled separation of submicron bioparticles is demonstrated. With electrode arrays fabricated using direct write electron beam lithography, it is shown that different types of submicron latex spheres can be spatially separated. The separation occurs as a result of differences in magnitude and/or direction of the dielectrophoretic force on different populations of particles. These differences arise mainly because the surface properties of submicron particles dominate their dielectrophoretic behavior. It is also demonstrated that tobacco mosaic virus and herpes simplex virus can be manipulated and spatially separated in a microelectrode array.
0006-3495
516-525
Morgan, Hywel
de00d59f-a5a2-48c4-a99a-1d5dd7854174
Hughes, Michael P
b72a2cbb-7780-4910-be78-189d07986ed9
Green, Nicolas G
d9b47269-c426-41fd-a41d-5f4579faa581
Morgan, Hywel
de00d59f-a5a2-48c4-a99a-1d5dd7854174
Hughes, Michael P
b72a2cbb-7780-4910-be78-189d07986ed9
Green, Nicolas G
d9b47269-c426-41fd-a41d-5f4579faa581

Morgan, Hywel, Hughes, Michael P and Green, Nicolas G (1999) Separation of Submicron Bioparticles by Dielectrophoresis. Biophysical Journal, 77 (1), 516-525. (doi:10.1016/S0006-3495(99)76908-0).

Record type: Article

Abstract

Submicron particles such as latex spheres and viruses can be manipulated and characterized using dielectrophoresis. By the use of appropriate microelectrode arrays, particles can be trapped or moved between regions of high or low electric fields. The magnitude and direction of the dielectrophoretic force on the particle depends on its dielectric properties, so that a heterogeneous mixture of particles can be separated to produce a more homogeneous population. In this paper the controlled separation of submicron bioparticles is demonstrated. With electrode arrays fabricated using direct write electron beam lithography, it is shown that different types of submicron latex spheres can be spatially separated. The separation occurs as a result of differences in magnitude and/or direction of the dielectrophoretic force on different populations of particles. These differences arise mainly because the surface properties of submicron particles dominate their dielectrophoretic behavior. It is also demonstrated that tobacco mosaic virus and herpes simplex virus can be manipulated and spatially separated in a microelectrode array.

Text
J10_Morgan_Hughes_Green_Biophysical_Journal_1999.pdf - Other
Download (735kB)

More information

Published date: July 1999
Organisations: Electronics & Computer Science

Identifiers

Local EPrints ID: 372371
URI: https://eprints.soton.ac.uk/id/eprint/372371
ISSN: 0006-3495
PURE UUID: 12cfeb1d-a95e-4cfe-8f72-d30dedf005cc
ORCID for Hywel Morgan: ORCID iD orcid.org/0000-0003-4850-5676
ORCID for Nicolas G Green: ORCID iD orcid.org/0000-0001-9230-4455

Catalogue record

Date deposited: 01 Dec 2014 17:41
Last modified: 17 Sep 2019 00:52

Export record

Altmetrics

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of https://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×