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An optoelectrokinetic technique for programmable particle manipulation and bead-based biosignal enhancement

An optoelectrokinetic technique for programmable particle manipulation and bead-based biosignal enhancement
An optoelectrokinetic technique for programmable particle manipulation and bead-based biosignal enhancement
Technologies that can enable concentration of low-abundance biomarkers are essential for early diagnosis of diseases. In this study, an optoelectrokinetic technique, termed Rapid Electrokinetic Patterning (REP), was used to enable dynamic particle manipulation in bead-based bioassays. Various manipulation capabilities, such as micro/nanoparticle aggregation, translation, sorting and patterning, were developed. The technique allows for versatile multi-parameter (voltage, light intensity and frequency) based modulation and dynamically addressable manipulation with simple device fabrication. Signal enhancement of a bead-based bioassay was demonstrated using dilute biotin–fluorescein isothiocyanate (FITC) solutions mixed with streptavidin-conjugated particles and rapidly concentrated with the technique. As compared with a conventional ELISA reader, the REP-enabled detection achieved a minimal readout of 3.87 nM, which was a 100-fold improvement in sensitivity. The multi-functional platform provides an effective measure to enhance detection levels in more bead-based bioassays.
1473-0197
3958-3967
Wang, Kuan-Chih
3ba15aae-311c-4285-8e7d-c6266cf7783b
Kumar, Aloke
8e9992d8-c15e-456c-91ca-e78cd39f553b
Williams, Stuart J
a737262a-289a-42ab-bb5b-777571ff6203
Green, Nicolas G
d9b47269-c426-41fd-a41d-5f4579faa581
Kim, Kyung Chun
ebe34fab-a8a8-4930-8a4b-8750d6a602ff
Chuang, Han-Sheng
8760db2f-9ffd-4493-85a9-8e9e1969840c
Wang, Kuan-Chih
3ba15aae-311c-4285-8e7d-c6266cf7783b
Kumar, Aloke
8e9992d8-c15e-456c-91ca-e78cd39f553b
Williams, Stuart J
a737262a-289a-42ab-bb5b-777571ff6203
Green, Nicolas G
d9b47269-c426-41fd-a41d-5f4579faa581
Kim, Kyung Chun
ebe34fab-a8a8-4930-8a4b-8750d6a602ff
Chuang, Han-Sheng
8760db2f-9ffd-4493-85a9-8e9e1969840c

Wang, Kuan-Chih, Kumar, Aloke, Williams, Stuart J, Green, Nicolas G, Kim, Kyung Chun and Chuang, Han-Sheng (2014) An optoelectrokinetic technique for programmable particle manipulation and bead-based biosignal enhancement. Lab on a Chip, 14, 3958-3967. (doi:10.1039/C4LC00661E).

Record type: Article

Abstract

Technologies that can enable concentration of low-abundance biomarkers are essential for early diagnosis of diseases. In this study, an optoelectrokinetic technique, termed Rapid Electrokinetic Patterning (REP), was used to enable dynamic particle manipulation in bead-based bioassays. Various manipulation capabilities, such as micro/nanoparticle aggregation, translation, sorting and patterning, were developed. The technique allows for versatile multi-parameter (voltage, light intensity and frequency) based modulation and dynamically addressable manipulation with simple device fabrication. Signal enhancement of a bead-based bioassay was demonstrated using dilute biotin–fluorescein isothiocyanate (FITC) solutions mixed with streptavidin-conjugated particles and rapidly concentrated with the technique. As compared with a conventional ELISA reader, the REP-enabled detection achieved a minimal readout of 3.87 nM, which was a 100-fold improvement in sensitivity. The multi-functional platform provides an effective measure to enhance detection levels in more bead-based bioassays.

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e-pub ahead of print date: 2014
Published date: 11 August 2014
Organisations: Electronics & Computer Science

Identifiers

Local EPrints ID: 372430
URI: https://eprints.soton.ac.uk/id/eprint/372430
ISSN: 1473-0197
PURE UUID: 8c56012d-389a-455d-b566-fc525a0c349d
ORCID for Nicolas G Green: ORCID iD orcid.org/0000-0001-9230-4455

Catalogue record

Date deposited: 03 Dec 2014 13:46
Last modified: 03 Dec 2019 01:48

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Contributors

Author: Kuan-Chih Wang
Author: Aloke Kumar
Author: Stuart J Williams
Author: Nicolas G Green ORCID iD
Author: Kyung Chun Kim
Author: Han-Sheng Chuang

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