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The relative orientation of the fibronectin 6F11F2 module pair: A 15N NMR relaxation study

The relative orientation of the fibronectin 6F11F2 module pair: A 15N NMR relaxation study
The relative orientation of the fibronectin 6F11F2 module pair: A 15N NMR relaxation study
The structure of a pair of modules (6F11F2), that forms part of the collagen-binding region of fibronectin, is refined using heteronuclear relaxation data. A structure of the pair was previously derived from 1H-1H NOE and 3 J H?HN data [Bocquier et al. (1999) Structure, 7, 1451–1460] and a weak module–module interface, comprising Leu19 and Leu28, in 6F1, and Tyr68 in 2F1, was identified. In this study, the definition of the average relative orientation of the two modules is improved using the dependence of 15N relaxation on rotational diffusion anisotropy. This structure refinement is based on the selection of a subset of structures from sets calculated with NOE and 3 J H?HN data alone, using the quality of the fits to the relaxation data as the selection criterion. This simple approach is compared to a refinement strategy where 15N relaxation data are included in the force field as additional restraints [Tjandra et al. (1997) Nat. Struct. Biol., 4, 443–449]
anisotropic diffusion, collagen-binding, fibronectin, module, 15N relaxation
0925-2738
203-214
Hashimoto, Yasuhiro
c0b5dbef-73f6-4d02-a7b4-14e409039c53
Smith, Steven P.
663cd8fd-14b7-4e79-b774-8c7817c2cf42
Pickford, Andrew R.
a44df69d-1790-43fa-9c04-5d79351e9ab9
Bocquier, Arnaud A.
d76be157-f810-4a08-b89d-dc7a0ee08ab2
Campbell, Iain D.
54eba33e-94f7-450e-b555-c429dc2179de
Werner, Jörn M.
1b02513a-8310-4f4f-adac-dc2a466bd115
Hashimoto, Yasuhiro
c0b5dbef-73f6-4d02-a7b4-14e409039c53
Smith, Steven P.
663cd8fd-14b7-4e79-b774-8c7817c2cf42
Pickford, Andrew R.
a44df69d-1790-43fa-9c04-5d79351e9ab9
Bocquier, Arnaud A.
d76be157-f810-4a08-b89d-dc7a0ee08ab2
Campbell, Iain D.
54eba33e-94f7-450e-b555-c429dc2179de
Werner, Jörn M.
1b02513a-8310-4f4f-adac-dc2a466bd115

Hashimoto, Yasuhiro, Smith, Steven P., Pickford, Andrew R., Bocquier, Arnaud A., Campbell, Iain D. and Werner, Jörn M. (2000) The relative orientation of the fibronectin 6F11F2 module pair: A 15N NMR relaxation study. Journal of Biomolecular NMR, 17 (3), 203-214. (doi:10.1023/A:1008341609461).

Record type: Article

Abstract

The structure of a pair of modules (6F11F2), that forms part of the collagen-binding region of fibronectin, is refined using heteronuclear relaxation data. A structure of the pair was previously derived from 1H-1H NOE and 3 J H?HN data [Bocquier et al. (1999) Structure, 7, 1451–1460] and a weak module–module interface, comprising Leu19 and Leu28, in 6F1, and Tyr68 in 2F1, was identified. In this study, the definition of the average relative orientation of the two modules is improved using the dependence of 15N relaxation on rotational diffusion anisotropy. This structure refinement is based on the selection of a subset of structures from sets calculated with NOE and 3 J H?HN data alone, using the quality of the fits to the relaxation data as the selection criterion. This simple approach is compared to a refinement strategy where 15N relaxation data are included in the force field as additional restraints [Tjandra et al. (1997) Nat. Struct. Biol., 4, 443–449]

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More information

Published date: 2000
Keywords: anisotropic diffusion, collagen-binding, fibronectin, module, 15N relaxation
Organisations: Centre for Biological Sciences

Identifiers

Local EPrints ID: 372531
URI: https://eprints.soton.ac.uk/id/eprint/372531
ISSN: 0925-2738
PURE UUID: 3a54ad35-9023-49a9-954b-d0289b93bf9b
ORCID for Jörn M. Werner: ORCID iD orcid.org/0000-0002-4712-1833

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Date deposited: 08 Dec 2014 12:08
Last modified: 06 Jun 2018 12:45

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