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Effects of amino acid antagonists on spontaneous dorsal root activity and evoked dorsal horn field potentials in an isolated preparation of rat spinal cord

Effects of amino acid antagonists on spontaneous dorsal root activity and evoked dorsal horn field potentials in an isolated preparation of rat spinal cord
Effects of amino acid antagonists on spontaneous dorsal root activity and evoked dorsal horn field potentials in an isolated preparation of rat spinal cord
Fast and slow dorsal horn field potentials and spontaneous dorsal root activity were recorded from 19-23-day-old rat isolated spinal cord preparations. The effects of GABA, glycine, and glutamate antagonists were tested on these recordings. CNQX, an AMPA/kainate antagonist, reduced all 3 components of the dorsal horn field potential whereas MK801, an NMDA ion channel antagonist, reduced the fast S2 component and the slow wave. Both reduced spontaneous dorsal root activity. NMDA antagonists, D-AP5, 7-chlorokynurenic acid and arcaine, and the metabotropic glutamate antagonists L-AP3 and ethylglutamic acid, while having little effect on the fast components of the field potential, all reduced the slow component. The GABA antagonist, bicuculline, and the glycine antagonist, strychnine, while having no effect on the fast S1 and slow components of the field potential, reduced both the fast S2 component of the field potential and spontaneous dorsal root activity. These results suggest that non-NMDA glutamate receptors are involved in low and high threshold transmission to dorsal horn neurones while NMDA and metabotropic glutamate receptors are primarily involved in high threshold transmission and both GABA and glycine have roles in the transmission or modulation of sensory information within the dorsal horn of the cord.
bicuculline, cnqx, dorsal horn potentials, dorsal root activity, isolated spinal cord, mk801, strychnine
0020-7454
85-106
Maile, R.A.
aaf8cd4b-5485-45f0-b98d-943f0c03a4da
Morgan, E.
c47b6e1b-3e83-4220-83cb-1a3bca539233
Bagust, J.
d746f6e0-788c-416f-8165-9a09eb8e08c9
Walker, R.J.
9368ac2d-f1e9-4bd9-a4b4-4a161c4aa140
Maile, R.A.
aaf8cd4b-5485-45f0-b98d-943f0c03a4da
Morgan, E.
c47b6e1b-3e83-4220-83cb-1a3bca539233
Bagust, J.
d746f6e0-788c-416f-8165-9a09eb8e08c9
Walker, R.J.
9368ac2d-f1e9-4bd9-a4b4-4a161c4aa140

Maile, R.A., Morgan, E., Bagust, J. and Walker, R.J. (2007) Effects of amino acid antagonists on spontaneous dorsal root activity and evoked dorsal horn field potentials in an isolated preparation of rat spinal cord. International Journal of Neuroscience, 117 (1), 85-106. (doi:10.1080/00207450500534217). (PMID:17365101)

Record type: Article

Abstract

Fast and slow dorsal horn field potentials and spontaneous dorsal root activity were recorded from 19-23-day-old rat isolated spinal cord preparations. The effects of GABA, glycine, and glutamate antagonists were tested on these recordings. CNQX, an AMPA/kainate antagonist, reduced all 3 components of the dorsal horn field potential whereas MK801, an NMDA ion channel antagonist, reduced the fast S2 component and the slow wave. Both reduced spontaneous dorsal root activity. NMDA antagonists, D-AP5, 7-chlorokynurenic acid and arcaine, and the metabotropic glutamate antagonists L-AP3 and ethylglutamic acid, while having little effect on the fast components of the field potential, all reduced the slow component. The GABA antagonist, bicuculline, and the glycine antagonist, strychnine, while having no effect on the fast S1 and slow components of the field potential, reduced both the fast S2 component of the field potential and spontaneous dorsal root activity. These results suggest that non-NMDA glutamate receptors are involved in low and high threshold transmission to dorsal horn neurones while NMDA and metabotropic glutamate receptors are primarily involved in high threshold transmission and both GABA and glycine have roles in the transmission or modulation of sensory information within the dorsal horn of the cord.

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More information

Published date: January 2007
Keywords: bicuculline, cnqx, dorsal horn potentials, dorsal root activity, isolated spinal cord, mk801, strychnine
Organisations: Centre for Biological Sciences

Identifiers

Local EPrints ID: 372563
URI: http://eprints.soton.ac.uk/id/eprint/372563
ISSN: 0020-7454
PURE UUID: 6251ff97-6558-4504-98e0-8ca46c9a89cd
ORCID for R.J. Walker: ORCID iD orcid.org/0000-0002-9031-7671

Catalogue record

Date deposited: 09 Dec 2014 10:35
Last modified: 20 Jul 2019 00:22

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