Endocytosis as a mode to regulate functional expression of two-pore domain potassium (K2P) channels
Endocytosis as a mode to regulate functional expression of two-pore domain potassium (K2P) channels
Two-pore domain potassium (K2P) channels are implicated in an array of physiological and pathophysiological roles. As a result of their biophysical properties, these channels produce a background leak K(+) current which has a direct effect on cellular membrane potential and activity. The regulation of potassium leak from cells through K2P channels is of critical importance to cell function, development and survival. Controlling the cell surface expression of these channels is one mode to regulate their function and is achieved through a balance between regulated channel delivery to and retrieval from the cell surface. Here, we explore the modes of retrieval of K2P channels from the plasma membrane and observe that K2P channels are endocytosed in both a clathrin-mediated and clathrin-independent manner. K2P channels use a variety of pathways and show altered internalisation and sorting in response to external cues. These pathways working in concert, equip the cell with a range of approaches to maintain steady state levels of channels and to respond rapidly should changes in channel density be required.
k2p channel, endocytosis, recycling, clathrin, twik, trek, task, tresk, potassium channel
1133-1142
O'Kelly, Ita
e640f28a-42f0-48a6-9ce2-cb5a85d08c66
May 2015
O'Kelly, Ita
e640f28a-42f0-48a6-9ce2-cb5a85d08c66
O'Kelly, Ita
(2015)
Endocytosis as a mode to regulate functional expression of two-pore domain potassium (K2P) channels.
Pflugers Archiv - European Journal of Physiology, 467 (5), .
(doi:10.1007/s00424-014-1641-9).
(PMID:25413469)
Abstract
Two-pore domain potassium (K2P) channels are implicated in an array of physiological and pathophysiological roles. As a result of their biophysical properties, these channels produce a background leak K(+) current which has a direct effect on cellular membrane potential and activity. The regulation of potassium leak from cells through K2P channels is of critical importance to cell function, development and survival. Controlling the cell surface expression of these channels is one mode to regulate their function and is achieved through a balance between regulated channel delivery to and retrieval from the cell surface. Here, we explore the modes of retrieval of K2P channels from the plasma membrane and observe that K2P channels are endocytosed in both a clathrin-mediated and clathrin-independent manner. K2P channels use a variety of pathways and show altered internalisation and sorting in response to external cues. These pathways working in concert, equip the cell with a range of approaches to maintain steady state levels of channels and to respond rapidly should changes in channel density be required.
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e-pub ahead of print date: 22 November 2014
Published date: May 2015
Keywords:
k2p channel, endocytosis, recycling, clathrin, twik, trek, task, tresk, potassium channel
Organisations:
Human Development & Health
Identifiers
Local EPrints ID: 372667
URI: http://eprints.soton.ac.uk/id/eprint/372667
ISSN: 0031-6768
PURE UUID: 99e4eab5-1a80-46ca-81b6-9fb2d31ffb64
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Date deposited: 17 Dec 2014 10:52
Last modified: 14 Mar 2024 18:40
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Author:
Ita O'Kelly
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