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Linking regional variation of epibiotic bacterial diversity and trophic ecology in a new species of Kiwaidae (Decapoda, Anomura) from East Scotia Ridge (Antarctica) hydrothermal vents

Linking regional variation of epibiotic bacterial diversity and trophic ecology in a new species of Kiwaidae (Decapoda, Anomura) from East Scotia Ridge (Antarctica) hydrothermal vents
Linking regional variation of epibiotic bacterial diversity and trophic ecology in a new species of Kiwaidae (Decapoda, Anomura) from East Scotia Ridge (Antarctica) hydrothermal vents
We analyzed the diversity of bacterial epibionts and trophic ecology of a new species of Kiwa yeti crab discovered at two hydrothermal vent fields (E2 and E9) on the East Scotia Ridge (ESR) in the Southern Ocean using a combination of 454 pyrosequencing, Sanger sequencing, and stable isotope analysis. The Kiwa epibiont communities were dominated by Epsilon- and Gammaproteobacteria. About 454 sequencing of the epibionts on 15 individual Kiwa specimen revealed large regional differences between the two hydrothermal vent fields: at E2, the bacterial community on the Kiwa ventral setae was dominated (up to 75%) by Gammaproteobacteria, whereas at E9 Epsilonproteobacteria dominated (up to 98%). Carbon stable isotope analysis of both Kiwa and the bacterial epibionts also showed distinct differences between E2 and E9 in mean and variability. Both stable isotope and sequence data suggest a dominance of different carbon fixation pathways of the epibiont communities at the two vent fields. At E2, epibionts were putatively fixing carbon via the Calvin-Benson-Bassham and reverse tricarboxylic acid cycle, while at E9 the reverse tricarboxylic acid cycle dominated. Co-varying epibiont diversity and isotope values at E2 and E9 also present further support for the hypothesis that epibionts serve as a food source for Kiwa.
East Scotia Ridge, epibionts, hydrothermal vent, Kiwa sp., microbial diversity, stable isotopes
2045-8827
136-150
Zwirglmaier, Katrin
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Reid, William D. K.
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Heywood, Jane
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Sweeting, Christopher J.
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Wigham, Benjamin D.
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Polunin, Nicholas V. C.
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Hawkes, Jeff A.
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Connelly, Douglas P.
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Pearce, David
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Linse, Katrin
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Zwirglmaier, Katrin
bab5e123-33f1-4b50-8d94-a34d438a5826
Reid, William D. K.
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Heywood, Jane
cbf4b7cd-680d-4ffd-8702-254848779673
Sweeting, Christopher J.
71971132-559e-49aa-837d-b003cbbc748a
Wigham, Benjamin D.
01711c28-8b86-4e1d-bade-fba8ede0a633
Polunin, Nicholas V. C.
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Hawkes, Jeff A.
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Connelly, Douglas P.
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Pearce, David
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Linse, Katrin
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Zwirglmaier, Katrin, Reid, William D. K., Heywood, Jane, Sweeting, Christopher J., Wigham, Benjamin D., Polunin, Nicholas V. C., Hawkes, Jeff A., Connelly, Douglas P., Pearce, David and Linse, Katrin (2015) Linking regional variation of epibiotic bacterial diversity and trophic ecology in a new species of Kiwaidae (Decapoda, Anomura) from East Scotia Ridge (Antarctica) hydrothermal vents. MicrobiologyOpen, 4 (1), 136-150. (doi:10.1002/mbo3.227).

Record type: Article

Abstract

We analyzed the diversity of bacterial epibionts and trophic ecology of a new species of Kiwa yeti crab discovered at two hydrothermal vent fields (E2 and E9) on the East Scotia Ridge (ESR) in the Southern Ocean using a combination of 454 pyrosequencing, Sanger sequencing, and stable isotope analysis. The Kiwa epibiont communities were dominated by Epsilon- and Gammaproteobacteria. About 454 sequencing of the epibionts on 15 individual Kiwa specimen revealed large regional differences between the two hydrothermal vent fields: at E2, the bacterial community on the Kiwa ventral setae was dominated (up to 75%) by Gammaproteobacteria, whereas at E9 Epsilonproteobacteria dominated (up to 98%). Carbon stable isotope analysis of both Kiwa and the bacterial epibionts also showed distinct differences between E2 and E9 in mean and variability. Both stable isotope and sequence data suggest a dominance of different carbon fixation pathways of the epibiont communities at the two vent fields. At E2, epibionts were putatively fixing carbon via the Calvin-Benson-Bassham and reverse tricarboxylic acid cycle, while at E9 the reverse tricarboxylic acid cycle dominated. Co-varying epibiont diversity and isotope values at E2 and E9 also present further support for the hypothesis that epibionts serve as a food source for Kiwa.

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e-pub ahead of print date: 16 December 2014
Published date: February 2015
Keywords: East Scotia Ridge, epibionts, hydrothermal vent, Kiwa sp., microbial diversity, stable isotopes
Organisations: Ocean and Earth Science, Marine Geoscience

Identifiers

Local EPrints ID: 372822
URI: http://eprints.soton.ac.uk/id/eprint/372822
ISSN: 2045-8827
PURE UUID: 7727bb79-3b17-489b-997e-404c23bdae74

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Date deposited: 18 Dec 2014 10:43
Last modified: 08 Jan 2022 00:35

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Contributors

Author: Katrin Zwirglmaier
Author: William D. K. Reid
Author: Jane Heywood
Author: Christopher J. Sweeting
Author: Benjamin D. Wigham
Author: Nicholas V. C. Polunin
Author: Jeff A. Hawkes
Author: Douglas P. Connelly
Author: David Pearce
Author: Katrin Linse

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