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Temperature-dependent resetting of the molecular circadian oscillator in Drosophila

Temperature-dependent resetting of the molecular circadian oscillator in Drosophila
Temperature-dependent resetting of the molecular circadian oscillator in Drosophila
Circadian clocks responsible for daily time keeping in a wide range of organisms synchronize to daily temperature cycles via pathways that remain poorly understood. To address this problem from the perspective of the molecular oscillator, we monitored temperature-dependent resetting of four of its core components in the fruitfly Drosophila melanogaster: the transcripts and proteins for the clock genes period (per) and timeless (tim). The molecular circadian cycle in adult heads exhibited parallel responses to temperature-mediated resetting at the levels of per transcript, tim transcript and TIM protein. Early phase adjustment specific to per transcript rhythms was explained by clock-independent temperature-driven transcription of per. The cold-induced expression of Drosophila per contrasts with the previously reported heat-induced regulation of mammalian Period 2. An altered and more readily re-entrainable temperature-synchronized circadian oscillator that featured temperature-driven per transcript rhythms and phase-shifted TIM and PER protein rhythms was found for flies of the 'Tim 4' genotype, which lacked daily tim transcript oscillations but maintained post-transcriptional temperature entrainment of tim expression. The accelerated molecular and behavioural temperature entrainment observed for Tim 4 flies indicates that clock-controlled tim expression constrains the rate of temperature cycle-mediated circadian resetting
1471-2954
Goda, Tadahiro
e630d6d8-4e4b-48e5-85ee-5a4f0e56c0d4
Sharp, Brandi
653ea954-b5aa-4313-a7df-34efa1a59cf3
Wijnen, Herman
67e9bc5d-de6e-44ec-b4c2-50b67c5bc79d
Goda, Tadahiro
e630d6d8-4e4b-48e5-85ee-5a4f0e56c0d4
Sharp, Brandi
653ea954-b5aa-4313-a7df-34efa1a59cf3
Wijnen, Herman
67e9bc5d-de6e-44ec-b4c2-50b67c5bc79d

Goda, Tadahiro, Sharp, Brandi and Wijnen, Herman (2014) Temperature-dependent resetting of the molecular circadian oscillator in Drosophila. Proceedings of the Royal Society B, 281 (1793). (doi:10.1098/rspb.2014.1714). (PMID:25165772)

Record type: Article

Abstract

Circadian clocks responsible for daily time keeping in a wide range of organisms synchronize to daily temperature cycles via pathways that remain poorly understood. To address this problem from the perspective of the molecular oscillator, we monitored temperature-dependent resetting of four of its core components in the fruitfly Drosophila melanogaster: the transcripts and proteins for the clock genes period (per) and timeless (tim). The molecular circadian cycle in adult heads exhibited parallel responses to temperature-mediated resetting at the levels of per transcript, tim transcript and TIM protein. Early phase adjustment specific to per transcript rhythms was explained by clock-independent temperature-driven transcription of per. The cold-induced expression of Drosophila per contrasts with the previously reported heat-induced regulation of mammalian Period 2. An altered and more readily re-entrainable temperature-synchronized circadian oscillator that featured temperature-driven per transcript rhythms and phase-shifted TIM and PER protein rhythms was found for flies of the 'Tim 4' genotype, which lacked daily tim transcript oscillations but maintained post-transcriptional temperature entrainment of tim expression. The accelerated molecular and behavioural temperature entrainment observed for Tim 4 flies indicates that clock-controlled tim expression constrains the rate of temperature cycle-mediated circadian resetting

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More information

Accepted/In Press date: 1 August 2014
e-pub ahead of print date: 27 August 2014
Published date: 22 October 2014
Organisations: Centre for Biological Sciences

Identifiers

Local EPrints ID: 372858
URI: http://eprints.soton.ac.uk/id/eprint/372858
ISSN: 1471-2954
PURE UUID: b2953990-74cb-4c91-be1e-1f542f742e8f
ORCID for Herman Wijnen: ORCID iD orcid.org/0000-0002-8710-5176

Catalogue record

Date deposited: 22 Dec 2014 14:15
Last modified: 12 Nov 2019 01:38

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