Screening ion-channel ligand interactions with passive pumping in a microfluidic bilayer lipid membrane (BLM) chip
Screening ion-channel ligand interactions with passive pumping in a microfluidic bilayer lipid membrane (BLM) chip
We describe a scalable artificial bilayer lipid membrane (BLM) platform for rapid electrophysiological screening of ion channels and transporters. A passive pumping method is used to flow microliter volumes of ligand solution across a suspended bilayer within a microfluidic chip. Bilayers are stable at flow rates up to ?0.5 µL/min. Phospholipid bilayers are formed across a photolithographically defined aperture made in a dry film resist within the microfluidic chip. Bilayers are stable for many days and the low shunt capacitance of the thin film support gives low-noise high-quality single ion channel recording. Dose-dependent transient blocking of ?-hemolysin with ?-cyclodextrin (?-CD) and polyethylene glycol (PEG) is demonstrated and dose-dependent blocking studies of the KcsA potassium channel with tetraethylammonium (TEA) show the potential for determining IC50 values. The assays are fast (30 minutes for a complete IC50 curve) and simple and require very small amounts of compounds (100 ?g in 15 ?L). The technology can be scaled so that multiple bilayers can be addressed, providing a screening platform for ion channels, transporters and nanopores.
14103
Saha, Shimul C.
e2c8a09a-21af-4a31-bd25-26945094e03a
Powl, Andrew M.
79b77ec3-767b-42ad-991c-d843e29af8e3
Wallace, B. A.
0de71d92-1b00-4da1-8567-10160db20fe5
de Planque, Maurits R.R.
a1d33d13-f516-44fb-8d2c-c51d18bc21ba
Morgan, Hywel
de00d59f-a5a2-48c4-a99a-1d5dd7854174
9 January 2015
Saha, Shimul C.
e2c8a09a-21af-4a31-bd25-26945094e03a
Powl, Andrew M.
79b77ec3-767b-42ad-991c-d843e29af8e3
Wallace, B. A.
0de71d92-1b00-4da1-8567-10160db20fe5
de Planque, Maurits R.R.
a1d33d13-f516-44fb-8d2c-c51d18bc21ba
Morgan, Hywel
de00d59f-a5a2-48c4-a99a-1d5dd7854174
Saha, Shimul C., Powl, Andrew M., Wallace, B. A., de Planque, Maurits R.R. and Morgan, Hywel
(2015)
Screening ion-channel ligand interactions with passive pumping in a microfluidic bilayer lipid membrane (BLM) chip.
Biomicrofluidics, 9, .
(doi:10.1063/1.4905313).
Abstract
We describe a scalable artificial bilayer lipid membrane (BLM) platform for rapid electrophysiological screening of ion channels and transporters. A passive pumping method is used to flow microliter volumes of ligand solution across a suspended bilayer within a microfluidic chip. Bilayers are stable at flow rates up to ?0.5 µL/min. Phospholipid bilayers are formed across a photolithographically defined aperture made in a dry film resist within the microfluidic chip. Bilayers are stable for many days and the low shunt capacitance of the thin film support gives low-noise high-quality single ion channel recording. Dose-dependent transient blocking of ?-hemolysin with ?-cyclodextrin (?-CD) and polyethylene glycol (PEG) is demonstrated and dose-dependent blocking studies of the KcsA potassium channel with tetraethylammonium (TEA) show the potential for determining IC50 values. The assays are fast (30 minutes for a complete IC50 curve) and simple and require very small amounts of compounds (100 ?g in 15 ?L). The technology can be scaled so that multiple bilayers can be addressed, providing a screening platform for ion channels, transporters and nanopores.
Text
Saha2015.pdf
- Accepted Manuscript
More information
Published date: 9 January 2015
Organisations:
Nanoelectronics and Nanotechnology
Identifiers
Local EPrints ID: 372860
URI: http://eprints.soton.ac.uk/id/eprint/372860
ISSN: 1932-1058
PURE UUID: 5f1b9e76-c4bc-432f-baf5-ceb6f17adfb3
Catalogue record
Date deposited: 19 Dec 2014 14:56
Last modified: 15 Mar 2024 03:18
Export record
Altmetrics
Contributors
Author:
Shimul C. Saha
Author:
Andrew M. Powl
Author:
B. A. Wallace
Author:
Maurits R.R. de Planque
Author:
Hywel Morgan
Download statistics
Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.
View more statistics