Screening ion-channel ligand interactions with passive pumping in a microfluidic bilayer lipid membrane (BLM) chip


Saha, Shimul C., Powl, Andrew M., Wallace, B. A., de Planque, Maurits R.R. and Morgan, Hywel (2015) Screening ion-channel ligand interactions with passive pumping in a microfluidic bilayer lipid membrane (BLM) chip Biomicrofluidics, 9, p. 14103. (doi:10.1063/1.4905313).

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Description/Abstract

We describe a scalable artificial bilayer lipid membrane (BLM) platform for rapid electrophysiological screening of ion channels and transporters. A passive pumping method is used to flow microliter volumes of ligand solution across a suspended bilayer within a microfluidic chip. Bilayers are stable at flow rates up to ?0.5 µL/min. Phospholipid bilayers are formed across a photolithographically defined aperture made in a dry film resist within the microfluidic chip. Bilayers are stable for many days and the low shunt capacitance of the thin film support gives low-noise high-quality single ion channel recording. Dose-dependent transient blocking of ?-hemolysin with ?-cyclodextrin (?-CD) and polyethylene glycol (PEG) is demonstrated and dose-dependent blocking studies of the KcsA potassium channel with tetraethylammonium (TEA) show the potential for determining IC50 values. The assays are fast (30 minutes for a complete IC50 curve) and simple and require very small amounts of compounds (100 ?g in 15 ?L). The technology can be scaled so that multiple bilayers can be addressed, providing a screening platform for ion channels, transporters and nanopores.

Item Type: Article
Digital Object Identifier (DOI): doi:10.1063/1.4905313
ISSNs: 1932-1058 (print)
Organisations: Nanoelectronics and Nanotechnology
ePrint ID: 372860
Date :
Date Event
9 January 2015Published
Date Deposited: 19 Dec 2014 14:56
Last Modified: 17 Apr 2017 06:54
Further Information:Google Scholar
URI: http://eprints.soton.ac.uk/id/eprint/372860

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