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Functional analyses of differentially expressed isoforms of the Arabidopsis inositol phosphorylceramide synthase

Functional analyses of differentially expressed isoforms of the Arabidopsis inositol phosphorylceramide synthase
Functional analyses of differentially expressed isoforms of the Arabidopsis inositol phosphorylceramide synthase
Sphingolipids are key components of eukaryotic plasma membranes that are involved in many functions, including the formation signal transduction complexes. In addition, these lipid species and their catabolites function as secondary signalling molecules in, amongst other processes, apoptosis. The biosynthetic pathway for the formation of sphingolipid is largely conserved. However, unlike mammalian cells, fungi, protozoa and plants synthesize inositol phosphorylceramide (IPC) as their primary phosphosphingolipid. This key step involves the transfer of the phosphorylinositol group from phosphatidylinositol (PI) to phytoceramide, a process catalysed by IPC synthase in plants and fungi. This enzyme activity is at least partly encoded by the AUR1 gene in the fungi, and recently the distantly related functional orthologue of this gene has been identified in the model plant Arabidopsis. Here we functionally analysed all three predicted Arabidopsis IPC synthases, confirming them as aureobasidin A resistant AUR1p orthologues. Expression profiling revealed that the genes encoding these orthologues are differentially expressed in various tissue types isolated from Arabidopsis.
Arabidopsis, AUR1, expression, inositol phosphorylceramide IPC synthase, sphingolipids
0167-4412
399-407
Denny, P.W.
41626b4a-6f75-460f-a2a0-2018a93ef8aa
Mina, J.G.
a239b57a-b3cb-41fe-965d-8211ca14a10e
Okada, Y.
9bb54e95-06af-432b-a68b-af02322edc46
Wansadhipathi-Kannangara, N.K.
a2c031cf-a6b1-4ccf-af88-c41cedda3c00
Pratt, S.
199787ce-3331-40a9-8cc4-1da3300f4511
Shams-Eldin, H.
25b60f10-46be-4ad4-ae81-744330841b23
Schwarz, R.T.
c0324543-722f-4d27-bba5-56fb5c5759a8
Steel, P.G.
621274e7-be77-4fdb-8773-1c154188b3ed
Fawcett, T.
33a48c55-43ef-4019-9200-ac95287bf230
Denny, P.W.
41626b4a-6f75-460f-a2a0-2018a93ef8aa
Mina, J.G.
a239b57a-b3cb-41fe-965d-8211ca14a10e
Okada, Y.
9bb54e95-06af-432b-a68b-af02322edc46
Wansadhipathi-Kannangara, N.K.
a2c031cf-a6b1-4ccf-af88-c41cedda3c00
Pratt, S.
199787ce-3331-40a9-8cc4-1da3300f4511
Shams-Eldin, H.
25b60f10-46be-4ad4-ae81-744330841b23
Schwarz, R.T.
c0324543-722f-4d27-bba5-56fb5c5759a8
Steel, P.G.
621274e7-be77-4fdb-8773-1c154188b3ed
Fawcett, T.
33a48c55-43ef-4019-9200-ac95287bf230

Denny, P.W., Mina, J.G., Okada, Y., Wansadhipathi-Kannangara, N.K., Pratt, S., Shams-Eldin, H., Schwarz, R.T., Steel, P.G. and Fawcett, T. (2010) Functional analyses of differentially expressed isoforms of the Arabidopsis inositol phosphorylceramide synthase. Plant Molecular Biology, 73 (4-5), 399-407. (doi:10.1007/s11103-010-9626-3). (PMID:20309609)

Record type: Article

Abstract

Sphingolipids are key components of eukaryotic plasma membranes that are involved in many functions, including the formation signal transduction complexes. In addition, these lipid species and their catabolites function as secondary signalling molecules in, amongst other processes, apoptosis. The biosynthetic pathway for the formation of sphingolipid is largely conserved. However, unlike mammalian cells, fungi, protozoa and plants synthesize inositol phosphorylceramide (IPC) as their primary phosphosphingolipid. This key step involves the transfer of the phosphorylinositol group from phosphatidylinositol (PI) to phytoceramide, a process catalysed by IPC synthase in plants and fungi. This enzyme activity is at least partly encoded by the AUR1 gene in the fungi, and recently the distantly related functional orthologue of this gene has been identified in the model plant Arabidopsis. Here we functionally analysed all three predicted Arabidopsis IPC synthases, confirming them as aureobasidin A resistant AUR1p orthologues. Expression profiling revealed that the genes encoding these orthologues are differentially expressed in various tissue types isolated from Arabidopsis.

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Published date: 15 July 2010
Keywords: Arabidopsis, AUR1, expression, inositol phosphorylceramide IPC synthase, sphingolipids
Organisations: Faculty of Medicine

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Local EPrints ID: 373081
URI: http://eprints.soton.ac.uk/id/eprint/373081
ISSN: 0167-4412
PURE UUID: 8a264d05-dd5c-45d9-b5f2-f00e91593bef

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Date deposited: 06 Jan 2015 16:20
Last modified: 15 Jul 2019 21:34

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Contributors

Author: P.W. Denny
Author: J.G. Mina
Author: Y. Okada
Author: N.K. Wansadhipathi-Kannangara
Author: S. Pratt
Author: H. Shams-Eldin
Author: R.T. Schwarz
Author: P.G. Steel
Author: T. Fawcett

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