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Predicting leaf waxn-alkane2H/1H ratios: controlled water source and humidity experiments with hydroponically grown trees confirm predictions of Craig-Gordon model

Predicting leaf waxn-alkane2H/1H ratios: controlled water source and humidity experiments with hydroponically grown trees confirm predictions of Craig-Gordon model
Predicting leaf waxn-alkane2H/1H ratios: controlled water source and humidity experiments with hydroponically grown trees confirm predictions of Craig-Gordon model
The extent to which both water source and atmospheric humidity affect ?2H values of terrestrial plant leaf waxes will affect the interpretations of ?2H variation of leaf waxes as a proxy for hydrological conditions. To elucidate the effects of these parameters, we conducted a long-term experiment in which we grew two tree species, Populus fremontii and Betula occidentalis, hydroponically under combinations of six isotopically distinct waters and two different atmospheric humidities. We observed that leaf n-alkane ?2H values of both species were linearly related to source water ?2H values, but with slope differences associated with differing humidities. When a modified version of the Craig–Gordon model incorporating plant factors was used to predict the ?2H values of leaf water, all modelled leaf water values fit the same linear relationship with n-alkane ?2H values. These observations suggested a relatively constant biosynthetic fractionation factor between leaf water and n-alkanes. However, our calculations indicated a small difference in the biosynthetic fractionation factor between the two species, consistent with small differences calculated for species in other studies. At present, it remains unclear if these apparent interspecies differences in biosynthetic fractionation reflect species-specific biochemistry or a common biosynthetic fractionation factor with insufficient model parameterization.
compound-specific isotope analysis, hydrogen isotopes, stable isotopes
0140-7791
1035–1047
Tipple, Brett J.
04f91c16-e530-4cf9-9515-a66b1113a610
Berke, Melissa A.
c7b3130d-6e7a-4f94-ba6e-b58f5d45c956
Hambach, Bastian
d144b919-bc71-4793-89ee-7fdfdb7762bb
Roden, John S.
bbfcdf7c-8621-43e3-b717-adb4bec1ffdd
Ehleringer, James R.
17f1234e-0510-4a58-b4a6-b496e0cd917d
Tipple, Brett J.
04f91c16-e530-4cf9-9515-a66b1113a610
Berke, Melissa A.
c7b3130d-6e7a-4f94-ba6e-b58f5d45c956
Hambach, Bastian
d144b919-bc71-4793-89ee-7fdfdb7762bb
Roden, John S.
bbfcdf7c-8621-43e3-b717-adb4bec1ffdd
Ehleringer, James R.
17f1234e-0510-4a58-b4a6-b496e0cd917d

Tipple, Brett J., Berke, Melissa A., Hambach, Bastian, Roden, John S. and Ehleringer, James R. (2015) Predicting leaf waxn-alkane2H/1H ratios: controlled water source and humidity experiments with hydroponically grown trees confirm predictions of Craig-Gordon model. Plant, Cell & Environment, 38 (6), 1035–1047. (doi:10.1111/pce.12457).

Record type: Article

Abstract

The extent to which both water source and atmospheric humidity affect ?2H values of terrestrial plant leaf waxes will affect the interpretations of ?2H variation of leaf waxes as a proxy for hydrological conditions. To elucidate the effects of these parameters, we conducted a long-term experiment in which we grew two tree species, Populus fremontii and Betula occidentalis, hydroponically under combinations of six isotopically distinct waters and two different atmospheric humidities. We observed that leaf n-alkane ?2H values of both species were linearly related to source water ?2H values, but with slope differences associated with differing humidities. When a modified version of the Craig–Gordon model incorporating plant factors was used to predict the ?2H values of leaf water, all modelled leaf water values fit the same linear relationship with n-alkane ?2H values. These observations suggested a relatively constant biosynthetic fractionation factor between leaf water and n-alkanes. However, our calculations indicated a small difference in the biosynthetic fractionation factor between the two species, consistent with small differences calculated for species in other studies. At present, it remains unclear if these apparent interspecies differences in biosynthetic fractionation reflect species-specific biochemistry or a common biosynthetic fractionation factor with insufficient model parameterization.

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More information

Accepted/In Press date: 23 September 2014
e-pub ahead of print date: 2014
Published date: 1 June 2015
Keywords: compound-specific isotope analysis, hydrogen isotopes, stable isotopes
Organisations: Ocean and Earth Science, Paleooceanography & Palaeoclimate

Identifiers

Local EPrints ID: 373724
URI: http://eprints.soton.ac.uk/id/eprint/373724
ISSN: 0140-7791
PURE UUID: b223d54d-88e5-44d1-a2ed-4a3bbe3affa0
ORCID for Bastian Hambach: ORCID iD orcid.org/0000-0003-4546-5672

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Date deposited: 04 Feb 2015 11:24
Last modified: 09 Jan 2022 03:48

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Contributors

Author: Brett J. Tipple
Author: Melissa A. Berke
Author: Bastian Hambach ORCID iD
Author: John S. Roden
Author: James R. Ehleringer

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