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Characterizing microbial community and geochemical dynamics at hydrothermal vents using osmotically-driven continuous fluid samplers

Characterizing microbial community and geochemical dynamics at hydrothermal vents using osmotically-driven continuous fluid samplers
Characterizing microbial community and geochemical dynamics at hydrothermal vents using osmotically-driven continuous fluid samplers
Microbes play a key role in mediating aquatic biogeochemical cycles. However, our understanding of the relationships between microbial phylogenetic/physiological diversity and habitat physicochemical characteristics is restrained by our limited capacity to concurrently collect microbial and geochemical samples at appropriate spatial and temporal scales. Accordingly, we have developed a low-cost, continuous fluid sampling system (the Biological OsmoSampling System, or BOSS) to address this limitation. The BOSS does not use electricity, can be deployed in harsh/remote environments, and collects/preserves samples with daily resolution for >1 year. Here, we present data on the efficacy of DNA and protein preservation during a 1.5 year laboratory study as well as the results of two field deployments at deep-sea hydrothermal vents, wherein we examined changes in microbial diversity, protein expression, and geochemistry over time. Our data reveal marked changes in microbial composition co-occurring with changes in hydrothermal fluid composition as well as the temporal dynamics of an enigmatic sulfide-oxidizing symbiont in its free-living state. We also present the first data on in situ protein preservation and expression dynamics highlighting the BOSS’s potential utility in meta-proteomic studies. These data illustrate the value of using BOSS to study relationships among microbial and geochemical phenomena and environmental conditions.
0013-936X
4399-4407
Robidart, Julie
a9b8d49c-c1e3-4a3b-a53c-685a0f2c7f93
Callister, Stephen J.
13132fc6-d1b6-4557-853d-ff5863b106c0
Song, Pengfei
3782998a-89e4-4b87-a879-26ebdbd98590
Nicora, Carrie D.
418b9a84-cb34-4ed2-8f41-acead95d8e95
Wheat, Charles G.
57649c55-907a-4fd6-9e71-a1bf7a978980
Girguis, Peter R.
fdaadd84-238b-4d37-b030-b5c4749269f7
Robidart, Julie
a9b8d49c-c1e3-4a3b-a53c-685a0f2c7f93
Callister, Stephen J.
13132fc6-d1b6-4557-853d-ff5863b106c0
Song, Pengfei
3782998a-89e4-4b87-a879-26ebdbd98590
Nicora, Carrie D.
418b9a84-cb34-4ed2-8f41-acead95d8e95
Wheat, Charles G.
57649c55-907a-4fd6-9e71-a1bf7a978980
Girguis, Peter R.
fdaadd84-238b-4d37-b030-b5c4749269f7

Robidart, Julie, Callister, Stephen J., Song, Pengfei, Nicora, Carrie D., Wheat, Charles G. and Girguis, Peter R. (2013) Characterizing microbial community and geochemical dynamics at hydrothermal vents using osmotically-driven continuous fluid samplers. Environmental Science & Technology, 47 (9), 4399-4407. (doi:10.1021/es3037302).

Record type: Article

Abstract

Microbes play a key role in mediating aquatic biogeochemical cycles. However, our understanding of the relationships between microbial phylogenetic/physiological diversity and habitat physicochemical characteristics is restrained by our limited capacity to concurrently collect microbial and geochemical samples at appropriate spatial and temporal scales. Accordingly, we have developed a low-cost, continuous fluid sampling system (the Biological OsmoSampling System, or BOSS) to address this limitation. The BOSS does not use electricity, can be deployed in harsh/remote environments, and collects/preserves samples with daily resolution for >1 year. Here, we present data on the efficacy of DNA and protein preservation during a 1.5 year laboratory study as well as the results of two field deployments at deep-sea hydrothermal vents, wherein we examined changes in microbial diversity, protein expression, and geochemistry over time. Our data reveal marked changes in microbial composition co-occurring with changes in hydrothermal fluid composition as well as the temporal dynamics of an enigmatic sulfide-oxidizing symbiont in its free-living state. We also present the first data on in situ protein preservation and expression dynamics highlighting the BOSS’s potential utility in meta-proteomic studies. These data illustrate the value of using BOSS to study relationships among microbial and geochemical phenomena and environmental conditions.

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More information

Published date: March 2013
Organisations: Ocean Technology and Engineering

Identifiers

Local EPrints ID: 374044
URI: http://eprints.soton.ac.uk/id/eprint/374044
ISSN: 0013-936X
PURE UUID: 57974949-2540-4b5f-8ccb-edf21a63071d

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Date deposited: 02 Feb 2015 15:21
Last modified: 14 Mar 2024 19:01

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Contributors

Author: Julie Robidart
Author: Stephen J. Callister
Author: Pengfei Song
Author: Carrie D. Nicora
Author: Charles G. Wheat
Author: Peter R. Girguis

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