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Metabolic versatility of the Riftia pachyptila endosymbiont revealed through metagenomics

Metabolic versatility of the Riftia pachyptila endosymbiont revealed through metagenomics
Metabolic versatility of the Riftia pachyptila endosymbiont revealed through metagenomics
The facultative symbiont of Riftia pachyptila, named here Candidatus Endoriftia persephone, has evaded culture to date, but much has been learned regarding this symbiosis over the past three decades since its discovery. The symbiont population metagenome was sequenced in order to gain insight into its physiology. The population genome indicates that the symbionts use a partial Calvin–Benson Cycle for carbon fixation and the reverse TCA cycle (an alternative pathway for carbon fixation) that contains an unusual ATP citrate lyase. The presence of all genes necessary for heterotrophic metabolism, a phosphotransferase system, and dicarboxylate and ABC transporters indicate that the symbiont can live mixotrophically. The metagenome has a large suite of signal transduction, defence (both biological and environmental) and chemotaxis mechanisms. The physiology of Candidatus Endoriftia persephone is explored with respect to functionality while associated with a eukaryotic host, versus free-living in the hydrothermal environment.
1462-2920
727-737
Robidart, Julie C.
1d72ac92-7dca-49a0-9f9e-26798387e66b
Bench, Shellie R.
3af1690f-2f4e-49c0-a747-99a711e3b6e1
Feldman, Robert A.
74ac28bd-6fa6-4066-b0cc-1786992ace88
Novoradovsky, Alexey
daafb9cd-bc02-4fb2-921d-b14affea11c7
Podell, Sheila B.
51a1a29d-4e3a-4a26-adfa-9db83b3b79a4
Gaasterland, Terry
d411455e-ccaa-4204-a10a-af73d31885dc
Allen, Eric E.
15858222-857d-4031-b8b6-4f1dcead3fe7
Felbeck, Horst
2fd18f7c-ef35-4faa-82db-c3d2665e0523
Robidart, Julie C.
1d72ac92-7dca-49a0-9f9e-26798387e66b
Bench, Shellie R.
3af1690f-2f4e-49c0-a747-99a711e3b6e1
Feldman, Robert A.
74ac28bd-6fa6-4066-b0cc-1786992ace88
Novoradovsky, Alexey
daafb9cd-bc02-4fb2-921d-b14affea11c7
Podell, Sheila B.
51a1a29d-4e3a-4a26-adfa-9db83b3b79a4
Gaasterland, Terry
d411455e-ccaa-4204-a10a-af73d31885dc
Allen, Eric E.
15858222-857d-4031-b8b6-4f1dcead3fe7
Felbeck, Horst
2fd18f7c-ef35-4faa-82db-c3d2665e0523

Robidart, Julie C., Bench, Shellie R., Feldman, Robert A., Novoradovsky, Alexey, Podell, Sheila B., Gaasterland, Terry, Allen, Eric E. and Felbeck, Horst (2008) Metabolic versatility of the Riftia pachyptila endosymbiont revealed through metagenomics. Environmental Microbiology, 10 (3), 727-737. (doi:10.1111/j.1462-2920.2007.01496.x).

Record type: Article

Abstract

The facultative symbiont of Riftia pachyptila, named here Candidatus Endoriftia persephone, has evaded culture to date, but much has been learned regarding this symbiosis over the past three decades since its discovery. The symbiont population metagenome was sequenced in order to gain insight into its physiology. The population genome indicates that the symbionts use a partial Calvin–Benson Cycle for carbon fixation and the reverse TCA cycle (an alternative pathway for carbon fixation) that contains an unusual ATP citrate lyase. The presence of all genes necessary for heterotrophic metabolism, a phosphotransferase system, and dicarboxylate and ABC transporters indicate that the symbiont can live mixotrophically. The metagenome has a large suite of signal transduction, defence (both biological and environmental) and chemotaxis mechanisms. The physiology of Candidatus Endoriftia persephone is explored with respect to functionality while associated with a eukaryotic host, versus free-living in the hydrothermal environment.

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Published date: March 2008
Organisations: Ocean Technology and Engineering

Identifiers

Local EPrints ID: 374101
URI: http://eprints.soton.ac.uk/id/eprint/374101
ISSN: 1462-2920
PURE UUID: 23ed3320-1309-49c3-97e0-63583a4c4ced

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Date deposited: 03 Feb 2015 14:03
Last modified: 25 Nov 2021 17:09

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Contributors

Author: Julie C. Robidart
Author: Shellie R. Bench
Author: Robert A. Feldman
Author: Alexey Novoradovsky
Author: Sheila B. Podell
Author: Terry Gaasterland
Author: Eric E. Allen
Author: Horst Felbeck

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