IL-1?-dependent activation of NF-?B mediates PGE2 release via the expression of cyclooxygenase-2 and microsomal prostaglandin E synthase

Catley, Matthew C., Chivers, Joanna E., Cambridge, Lisa M., Holden, Neil, Slater, Donna M., Staples, Karl J., Bergmann, Martin W., Loser, Peter, Barnes, Peter J. and Newton, Robert (2003) IL-1?-dependent activation of NF-?B mediates PGE2 release via the expression of cyclooxygenase-2 and microsomal prostaglandin E synthase. FEBS Letters, 547 (1-3), 75-79. (doi:10.1016/S0014-5793(03)00672-0).

Abstract

Prostaglandin (PG) E2 release is induced in pulmonary A549 cells by the NF-?B-activating stimuli interleukin-1? (IL-1?) and phorbol 12-myristate 13-acetate (PMA). Adenoviral over-expression of I?B??N, a dominant NF-?B inhibitor, prevents NF-?B-dependent transcription and was used to qualify the role of NF-?B in the release of PGE2. I?B??N repressed IL-1?-induced, but not PMA-induced, cycloxygenase-2 (COX-2) and microsomal prostaglandin E synthase (mPGES) expression. These data conclusively demonstrate a substantial role for NF-?B in the co-ordinate induction of COX-2, mPGES and in the corresponding release of PGE2 by IL-1?. However, other pathways are primarily responsible for PGE2 release induced by PMA

This record has no associated files available for download.

Contributors

Author: Karl J. Staples

Download statistics