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IL-1?-dependent activation of NF-?B mediates PGE2 release via the expression of cyclooxygenase-2 and microsomal prostaglandin E synthase

IL-1?-dependent activation of NF-?B mediates PGE2 release via the expression of cyclooxygenase-2 and microsomal prostaglandin E synthase
IL-1?-dependent activation of NF-?B mediates PGE2 release via the expression of cyclooxygenase-2 and microsomal prostaglandin E synthase
Prostaglandin (PG) E2 release is induced in pulmonary A549 cells by the NF-?B-activating stimuli interleukin-1? (IL-1?) and phorbol 12-myristate 13-acetate (PMA). Adenoviral over-expression of I?B??N, a dominant NF-?B inhibitor, prevents NF-?B-dependent transcription and was used to qualify the role of NF-?B in the release of PGE2. I?B??N repressed IL-1?-induced, but not PMA-induced, cycloxygenase-2 (COX-2) and microsomal prostaglandin E synthase (mPGES) expression. These data conclusively demonstrate a substantial role for NF-?B in the co-ordinate induction of COX-2, mPGES and in the corresponding release of PGE2 by IL-1?. However, other pathways are primarily responsible for PGE2 release induced by PMA
0014-5793
75-79
Catley, Matthew C.
8b86591b-8338-4241-8999-5ea259af7ca4
Chivers, Joanna E.
4f4587b1-71ab-4164-8c8b-0ed50671a1e0
Cambridge, Lisa M.
95d678f3-91e6-4720-8d0a-d3572d05dd6a
Holden, Neil
66ece70c-22dc-462f-9668-e4871475584d
Slater, Donna M.
12698b95-4932-4f3e-9ace-abac4f334fa9
Staples, Karl J.
e0e9d80f-0aed-435f-bd75-0c8818491fee
Bergmann, Martin W.
569898b9-1e22-497d-964b-85bb6741f48c
Loser, Peter
2fd6d4f6-ede1-47ba-9f64-e05793e26299
Barnes, Peter J.
6cbf850c-3499-4e23-983c-4442643c0004
Newton, Robert
4a2c2a99-b7ad-4373-8840-96fbb5b5313e
Catley, Matthew C.
8b86591b-8338-4241-8999-5ea259af7ca4
Chivers, Joanna E.
4f4587b1-71ab-4164-8c8b-0ed50671a1e0
Cambridge, Lisa M.
95d678f3-91e6-4720-8d0a-d3572d05dd6a
Holden, Neil
66ece70c-22dc-462f-9668-e4871475584d
Slater, Donna M.
12698b95-4932-4f3e-9ace-abac4f334fa9
Staples, Karl J.
e0e9d80f-0aed-435f-bd75-0c8818491fee
Bergmann, Martin W.
569898b9-1e22-497d-964b-85bb6741f48c
Loser, Peter
2fd6d4f6-ede1-47ba-9f64-e05793e26299
Barnes, Peter J.
6cbf850c-3499-4e23-983c-4442643c0004
Newton, Robert
4a2c2a99-b7ad-4373-8840-96fbb5b5313e

Catley, Matthew C., Chivers, Joanna E., Cambridge, Lisa M., Holden, Neil, Slater, Donna M., Staples, Karl J., Bergmann, Martin W., Loser, Peter, Barnes, Peter J. and Newton, Robert (2003) IL-1?-dependent activation of NF-?B mediates PGE2 release via the expression of cyclooxygenase-2 and microsomal prostaglandin E synthase. FEBS Letters, 547 (1-3), 75-79. (doi:10.1016/S0014-5793(03)00672-0).

Record type: Article

Abstract

Prostaglandin (PG) E2 release is induced in pulmonary A549 cells by the NF-?B-activating stimuli interleukin-1? (IL-1?) and phorbol 12-myristate 13-acetate (PMA). Adenoviral over-expression of I?B??N, a dominant NF-?B inhibitor, prevents NF-?B-dependent transcription and was used to qualify the role of NF-?B in the release of PGE2. I?B??N repressed IL-1?-induced, but not PMA-induced, cycloxygenase-2 (COX-2) and microsomal prostaglandin E synthase (mPGES) expression. These data conclusively demonstrate a substantial role for NF-?B in the co-ordinate induction of COX-2, mPGES and in the corresponding release of PGE2 by IL-1?. However, other pathways are primarily responsible for PGE2 release induced by PMA

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Published date: July 2003
Organisations: Faculty of Medicine

Identifiers

Local EPrints ID: 374409
URI: https://eprints.soton.ac.uk/id/eprint/374409
ISSN: 0014-5793
PURE UUID: 56859dbf-9ebc-469a-9d13-00df0c382f65
ORCID for Karl J. Staples: ORCID iD orcid.org/0000-0003-3844-6457

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Date deposited: 16 Feb 2015 13:16
Last modified: 20 Jul 2019 00:53

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Contributors

Author: Matthew C. Catley
Author: Joanna E. Chivers
Author: Lisa M. Cambridge
Author: Neil Holden
Author: Donna M. Slater
Author: Karl J. Staples ORCID iD
Author: Martin W. Bergmann
Author: Peter Loser
Author: Peter J. Barnes
Author: Robert Newton

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