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Evidence for systemic rather than pulmonary effects of interleukin-5 administration in asthma

Evidence for systemic rather than pulmonary effects of interleukin-5 administration in asthma
Evidence for systemic rather than pulmonary effects of interleukin-5 administration in asthma
Background: interleukin 5 (IL-5) has an important role in mobilisation of eosinophils from the bone marrow and in their subsequent terminal differentiation. A study was undertaken to determine whether inhaled and intravenous IL-5 could induce pulmonary eosinophilia and bronchial hyperresponsiveness (BHR) independently of these effects.

Methods: nine mild asthmatics received inhaled (15??g) or intravenous (2??g) IL-5 or placebo in random order in a double blind, crossover study. Blood samples were taken before and at 0.5, 1, 2, 3, 4, 5, 24, and 72 hours following IL-5 or placebo, and bronchial responsiveness (PC20 methacholine) and eosinophil counts in induced sputum were determined.

Results: serum IL-5 levels were markedly increased 30 minutes after intravenous IL-5 (p=0.002), and sputum IL-5 levels increased 4 and 24 hours after inhaled IL-5 (p<0.05). Serum eotaxin was raised 24 hours after intravenous IL-5 but not after inhaled IL-5 or placebo. Blood eosinophils were markedly reduced 0.5–2 hours after intravenous IL-5 (p<0.05), followed by an increase at 3, 4, 5, and 72 hours (p<0.05). Sputum eosinophils rose significantly in all three groups at 24 hours but there were no differences between the groups. Bronchial responsiveness was not affected by IL-5.

Conclusion: the effects of IL-5 appear to be mainly in the circulation, inducing peripheral mobilisation of eosinophils to the circulation without any effect on eosinophil mobilisation in the lungs or on bronchial responsiveness
0040-6376
935-940
van Rensen, E.L.J.
d9a51a2b-a24e-49ef-a3cf-6eec20189557
Stirling, R.G.
15a1c74f-6910-42ab-963a-6d9cab4760de
Scheerens, J.
0f6b8f89-f8b2-429e-a00b-b7cd85e0b102
Staples, Karl J.
e0e9d80f-0aed-435f-bd75-0c8818491fee
Sterk, P.J.
5d2d8d42-ede1-45d3-bb79-b1f1c83e9d30
Barnes, P.J.
c20c99d4-6dd6-4975-bc1a-cf3ec17f3cee
Chung, K.F.
33a71f0f-1a6b-4f86-b685-886578dfd507
van Rensen, E.L.J.
d9a51a2b-a24e-49ef-a3cf-6eec20189557
Stirling, R.G.
15a1c74f-6910-42ab-963a-6d9cab4760de
Scheerens, J.
0f6b8f89-f8b2-429e-a00b-b7cd85e0b102
Staples, Karl J.
e0e9d80f-0aed-435f-bd75-0c8818491fee
Sterk, P.J.
5d2d8d42-ede1-45d3-bb79-b1f1c83e9d30
Barnes, P.J.
c20c99d4-6dd6-4975-bc1a-cf3ec17f3cee
Chung, K.F.
33a71f0f-1a6b-4f86-b685-886578dfd507

van Rensen, E.L.J., Stirling, R.G., Scheerens, J., Staples, Karl J., Sterk, P.J., Barnes, P.J. and Chung, K.F. (2001) Evidence for systemic rather than pulmonary effects of interleukin-5 administration in asthma. Thorax, 56 (12), 935-940. (doi:10.1136/thorax.56.12.935). (PMID:11713356)

Record type: Article

Abstract

Background: interleukin 5 (IL-5) has an important role in mobilisation of eosinophils from the bone marrow and in their subsequent terminal differentiation. A study was undertaken to determine whether inhaled and intravenous IL-5 could induce pulmonary eosinophilia and bronchial hyperresponsiveness (BHR) independently of these effects.

Methods: nine mild asthmatics received inhaled (15??g) or intravenous (2??g) IL-5 or placebo in random order in a double blind, crossover study. Blood samples were taken before and at 0.5, 1, 2, 3, 4, 5, 24, and 72 hours following IL-5 or placebo, and bronchial responsiveness (PC20 methacholine) and eosinophil counts in induced sputum were determined.

Results: serum IL-5 levels were markedly increased 30 minutes after intravenous IL-5 (p=0.002), and sputum IL-5 levels increased 4 and 24 hours after inhaled IL-5 (p<0.05). Serum eotaxin was raised 24 hours after intravenous IL-5 but not after inhaled IL-5 or placebo. Blood eosinophils were markedly reduced 0.5–2 hours after intravenous IL-5 (p<0.05), followed by an increase at 3, 4, 5, and 72 hours (p<0.05). Sputum eosinophils rose significantly in all three groups at 24 hours but there were no differences between the groups. Bronchial responsiveness was not affected by IL-5.

Conclusion: the effects of IL-5 appear to be mainly in the circulation, inducing peripheral mobilisation of eosinophils to the circulation without any effect on eosinophil mobilisation in the lungs or on bronchial responsiveness

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Published date: 2001
Organisations: Faculty of Medicine

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Local EPrints ID: 374410
URI: http://eprints.soton.ac.uk/id/eprint/374410
ISSN: 0040-6376
PURE UUID: 9d41f7b3-1e8d-444d-99dc-a895947cc366
ORCID for Karl J. Staples: ORCID iD orcid.org/0000-0003-3844-6457

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Date deposited: 16 Feb 2015 14:03
Last modified: 24 Sep 2019 00:44

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Contributors

Author: E.L.J. van Rensen
Author: R.G. Stirling
Author: J. Scheerens
Author: Karl J. Staples ORCID iD
Author: P.J. Sterk
Author: P.J. Barnes
Author: K.F. Chung

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